2024-03-29T12:24:31Z
https://jvr.ut.ac.ir/?_action=export&rf=summon&issue=2409
مجله تحقیقات دامپزشکی
(Journal of Veterinary Research)
J Vet Res
2008-2525
2008-2525
1387
63
2
مطالعه پراکنش بیماری نکروز عفونی بافت های خونساز(IHN) در پنج استان عمده تولید کننده بچه ماهی قزل آلای رنگین کمان کشور با استفاده از تکنیک های آنتی بادی درخشان به روش غیر مستقیم (IFAT) و واکنش زنجیره ای پلی مراز (nested-RT-PCR)
مهدی
سلطانی
مهدی
سلطانی
فرهید
همت زاده
بهرام
کاظمی
حسینعلی
ابراهیم زاده موسوی
شناسایی مراکزتکثیر آلوده به ویروس بیماری نکروز عفونی بافت های خونساز(IHN) در پنج استان عمده تولید کننده تخم چشم زده و بچه ماهی قزل آلای رنگین کمان کشور شامل مازندران، چهارمحال و بختیاری ، کهگیلویه و بویر احمد ، لرستان و فارس با استفاده از تکنیک های IFATو nested-RT-PCR و مقایسه این دو تکنیک با یکدیگر مورد مطالعه قرار گرفته است. بافت های کلیه، کبد و طحال بچه ماهی قزل آلای رنگین کمان طی شرایط استریل در محل مزارع جداسازی و نهایتاً در فریزر 70- درجه سانتیگراد نگهداری شد. به منظور افزایش اختصاصیت واکنش RT-PCR ، یک واکنش ثانویه nested-PCR روی محصول اولیه انجام گرفت. پس از هشت ماه آزمایش های فوق الذکر مجدداً روی نمونه های مثبت این مزارع تکرار شد تا حساسیت این دو روش در طی زمان برای نمونه های قدیمی مشخص گردد. نتایج حاصله نشان داد که تمامی پنج استان یاد شده آلوده به ویروس IHN هستند ( چهار مزرعه از بیست و هفت مزرعه نمونه برداری شده). نتایج بدست امده نشان داد که هر چند IFAT حساسیت قابل قبولی برای شناسایی IHNV در مراحل اول آلودگی از خود نشان می دهد با این وجود آزمایش nested-RT-PCR واجد توانایی بالاتر از RT-PCR و حساسیت بالاتری نسبت به HFAT بخصوص برای نمونه های نگهداری شده در فریزر به مدت چندین ماه می باشد.
بیماری نکروز عفونی بافت های خونساز
قزل آلای رنگین کمان
2008
06
21
99
105
https://jvr.ut.ac.ir/article_23933_400ec1803e0c5f2a587e4406a4becb28.pdf
مجله تحقیقات دامپزشکی
(Journal of Veterinary Research)
J Vet Res
2008-2525
2008-2525
1387
63
2
بررسی کارآیی درمان مقدماتی با آنتاگونیست GnRH در سوپراوولاسیون بز
فرید
حیدری دزفولی
فرامرز
قراگوزلو
مهدی
وجگانی
سید مرتضی
میرترابی
مرتضی
دلیری
تاثیر استفاده کوتاه مدت از آنتاگونیست GnRH به عنوان تیمار مقدماتی در برنامه سوپراوولاسیون بز مورد ارزیابی قرار گرفت. آنالیز یافته ها با ازمون t-test نشان داد که تیمار مقدماتی با آنتاگونیست GnRH باعث بهبود تعداد جنین های استحصالی می شود( 05/0>P) ولی اختلاف معنی داری از نظر تعداد اجسام زرد و فولیکول های کیستی بین دو گروه درمان و کنترل مشاهده نشد ( 05/0
آنتاگونیست GnRH
اجسام زرد
بز
جنین استحصالی
سوپراوولاسیون
2008
06
21
107
111
https://jvr.ut.ac.ir/article_23934_5b1384db2be7d02d7235a5b4aa06b4a3.pdf
مجله تحقیقات دامپزشکی
(Journal of Veterinary Research)
J Vet Res
2008-2525
2008-2525
1387
63
2
تاثیر پرتودهی با اشعه گاما و نگهداری در انجماد بر روی خواص حسی، شیمیایی و باکتریایی گوشت ماهی
علیرضا
صفاریان
نوردهر
رکنی
افشین
آخوند زاده بستی
علیرضا
باهنر
حسینعلی
ابراهیم زاده موسوی
نگین
نوری
In this study the effects of gamma irradiation and frozen storage on sensory, chemical and microbial
quality of fish fillets was investigated. Hypophtalmyctis molitrix were equally divided to 12 fillests,
then these 12 fillets were equally arranged in 4 groups (1-4). One group considered as control with no
treatment and the other 3 groups was treated by 0.75, 3 and 5 KGy of gamma irradiation, respectively.
All of the groups were kept in -18?c for 3 month. All of the samples were examined for microbial,
chemical and sensory examinations at day zero and 3 months later. The data were analysed by ANOVA
(Kruskal-Wallis) using SPSS 10. The results showed treatment with 0.75 KGy of gamma irradiation
plus frozen storage in extension of shelflife of fish fillets without any unacceptable effects on sensory
and chemical characteristics of them.
اشعه گاما
انجماد گوشت ماهی
پرتو دهی
مدت زمان نگهداری
2008
06
21
113
115
https://jvr.ut.ac.ir/article_23935_4b48eb243bdf0e82f474c7cbcd13bc8b.pdf
مجله تحقیقات دامپزشکی
(Journal of Veterinary Research)
J Vet Res
2008-2525
2008-2525
1387
63
2
مطالعه مراحل رشد درون بافتی انگل های میکسوبولوس کارونی و میکسوبولوس پرسیکوس و معرفی سه میزبان جدید
محمود
معصومیان
مهدی
چوبچیان
جمیله
پازوکی
عیسی
شریف پور
بهیار
جلالی
Survey on developmental stages of Myxobolus karuni and M. persicus in the gills of Barboid fishes
from Mezopotamian part of Iran was done. A total 296 fish specimences were examined. Random
sampling was carried out during 2002- 2004 from Karoun, Karkheh Rivers and Shadgan Lagoon. The
fishes transported alive to the laboratory, their gills were fixed and stained. Myxobolus karuni
developed histozoic, the plasmodia were found inside the blood vessel, it developed in the epithelial
cells of primery filaments, the spores are large and ellipsoidal in shape. M.persicus starts and finished
the plasmodia in epithelial and endothelial of the secondary filaments, the spores are oval in shape. Up
to now, the location of infection of few species of gills Myxobolus Spp. are known. According the
results of this study Barbus pectoralis, B. barbulus and B. esocinus are new hosts for Myxobolus karuni
and M persicus.
آبشش
ایران
میزبان های جدید
میکسوبولوس پرسیکوس
میکسوبولوس کارونی
2008
06
21
117
122
https://jvr.ut.ac.ir/article_23936_59784aa19a528af06b13b0038aca02bd.pdf
مجله تحقیقات دامپزشکی
(Journal of Veterinary Research)
J Vet Res
2008-2525
2008-2525
1387
63
2
تعیین گونه های کنه در ماسنتور در برخی از مناطق ایران
صدیقه
نبیان
صادق
رهبری
پرویز
شایان
حمید رضا
حداد زاده
This study conducted during 3 years period (2002-2004). Tick sampling was randomly carried out
from domestic animals during seasonal activity of ticks from six provinces of Iran. 2170 ticks from
151 cattle , 629 sheep, 336 goats and 32 camels were collected proin 30 prosseces with a mean number
of 1.9 tick per animal. 209 Dermacentor ticks collected from six provinces( Kordestan, Ardebil, East
Azarbaiejan, Zanjan, Khorasan and Semnan) that included 23% of collected tick population in those
provinces. The diversity of Dermacentor is restricted to three species; D.niveus (50%), D.marginatus
(27%) and D.raskemensis (23%). The maximum occurance of D.raskemensis , D.niveus, D.
marginatus, were occurred in provinces of Semnan, Khorasan and Kordestan, respectively. It can be
concluded that livestock had almost different pattern of tick species in any localities thus distribution
of D.raskemensis , D.niveus, D. marginatus, has been confirmed this matter.
انتشار جغرافیایی و ایران
درماسنتورراسکمنسیس
درماسنتور مارژیناتوس
درماسنتورنی وئوس
2008
06
21
123
126
https://jvr.ut.ac.ir/article_23937_571cf2f01532717432da4cbd1986b688.pdf
مجله تحقیقات دامپزشکی
(Journal of Veterinary Research)
J Vet Res
2008-2525
2008-2525
1387
63
2
ایجاد تجربی بیماری آنتریت در جوجه های گوشتی با استفاده از جدایه های PERFRINGENS CLOSTRIDIUM از موارد حاد بیماری در ایران
بهرام
شجاعدوست
سید مصطفی
پیغمبری
To establish an stable challenge system for induction of necrotic enteritis (NE) in broiler chickens,
different potential factors and predisposing conditions were imposed in 6 separate different
experiments using 525 day-old chicks in total. Despite the presence of some predisposing factors and using 4 isolates of Clostridium perfringens ( CP ) from acute and severe NE outbreaks as challenge bacteria, the disease was not successfully reproduced in the first 4 experiments. In the 5th and 6th experiments, the predisposing conditions were changed and each bird was challenged with 3 ml (3×108 CFU/ml) of CP live culture via oral route and also the feed mixed with CP suspension 2 times per day and for 5 consecutive days. Clinical signs and mortalities, and lesions associated with NE were observed in the later two experiments. This study showed that by stressful nutritional and management procedures such as increased stocking density and high levels of wheat and fish meal; induction of relative immunosuppression; using sufficient CP concentration and appropriate ways of its multiplication; and applying challenge bacteria isolated from acute outbreaks, NE may be experimentally induced in broiler chickens.
آنتریت نکروتیک
بیماری تجربی
جوجه گوشتی
کلستریدیوم پرفرینجنس
2008
06
21
128
132
https://jvr.ut.ac.ir/article_23938_e69a23e09cce75e5738d626e6e6efbb6.pdf
مجله تحقیقات دامپزشکی
(Journal of Veterinary Research)
J Vet Res
2008-2525
2008-2525
1387
63
2
تاثیر آنتی بادی استخراج شده از تخم مرغ و پروبیوتیک در پیشگیری ازعفونت سالمونلا انتریتیدس در جوجه های گوشتی
شعبان
رحیمی
شعبان
رحیمی
تقی
زهرایی صالحی
To evaluate the effect of probiotic (Primalac) and Salmonella enteritidis-specific IgY on
prevention of Salmonella enteritidis infection in broiler chickens, four 33 week-old Single Comb
White Leghorn hens were hyperimmunized with Salmonella enteritidis (SE) whole cell antigens
obtained by ultrasonication and administrated at a protein concentration of 500 ?g/ml after
centrifugation. Primary immunization was performed with 250?g of the antigen prepared in equal
volume of Freunds complete adjuvant and saline. Booster injections were done each 14 days for twice,
using incomplete Freunds adjuvant. Bleedings were performed 20 days after first injection and eggs
were collected. The presence of anti-Salmonella antibody IgY and IgG in egg yolk and serum
respectively, was monitored by ELISA, during the immunization period. Body weight, feed intake and
feed conversation were determined. Then two hundred forty male “Ross “day-old chicks were
randomly assigned to 8 groups and 3 replications of 10 birds were grown for 56 days of experiment.
Eight experimental groups identified with, S, P, A, SP, SA, AP, SPA, C. Four birds from four challenged
groups(S), were orally inoculated with 0.5 Ml of S. enteritids that contained 1×106 cfu / ml on day 7.
The groups that supplemented with antibody (A), received 15 ml of yolk contained antibody(1.5
ml/bird/day), from day 1 to end of the experiment. The probiotic treated groups(P) were received
probiotic, 0.1% of feed and 0.5% of feed, during 1-21 and 22-56 days of experimental period
respectively. One group as control© did not receive any treatment of probiotic and antibody.
الایزا
ایمونوگلوبین G
ایمونوگلوبین Y
پروبیوتیک
سالمونلا انتریتیدیس
2008
06
21
133
139
https://jvr.ut.ac.ir/article_23939_70780d0eed698c5169138850f780199e.pdf
مجله تحقیقات دامپزشکی
(Journal of Veterinary Research)
J Vet Res
2008-2525
2008-2525
1387
63
2
تشخیص آنتی ژن های توموری در عقده لمفاوی گاو مبتلا به لنفوسارکوم ویروسی با روش رسوب ایمنی
غلامرضا
نیکبخت بروجنی
سید مهدی
امام
ندا
برجسته
In this study One lymph node from cow with viral lymphosarcoma and one lymph node from
healthy cow were analyzed by three different methods for protein extraction, include simple
homogenizing in PBS, sonication and phenol extraction. Tumor antigen detection relies on
immunoprecipitation followed by SDS-PAGE. In this experiment Streptococcus pyogenes was used
for purification of immune complexes. Eletrophoretic patterns were obtained using reduced and non
reduced buffers. In PBS homogenization 3 distinct antigens (39, 32 and 30 kDa) were observed. When
sonication or phenol extraction were used, 5 (72, 48, 42, 32 and 30 kDa) and 6 (104, 77, 54, 32, 30 and
26, 5 kDa) distinct antigens were observed, respectively. This study showed that the ability of antigen
detection mainly depends on protein extraction procedure and antigen-antibody equilibrium achieved
by quantification of precipitins. Immunoprecipitation could be used successfully for study of tumor
antigens in bovine leukosis.
آنتی ژن های توموری
رسوب ایمنی
لکوزگاوی
لمفوسارکوم
2008
06
21
141
146
https://jvr.ut.ac.ir/article_23940_7164b59215bbed7c527dafc1f1e4015d.pdf
مجله تحقیقات دامپزشکی
(Journal of Veterinary Research)
J Vet Res
2008-2525
2008-2525
1387
63
2
ارزیابی مقایسه ای برداشت رحم و تخمدان به روش جراحی لاپاروسکوپیک و باز در سگ
جلال
بختیاری
سارا
مکرم
علیرضا
خلج
داوود
شریفی
جواد
اشرفی هلان
آذین
توکلی
Laparoscopic and open ovariohysterectomy were compared for the following parameters: surgical
time, incision length, suture number, saturation of oxyhemoglobin (SpO2), heart rate, complications,
CBC findings, Melbourne pain scale (MPS) and gross pathology. MPS pain scores were evaluated
using Mann-whitney U Test. Other parameters were evaluated using Studen's t test. There were no
significant differences in SPo2, heart rate, surgical complications, MPS pain scores and CBC, where
as the blood loss was significantly (P
ارزیابی درد
برداشت رحم و تخمدان
سگ
لاپاروسکوپی
2008
06
21
147
151
https://jvr.ut.ac.ir/article_23941_a4fa409460351e5ab7bc0b872d69fe54.pdf
مجله تحقیقات دامپزشکی
(Journal of Veterinary Research)
J Vet Res
2008-2525
2008-2525
1387
63
2
بررسی رابطه ضایعات هیستوپاتولوژیک با تغییرات آنزیم های بافتی در ماکیان مبتلا به بیماری مارک
سعید
نظیفی
عزیزاله
خداکرم تفتی
مریم
انصاری لاری
مریم
کارگر
The aim of the present study was to determine the relationship between histopathological lesions
and tissue enzymes in chickens affected by Marek's disease.Five apparently healthy chickens(as
control group)and 25 chickens affected by Marek's disease were selected. After consideration of
history and gross lesions, tissue samples were collected from kidney, liver, heart, ovary, pectoral
muscle, spleen, crop, proventriculus and bursa of fabricius for histopathological and tissue enzyme
studies. In tissue samples, the activity of aspartate aminotransferase (AST), alkaline phosphatase
(ALP), lactate dehydrogenase (LDH), creatine kinase (CK), superoxide dismutase (SOD)and
glutathione peroxidase (GPX)was measured. Histopathologically, lymphomatous lesions in visceral
organs were included in local or diffuse ple omorphic small to medium lymphocytes, lymphoblasts,
marek cells and rarely plasma cells. The results indicated that the activities of AST, LDH, CK, SOD
and GPX were decreased in the crop of affected chickens. The activities of CK, LDH and AST in the
liver tumors and the activities of LDH, AST, ALP, SOD, CK and GPX in pectoral muscles were
increased. In proventriculus, the activities of AST, ALP, LDH and SOD were shown to be increased,
but the activity of CK was decreased. The activities of AST and SOD in the ovary lesions and the
activities of SOD, AST, CK and LDH in the heart lesions showed an increase. Fluctuations in the
activity of enzymes in different tissues showed that measurement of these enzymes can not be used as
a tumor marker in the diagnosis of Marek's disease.
آنزیم
بافت
بیماری مارک
جوجه
هیستوپاتولوژی
2008
06
21
153
158
https://jvr.ut.ac.ir/article_23942_d776dc3bfd5cd2e4aa7b65e70fe3bc20.pdf
مجله تحقیقات دامپزشکی
(Journal of Veterinary Research)
J Vet Res
2008-2525
2008-2525
1387
63
2
اثر تزریق داخل صفاقی هیستیدین بر میزان دفع مدفوع در خرگوش
غلامرضا
وفایی سیاح
اسماعیل
تمدنفرد
Histidine at the doses 30 and 60ml /kg had no effect when injected at 7 :OOh, or at 19:00h. Histidine
at the doses of 120, 240 and 480 mg / kg decreased defecation at the first 8 and 24 hours post injection,
respectively, when injected at 07:00h. The amino acid at the doses of 120, 240 and 480 mg / kg
decreased the number of fecal pellets at 2, 8 and 24 h post injection, respectively, when injected at
19:00h. Suppressive effect of histidine in the light period was more intense and lasted longer than that
in the dark period. The mean number of pellets for each individual prior to the interventional study was
recorded as interval negative control.
پلت های مدفوع
خرگوش
هیستیدین
2008
06
21
159
162
https://jvr.ut.ac.ir/article_23943_38a66a6fa01cd3635f3c2eb80ef2b275.pdf
مجله تحقیقات دامپزشکی
(Journal of Veterinary Research)
J Vet Res
2008-2525
2008-2525
1387
63
2
بررسی آلودگی های کرمی دستگاه گوارش سگ های خانگی شهر شیراز
سعید
بکایی
محمد
موذنی
مرتضی
رودگر
Gastrointestinal helminthes in domestic dogs in Shiraz and the effect of age, sex, anthihelminth
drugs, use of dog (pet or guard) and food on the infection rate were studied in 105 fecal samples.
Samples were collected directly from the rectum and examined by floatation method. Data was
analyzed by Chi square test and Fisher's exact test. No significant differences were found in male
and female dogs and also different age groups. Higher infection rate was observed in guard dogs in
comparison with pet dogs. The infection rate was higher in dogs feeding on viscera or uncooked
food. The awareness of owner's about the zoonotic helminthes infection was unsatisfactory.
However according to risk of communicable helminthes educational programs for owners are
strictly recommended.
انگل کرمی
دستگاه گوارش
سگ خانگی
شیراز
2008
06
21
163
164
https://jvr.ut.ac.ir/article_23944_5779b0ec15a2179dbe34c8b6f5d51dfe.pdf