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دکتر احمد نعمت
الهی
author
دکتر سید حسین
حسینی
author
دکتر پروانه خضرائی
نیا
author
دکتر علی
اسلامی
author
دکتر فرهنگ
ساسانی
author
عباس
گرامی
author
text
article
2004
per
Objective: Study on haematological and biochemical parameters and pathologic changes in experimental infestation to Haemonchus contortus in sheep.
Design: Experimental study.
Animals: Fourteen lambs, 5-6 months old (Two as egg donors, eight for experimental infestation and four as control).
Method: For experimental infestation, eight lambs (5-6 months old) that had no worm infestation were selected and 50000 of thirdstage larvae of Haemonchus contortus were fed to each of them. Four other lambs (control group) received placebo. In order to study the development and clinicopathologic changes, daily stool and blood samples were taken, weight was recorded, lambs were examined for clinical symptoms and slaughtered on days 12,21,35 and 60 post infestation (PT). Abomasal pH was meseared and microscopic sections were prepared.
Statistical analysis: Results were analysed by ANOVA, Duncan and “t” test.
Results: No clinical symptoms were found in the animals during this study however the body weight, monitored during 60 days P1 indicate a difference between live weight in the treatments. Statistically significant differences were observed in haemoglobulin, concentration, haematocrit, white blood cells count, neutrophil, lymphocyte, eosinophil, total protein, albumine, alpha globulins, and calcium between control and infested groups (P
Journal of Veterinary Research
The University of Tehran Press
2008-2525
59
v.
2
no.
2004
https://jvr.ut.ac.ir/article_11978_56b3d035643b8d0a9b73e5b38c66acdb.pdf
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دکتر حسینعلی
عرب
author
دکتر فردین حسین
زاده
author
text
article
2004
per
Objective: This study aimed to examine the role of nitric oxide
(NO) as mediator of non-adrenergic non-cholinergic (NANC) system in coronary arteries of sheep. Design: In vitro study.
Samples: Sixtheen hearts of sheep aged 8-10 months.
Procedure: In vitro experiments were conducted using isolated
tissue preparations. The isolated tissues were obtained from the left coronary arteries of hearts collected from lambs aged 8-10 months. They were mounted in organ bath system to record the
isometric forces by a physiograph. The tissues were suspended in
37CC Krebs’ solution bubbled with 95% oxygen and 5% CO2. Four experimental groups were prepared and the isolated tissues were treated with 5x l0 M acetyl choline (Ach), an inhibitor of NO synthethase, nitro- L- arginine methyl ester (L-NAME) and L- arginine (L-arg) as precursor for NO.
Statistical analysis: Student t-test. Results: The tissues treated with Ach contracted for a short time before they were relaxed permanently. The relaxation induced by
Ach was dependent on the viability of endothelium. The pretreatment
of tissues with L-NAME, not only decreased the relaxation induced
by Ach, but also significantly (P
Journal of Veterinary Research
The University of Tehran Press
2008-2525
59
v.
2
no.
2004
https://jvr.ut.ac.ir/article_11979_e413c497f56dc78af8cb4c151766919f.pdf
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دکتر محمود
بلورچی
author
دکتر رضا
کسروی
author
دکتر سید عبدالمحمد حسنی
طباطبایی
author
دکتر پرویز
هورشتی
author
text
article
2004
per
Objective: To evaluate the effect of a mastitis control program
(based on the mastitis prevention, elimination and monitoring) on
a herd udder health and milk quality status. Design: Field trial.
Animals: A large Holstein dairy herd of 1150 dairy cows. Procedure: 1) Evaluating the herd udder health status (by CMT and milk sampling from 19.34% of the milking cows for bacteriologic
culture, 2) Carrying out a control program (Consisted of: blitz treatment of Strep. ag., Post milking teat dipping by a 2.5% povidone Iodine solution with 5% glycerin, dry cow combination therapy,
systemic antibiotic therapy of late pregnant heifers, bedding
management, early detection, and good record keeping of clinical
cases as well as continuous monitoring of udder health and milk
quality by using bulk tank milk analysis), 3) Reevaluating the herd
udder health status (by CMT and milk sampling from 13.2% of the milking cows for culture).
Statistical analysis: Chi-square test, Analysis of variance,
Correlation coefficient, Fisher’s exact test.
Results: 1) Significant decrease in point prevalence, and incidence
rates of clinical mastitis, relative frequency of CMT-positive quarters,
relative frequency of samples from which Strep. ag., Staph. aureus
and Coliforms yielded and distribution of Strep. ag. and Staph.
aureus in clinical cases, 2) Significant increase in relative frequency of samples from which C. bovis yielded, relative frequency of samples yielding no microorganism and distribution of environmental
Streptococci, C. bovis and no growth in clinical cases, 3) Significant decrease followed by an increase in BTSCC (with an average of 225080 cells/mI), significant decrease in staph. count (with an
average of 2726 cfuJml) being noticed at the beginning of monitoring
only, and absence of significant differences in TBC, Coli. count and Strep. count (with averages of 56996, 17131 and 9948 cfulml,
respectively). In addition, in 76% of weekly bulk tank milk samples, Strep. ag. and Staph. aureus were not detected, 4) No correlation between the concentrations of major contagious pathogens and
herd BTSCC and a significant positive correlation between staph. count and BTSCC were observed.
Clinical implications: Seemingly, the achievement of furthur
improvement in mastitis status of the studied herd (i.e., eradication of Strep. ag. and effective control of Staph. aureus) would be subjected to: Continuous supervising of post milking teat disinfection
and shifting from teat spraying to dipping, monthly measurement of individual cow SCC, washing and disinfecting teat cup liners
between cows during milking and using more effective dry period
intramammary preparations as well. In addition to improve quality of herd milk, good udder preparation prior to milking is advised..
Journal of Veterinary Research
The University of Tehran Press
2008-2525
59
v.
2
no.
2004
https://jvr.ut.ac.ir/article_11980_e1b78458d1e4676ef9503a9daaad3610.pdf
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دکتر نسرین
نوذری
author
دکتر باقر
یخچالی
author
دکتر صادق
رهبری
author
دکتر غلامرضا موذنی
جولا
author
text
article
2004
per
Objective: This study was carried out for identification of Eimeria spp isolated from poultry breeder farms in Iran by PCR. Design: Pop-Gene Analysis.
Animals: Poultry breeder farms.
Procedures: A total of 114 litter samples from poultry breeder farms without previous exposure to anticoccidial vaccine were collected randomly from relatively five different climate regions
of Iran. DNA was extracted from oocysts of samples, using phenol-
chloroform and proteinase-K. Four pairs of specific primers, designated from Internal Transcribed Spacer-l(ITSI) regions of ribosomal DNA of E. acervulina, E. brunetti, E. necatrix, and E. tend/a and one pairs of universal primer BSEF-BSER which
amplify ITS 1 of different Eimeria were used in PCR assay. In tests
on purified genomic DNA from all species of Eimeria isolated from infected samples, each of four primer pairs amplified the ITS!
region of their respective target species only. The PCR products
were analyzed by agarose gel electrophoresis
Results: DNA fragments in sizes of 320 pairs (E. acervulina), 311 pairs (E. brunette), 384 pairs (E. necatrix) and 287 pairs (E. tenella)
were detected on agarose gel electrophoresis. Universal primer
pairs also amplified ITS1 of five Eimeria which isolated from
infected samples. Laboratory implications: The results of this study were showed that PCR technique is a conventional method, faster, technically
easier and very cheaper than other methods to identify the Eimeria
spp. Finally, this technique can be recommended to be a routine
work in well equipped veterinary diagnostic labs in IRAN.
Journal of Veterinary Research
The University of Tehran Press
2008-2525
59
v.
2
no.
2004
https://jvr.ut.ac.ir/article_11981_76f39f4459eebd77091598012ad9e3a4.pdf
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دکتر صمد لطف اله
زاده
author
نوید ضیایی
درونکلایی
author
دکتر تقی زهرایی
صالحی
author
دکتر سید علی
پوربخش
author
دکتر محمدرضا مخبر
دزفولی
author
دکتر غلامرضا
افشاری
author
text
article
2004
per
Objective: Study on the presence of Escherichia coli, Coccidia
and Cryptosporidium in stool samples of neonatal diarrheic calves
in Ghaemshahr and Babol, simultaneous shedding of Coccidia and Cryptosporidium with Escherichia coli in these calves, serotyping
of Escherichia coli, comparison of antibiotic sensitivity of K99 and other serotypes of Escherichia coli.
Animals: Ninety three diarrheic neonatal calves (under one month)
Procedure: Taking stool sample from rectum of the diarrheic
calves, using standard methods for detection of Coccidia and Cryptosporidium, isolation of Escherichia coli carried out by using standard bacteriological methods and serotyping and antibiotic sensitivity test of isolates. Statistical analysis: Results were reported by descriptive scales.
Results: Escherichia coli were isolated from 40.8% of diarrheic calves from which only one isolate were K99 (1.07%). In 12 samples two pathogens have been diagnosed simultaneously which in 6 samples E. coli and Coccidia (6.4%) and 6 samples E. coli and
Cryptosporidium (6.4%) have been isolated. Isolated Escherichia
co/i were resistant to many antibiotics which routinely used in
treatment of diarrhea and there was no significant difference between K99 and other isolates of Escherichia coli in antibiotic sensitivity test.
Clinical implications: From the results of this study it seems that K99+ Escherichia coli is not a common isolate in neonatal diarrheic
calves in Ghaemshahr and Babol. J. Fac. Vet Med. Univ. Tehran. 59, 2: 131-136, 2004.
Journal of Veterinary Research
The University of Tehran Press
2008-2525
59
v.
2
no.
2004
https://jvr.ut.ac.ir/article_11982_acba5ecdff6cff0b66589704e5dee850.pdf
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دکتر غلامرضا نیکبخت
بروجنی
author
دکتر حسن تاج
بخش
author
text
article
2004
per
Objective: To study the presence of spy genes among different
Salmonella serovars that isolated m veterinaiy microbiology departement
Design: Observation study.
Samples : A total of 138 Salmonella strains belonged to 9 different serovars were studied.
Procedure: In this study we applied PCR method using PG44 and
PG48 primers to amplify spvR gene in different serotypes. Results: In PCR amplification, serotyps S.abortusovis, S.dublin, S.typhimurium and S.brandburg developed the 890 bp amplicons.
S.typhi, S.senftenberg and S.bovismorb,ficans have yielded
nonspecific bands of different sizes. S.newport revealed no band
in amplification.
Conclusion: Salmonella serotypes such as typhi, senftenberg and bovismorb/lcans with nonspecific bands in PCR amplification does not share virulence plasmids. Furthermore, spvR loci could
be considered as a good marker for presence or absence of virulence
plasmids in different Salmonella serotypes.
Journal of Veterinary Research
The University of Tehran Press
2008-2525
59
v.
2
no.
2004
https://jvr.ut.ac.ir/article_11983_9ba06dd1373b87ccc34aa0d6cf98b351.pdf
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مجید
خالداری
author
دکتر پرویز
تاجیک
author
دکتر احمد افضل
زاده
author
ندا
فرزین
author
text
article
2004
per
Objective: To study the effect of CIDR on oestrous synchronization and comparison of twining percentage by using eCG and handing diet and their interaction.
Design: Completely randomized design.
Statistical analysis: Use of SPSS statistical program for parameter data and x2 and Man- Witny test for non- parameter data. Animals: A total of 69 ewes and 6 rams of Zandi breed.
Procedure: Sixty-nine Zandi ewes within 2-5 years old and weighting 47.9 (±5.6) Kg, were divided into 3 groups (group 1 fed in the pasture and two other groups were fed in their pens). In order to synchronize oestrous cycle, all ewes (in 3 groups) were treated for 13 days with a controlled internal drug-releasing device (CIDR). On the following day after removing CIDRs, ewes in groups 1 and 2 received an injection of 400 IU eCG. Two Zandi rams were then introduced to each group.
Results: The results of the present study indicated that oestrus was induced within 36-48h post CIDR withdrawal in 100, 87 and 96 percentage of ewes in groups 1,2 and 3 respectively and this differences were not significant. The percentage of parturition was 91, 83 and 87 in groups 1, 2 and 3 respectively and differences were not significant. The percentage of twining (based on ewes lambing) was significantly (P
Journal of Veterinary Research
The University of Tehran Press
2008-2525
59
v.
2
no.
2004
https://jvr.ut.ac.ir/article_11984_36200b0228af6b4f6ee9b435cfc0a677.pdf
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دکتر حسن تاج
بخش
author
دکتر حمیدرضا
ایوبی
author
دکتر ملیحه پور کبیره
عباسعلی
author
دکتر تقی زهرائی
صالحی
author
text
article
2004
per
objectives: The comparison of total and cytoplamic protein patterns
between Salmonella abortusovis strains isolated from Iran.
Design: Electrophoretic study of protiens. Samples: Salmonella abortusovis strains isolated from Iran. Procedure: Total proteins were prepared by sonication with a microbrobe. Cytoplasmic proteins were percipitated by the addition
of 3 volumes of acetone. Total and cytoplasmic extracted proteins
were analysed by SDS-PAGE and silver staining.
Results: The results showed that there were considerable differences between total protein profile of S. abortusovis strains. The cytoplasmic protein patterns were similar among the strains.
Conclusion: The protein profile differences and number of protein bands are showed in figure and tables. Infact this variations indicating a certain genotypic distance between bacterial strains that isolated
from different geographical area of Iran and may be usful for
describing the epidemiology or at least genetic relatedness of Salmonella abortusovis wild types. .1. Fac. VeL Med Univ. Tehran. 59, 2: 147-151, 2004
Journal of Veterinary Research
The University of Tehran Press
2008-2525
59
v.
2
no.
2004
https://jvr.ut.ac.ir/article_11985_ad38a2d1426acf0a262511bea0f90611.pdf
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دکتر سارنگ
سروری
author
دکتر افشین
رئوفی
author
دکتر محمد مهدی
دهقان
author
دکتر علی
اشرفی
author
text
article
2004
per
Objective: The aim of this study was to determine the frequency of diagnosed disorders or anomalies in different organs and systems and also effects of breed, age and sex on disorders incidence rate. Design: Retrospective study on cattle radiographs.
Animals: A total of 74 cattle referred to the radiology division.
Procedure: Data were collected of 171 cattle radiographs, which had been taken in the division of radiology during a 4-year period between 1998-2002. Radiographs were evaluated for site of injuries and relation of breed, sex and age with disorders incidence rate. The
information was finally gathered in charts specified for frequency
of disorders, sex, age and breed. Results: The disorders were fractures, osteomylitis, arthritis,
actinomycosis, pneumonia, colon Artesia, OCD and etc. The most
frequent disorder was fractures (16.7%) and sites of the fractures
were at lower jaw (7.1 O/), midshaft of radius and ulna, and digits
(each 4.8%).All the colon Artesia cases were detected in male calves. Frequency of disorders in immature cattle (89.4%) had a very obvious difference with matures (l0.6%).Actinomycosis was more in female.
Clinical implication: This study showed the potency of radiography
in diagnosis of cattle diseases and disorders and also percentage
of incidence probability of them in connection with factors such as sex, breed, age and site in different organs and systems of cattle.
Journal of Veterinary Research
The University of Tehran Press
2008-2525
59
v.
2
no.
2004
https://jvr.ut.ac.ir/article_11986_67f78552d6a2f4a9bbca894eda178666.pdf
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دکتر افشین
رئوفی
author
دکتر فرهید همت
زاده
author
دکتر حسن
ممتاز
author
دکتر سیمین
عقیلی
author
text
article
2004
per
Objective: To determine serological prevalence rate of infection
of sheep with IBR virus in Chaharmahal-Bakhtiary province.
Design: Cross - sectional study. Animals: Four hundred and two healthy sheep. Procedure: Serum samples were collected from 402 sheep during
four seasons of 2002-2003 in all townships of Chaharmahal -
Bakhtiary province by cluster random sampling method.
Charactristics of the animals (age, sex and history of abortion) were
recorded. Sera were tested for antibodies against IBR virus by the serum neutralization test.
Statistical analysis: Chi-square, Linear regression.
Results: Serum samples from 43 (10.7%) sheep were positive. Prevalence rate of seropositive animals in all 5 townships of the
province was estimated. No relationship was found between township, sex, season, previous record of abortion and IBR infection
in sheep. Linear regression test indicated a relationship between
age and prevalence rate of infection (r = 0.94).
Journal of Veterinary Research
The University of Tehran Press
2008-2525
59
v.
2
no.
2004
https://jvr.ut.ac.ir/article_11987_25362c2518dce7c8099eaec4ce8671b9.pdf
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دکتر داود
شریفی
author
دکتر جلال
بختیاری
author
دکتر سید حسین
مرجانمهر
author
دکتر مریم زرین
مهر
author
text
article
2004
per
Objective: To evaluate acupuncture therapeutic regimens on histomorphological changes of muscle injury healing in dog. Design: Prospective randomized experimental study. Animals: A total of 10 young adult clinically normal male cross
breed dogs weighing 26±4.4 kg and 30±4.2 months old.
Methods: All dogs were anaesthetized with 5% thiopental sodium and maintained with 1% halothane. A piece of 3 cm in length, 1 cm in width and 1 cm in depth of right biceps femoris was removed in all of them. Then they were divided into two groups of 5 animals
each. Acupuncture treatment was given daily for 10 minutes for
two weeks in experimental group by locating acupoints (GB3O,
BL4O, ST36), where as no treatment was given in control group till end of 60 days of observations period. Biopsy sample was collected from each case for histomorphological study which
were stained with H & E stain.
Result: There was the least inflammatory cells, with regular and parallel arrangement of collagenous fiber in experimental group as compared to control one in which sample of inflammatory cells, with irregular unparallel arrangement of collagenous fiber was
quite obvious.
Conclusion: Acupuncture therapeutic regimens for two weeks period had positive and stimulatory effect on muscle tissues formation
comparatively with the least inflammatory cells with regular and
parallel collagen fiber arrangement in experimental group.
Clinical relevant: Acupuncture as a physical method of therapy is quite effective on muscle healing and faster remodelling of muscle
Journal of Veterinary Research
The University of Tehran Press
2008-2525
59
v.
2
no.
2004
https://jvr.ut.ac.ir/article_11988_a5d5be2c86e9824e142d2f5fbcb16b9a.pdf
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دکتر شهرام
جمشیدی
author
دکتر سعید
بکائی
author
دکتر کمال الدین
آراسته
author
text
article
2004
per
Objective: Comparison of intraocular pressure alternations measured
by Schiotz tonometry before and after induction of anesthezia by
ketamine and acepromazine in stray cats. The effects of factors like age, sex and iris color on intraocular pressure were also studied. Design: Uncontrolled clinical trial.
Animals: Hundred stray cats were used in the study.
Material and methods: Hundred stray cats were collected from different portions of Tehran by a special cage designed to do this. To improve the accuracy of data in each case measurement was
performed three times and it’s average was recorded as lOP in that
animal. Furthermore, because in many clinical examinations
especially when the animal suffer from pain due to glaucoma use of anesthesia is obligatory and to assess the effect of anesthesia on
lOP, all of the cats were anesthetized by ketamine hydrochloride
and acepromazine in traditional doses and lOPs were measured again in this situation.
Statistical analysis: In this study statistical analysis were performed by independent t test, paired t test, one way ANOVA and tookey as complementary test.
Results: In this study, the mean of lOP was 17.5 ± 0.46 mmHg. Furthermore, the results of this study showed that sex and color of
iris had no significant effect in lOP, but in respect to age based on
statistical ANOVA test, the difference was significant. Younger animals had higher values of lOP compared with older cats. Beside this, the results of this study showed that anesthesia with ketamine
and acepromazine in cats can lead to increase in IOP and X =
formula, as an index, can be used for lOP determination in situations that anesthesia performance is obligatory (x = corrected lOP value before anesthesia induction, y = acquired lOP value after
anesthesia).
Discussion: From the factors evaluated in this study, just age had a significant influence on lOP. Beside this for determination of glaucoma especially when use of ketamine and acepromazine are
obligatory and there is considerable pain in the patient and physical restraint is impossible, this could lead in increase of lOP in affected
animals. To evaluate lOP properly in this conditions use of correction
coefficients could be helpful.
Journal of Veterinary Research
The University of Tehran Press
2008-2525
59
v.
2
no.
2004
https://jvr.ut.ac.ir/article_11989_aa05e4f7c8189c1faa4426523776db75.pdf
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دکتر شاهرخ رنجبر
بهادری
author
دکتر علی
اسلامی
author
دکتر بهنام
مشگی
author
دکتر سعید
پورحسینی
author
text
article
2004
per
Objectives: A study on the prevalence of stray cats infected with
parasitic helminthes in Tehran.
Design: Cross sectional study. Animals: One hundred stray cats captured from different urban
areas of Tehran.
Methods: One hundred stray cats were trapped from different
geographic regions of Tehran and were necropsied. Different organs
including: kidney, heart, liver, lungs, gastrointestinal tract and abdominal cavity were inspected for helminthic infection.
Statistical analysis: X2 test was used to show the relationship between different factors and parasitic infection and E ta coeffeciency
was used to presenting effects of these factors on the infection.
Results: Four species of helminthes including 3 nematodes, Toxocara cati (23%), Physaloptrapraeputialis (7%) and Toxascaris leonine
(2%) and one cestode, Diplopylidium nolleri (5%) were found in
gastrointestinal tract and only this organ was found infected in
examined cats.
Conclusion: Although for some species (e.g. T.cati 23%), the percentage of infection was relatively high, but with regard to the
mild intensity of the collected worms, they could not cause clinical
or subclinical symptoms. On the other hand, considering the contact
between cat and man especially children, through courtyard
environment or direct contact and visceral or ocular larva migration
produced by ascarids of dog and cat, the report of two species of Ascaris in the present study could be a health hazard for human
especially children.
Journal of Veterinary Research
The University of Tehran Press
2008-2525
59
v.
2
no.
2004
https://jvr.ut.ac.ir/article_11990_d806278660270c1483a05f32c93fb36a.pdf
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دکتر فرهید همت
زاده
author
دکتر هادی
کیوانفر
author
دکتر مریم
بادوام
author
محمد
طاهری
author
text
article
2004
per
Objective: This study was carried out to find the progressive
cellular changes developed in cell culture with cytopathic (cp) and
noncytopathic (ncp) pestiviruses isolated from cow and sheep. Design: Observational study. Procedure: Following inoculating the R-BK cell cultures in Lighton tube with NADL strain of BVD, a cp strain of Border disease virus
and a nocytopathic BVD, the cells were stained with FA and
observed with IF microscope. In inoculated cultures with cp and
ncp pestivirus obvious changes were present at the first day and multiple focal expression of viral antigens were seen. Results: In the second day in cp inoculated cultures, the
morphological changes began and antigenic spread were more
intensive. In the 3rd day the CPE foci were seen in different parts
of the cultured cells with high concentration of antigen around the CPE sites. The cells started to detach and became rounds and finally in the 4th and 5th day, almost all cells were antigen positive and
developed CPE. However, those cultures inoculated with noncytopathic
strain of BVD, in daily observation the antigenic spread was too
slow and up to 6 days post inoculation no CPE were observed.
Journal of Veterinary Research
The University of Tehran Press
2008-2525
59
v.
2
no.
2004
https://jvr.ut.ac.ir/article_11991_bb8572dbfe902e378445432356457603.pdf
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دکتر ابوالفضل
کامکار
author
دکتر نوردهر
رکنی
author
دکتر عبدالمجید
چراغعلی
author
دکتر هدایت
حسینی
author
دکتر مهران رضائی
مجاز
author
دکتر سعید
بکایی
author
دکتر ایرج
نوروزیان
author
دکتر عبدالحکیم عبداله
زاده
author
text
article
2004
per
Objective: Determination of Nitrite residues in different types and brands of meat products in order to assessment for setting up the standards and measures for this preservative in Iran.
Design: Descriptive, analytical study based on random sampling. Number of samples: A total of 250 meat product samples from (Sausages).
Procedure: Meat product samples (Sausages) produced by factories in Iran, selected randomly and the nitrite residues were analyzed in all samples by spectrophotometric method.
Statistical analysis: Means and standard errors nitrite residues were determined and then analyzed by one-way analysis of variance method.
Results: The results of the experiments on the different types and brands of meat products showed that level of nitrite residues were between 1-108 p.p.m and according to one-way analysis of variance differences between the means of nitrite residues in red meat Sausages were higher than the other meat products (P
Journal of Veterinary Research
The University of Tehran Press
2008-2525
59
v.
2
no.
2004
https://jvr.ut.ac.ir/article_11992_cbf8b408da9c40918ba71739fe1d9abe.pdf
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دکتر حمیدرضا
حدادزاده
author
دکتر محمد راضی
جلالی
author
دکتر پروانه خضرائی
نیا
author
محمد
طاهری
author
دکتر عبدالرحمن
راسخ
author
text
article
2004
per
Objective: Evaluation of IFAT and abattoir methods for identifying and study of buffalo sarcocystosis(Sarcocvstis fusiformis). Samples: A total of 398 serum samples were taken from buffaloes before slaughtering for IFAT studing the rate of sarcocystis infections and the results compared with meat inspection and laboratory finding (macro and micro cyst).
Procedure: Before slaughtering, blood samples were taken from jagular vein for serological examination by IFA method. After slaughtering, esophagus, diaphragm, heart and skeletal muscles were examined for macroscopic cyst of sarcocystis .For microscopic cysts, the samples were taken from each one of these tissues for impression smear (Dob smear). The macro cysts were identified as S.fusjformis. Bradizoites of this sarcocyst were used as antigen in IFAT and rabbit antibuffalo conjugated serum for this test was prepared in central laboratory of faculty of veterinary medicine, University of Tehran (Dr.Reza Rastegar central laboratory) using standard method.
Results: The results showed that macroscopic and microscopic infection rates of animals is 18.6% and 53,5°/a respectively. In this study, maximum rate of infection include macroscopic and microscopic finding was in eosophagus and minimum in heart muscle. Any significant differences were observed in infection rates due to sex: The infection rate in adult group was significantly more than young buffaloes. A significant correlation was observed between antibody titer and the rate of macroscopic and microscopic infection (P
Journal of Veterinary Research
The University of Tehran Press
2008-2525
59
v.
2
no.
2004
https://jvr.ut.ac.ir/article_11993_14fb1fc91bba3c528dfaeb2372145c60.pdf
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دکتر تقی زهرایی
صالحی
author
دکتر ذوالفقار
رجبی
author
دکتر مهرداد مدیر
صانعی
author
دکتر سعید
بکائی
author
text
article
2004
per
Objective: Evaluation of the efficacy of inactivated monovalent and polyvalent vaccines prepared from F. coli serotypes in poultry in Iran.
Design: Randomized completely Design.
Animals: Three hundred sixty Arian broiler chicks.
Procedure: In this study monovalent vaccines 078:K80 and polyvalent vaccine were prepared, using native and virulent E.coli serotypes of078:K80, 0128:K67, 02:Kl, 0l24:K82, 01 19:B14 and appropriate adjuvant ALK (S04)2.12H20 and KOH. Three hundred sixty day old broiler chicks were randomly divided into four treatment and one control groups. Birds in each group were injected with 0.5 ml (1.5 x 0) of one of four prepared vaccines by subcutaneous administration in second week and intramuscular administration in third and fourth weeks of age. Before challenge serum antibody titers were measured by the tube and slide agglutination test. Ten days after the last vaccination chicks were challenged with virulent strain of 078:K80, 02K 1 and 012X:K67 E. coli serotypes.
Statistical analysis: Analysis of variance and Scheffe’s test. Results: More than 95 percent of chickens in control group showed colibacillosis and 70% of them were died after challenge with 078:K80, while in vaccinated groups just 3.7 oo mortality was observed. Live and dead challenged chicks of control group had typical lesions of colibacillosis. No adverse effects were noted on growth rate that were vaccinated with monovalent vaccines. Conclusion: The results of this study revealed that inactivated monovalent and polyvalent vaccines prepared from endemic E. coli serotypes are immunogenic and protective in broiler chicks against virulent E. coli. No cross protection was shown among heterologous serotypes. The vaccines do not have any effects on growth rate or carcasse quality in vaccinated chicks. Thus we suggest using the endemic E. coli vaccine to protect broiler chicks against colibacillosis.
Journal of Veterinary Research
The University of Tehran Press
2008-2525
59
v.
2
no.
2004
https://jvr.ut.ac.ir/article_11994_1f491a5a95000e19582381cb6734fd40.pdf
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دکتر امید
دزفولیان
author
دکتر داریوش
شیرانی
author
دکتر مسعود سلک
غفاری
author
دکتر رضا
نقشینه
author
دکتر مهدی
مرجانی
author
text
article
2004
per
Objectives: Clinical case report of leydig cell tumor in dog.’
Design: Clinical case study.
History: An eleven year old male German shepherd dog was reffered, small animal hospital of faculty of veterinary medicine
because of Anorexia. Because of poor body condition, the animal was euthanised and submitted for necropsy examination. Based on
the light & electronic microscopic observation, leydig cell tumor
was confirmed.
Clinical implications: Occurrence of tumor in dog is without
clinical findings, therefore maybe testicles are not necroptize at
necropsy. Thus the rate of tumor, must probably be higher than it is reported.
Results: Histopathology and uttrastructural observations, confirmed
the leydig cell tumor.
Journal of Veterinary Research
The University of Tehran Press
2008-2525
59
v.
2
no.
2004
https://jvr.ut.ac.ir/article_11995_1f04b3266aaae94bfdedc76c965bb2e1.pdf
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دکتر سید رضا
قاضی
author
دکتر سید هادی
منصوری
author
دکتر ملیحه الزمان
منصفی
author
text
article
2004
per
Objective: Study of anatomical position of termination of the spinal cord in male cat. Animals: Fifteen male cat in 2 prenatal groups of mid stage fetus
(37d) and late stage fetus and 3 postnatal groups of newborn, sexually
adult (6 m) and adult age (>ly/o) and 3 animals in each group.
Procedure: Fixation of spinal cord with injection of and suspension inlO% formalin, laminectomy of vertebral column and definition of spinal cone on related to vertebral column by pin. Results: The results indicated that, as in the mid and late stage
fetuses, the spinal cord terminated at anterior one third of Co1
and posterior one third of 53, respectively but in newborn, sexually adult stage and adult age at posterior, middle and anterior one third of 2’ respectively.
Discution: Thus with increase of age the terminal portion of spinal
cord were more anterior and this showed that the vertebral column
had been grown faster than that the spinal cord.
Journal of Veterinary Research
The University of Tehran Press
2008-2525
59
v.
2
no.
2004
https://jvr.ut.ac.ir/article_11996_344dd1076d931840ce6461bb0f9d76c0.pdf