TY - JOUR ID - 23938 TI - ایجاد تجربی بیماری آنتریت در جوجه های گوشتی با استفاده از جدایه های PERFRINGENS CLOSTRIDIUM از موارد حاد بیماری در ایران JO - مجله تحقیقات دامپزشکی (Journal of Veterinary Research) JA - JVR LA - fa SN - 2008-2525 AU - شجاعدوست, بهرام AU - پیغمبری, سید مصطفی AD - گروه علوم درمانگاهی دانشکده دامپزشکی دانشگاه تهران Y1 - 2008 PY - 2008 VL - 63 IS - 2 SP - 128 EP - 132 KW - آنتریت نکروتیک KW - بیماری تجربی KW - جوجه گوشتی KW - کلستریدیوم پرفرینجنس DO - N2 - To establish an stable challenge system for induction of necrotic enteritis (NE) in broiler chickens, different potential factors and predisposing conditions were imposed in 6 separate different experiments using 525 day-old chicks in total. Despite the presence of some predisposing factors and using 4 isolates of Clostridium perfringens ( CP ) from acute and severe NE outbreaks as challenge bacteria, the disease was not successfully reproduced in the first 4 experiments. In the 5th and 6th experiments, the predisposing conditions were changed and each bird was challenged with 3 ml (3×108 CFU/ml) of CP live culture via oral route and also the feed mixed with CP suspension 2 times per day and for 5 consecutive days. Clinical signs and mortalities, and lesions associated with NE were observed in the later two experiments. This study showed that by stressful nutritional and management procedures such as increased stocking density and high levels of wheat and fish meal; induction of relative immunosuppression; using sufficient CP concentration and appropriate ways of its multiplication; and applying challenge bacteria isolated from acute outbreaks, NE may be experimentally induced in broiler chickens. UR - https://jvr.ut.ac.ir/article_23938.html L1 - https://jvr.ut.ac.ir/article_23938_e69a23e09cce75e5738d626e6e6efbb6.pdf ER -