TY - JOUR ID - 18883 TI - تشخیص بروسلوز با استفاده از سیستم کشت خون ‌ BACTECو تائید نتایج آن با PCR JO - مجله تحقیقات دامپزشکی (Journal of Veterinary Research) JA - JVR LA - fa SN - 2008-2525 AU - نژاد, پرویز مالک AU - دوگانه, هادی پیری AU - امیرزرگر, علی اکبر AU - جعفری, سیروس AU - زاده, بهرام فتح اله AD - Y1 - 2007 PY - 2007 VL - 62 IS - 4 SP - EP - KW - بروسلا KW - بروسلوز KW - سیستم BACTEC KW - کشت خون. DO - N2 - Abstract: Brucellosis is one of the most common zoonotic diseases in Iran. Growth of Brucella is slow and blood cultures of these bacteria are time-consuming via classical methods. We try to evaluate BACTEC 9120 system capacity in order to detect of bacteremia due to Brucella spp and to confirm isolated bacteria by PCR. Blood culture sample of 102 suspected patients evaluated by BACTEC 9120 system. They were subcultured when the machine detected their growth; if not; blind subcultures were performed on days 7, 14, 21 and 28. Forty-one of 102 suspected patients showed bactermia. Isolation rate of Brucella was 40.2%. All patients were detected by BACTEC 9120 system. All positive blood culture was detected via BACTEC 9120 and blind subcultures. No positive blood culture bottles were missed by the system. Our data obtained by using the BACTEC 9120 system indicates a more rapid detection of Brucella than conventional methods. Key words: brucellosis, BACTEC system, blood culture. UR - https://jvr.ut.ac.ir/article_18883.html L1 - https://jvr.ut.ac.ir/article_18883_78c70b6858a15b04e9ed240ee8067a0c.pdf ER -