University of Tehran Press Journal of Veterinary Research 2008-2525 56 3 2001 10 23 - - 16492 FA Journal Article 1970 01 01 Sixteen seronegative dogs were randomly divided into two groups. All of the 8 dogs of group I recived an intraperitoneal challenge of 2.5 106 infective promastigotes of L. infantum (MCAN/1R194/Moheb 1). All dogs of Group I and group 2 were tested from 1 to 5 month after challenge by ELISA and IFA techniques for detecting anti-leishmania antibodies. Necropsy was performed on all dogs to investigate for parasites. A complete correlation was observed between ELISA and parasitological procedures. Sixteen seronegative dogs were randomly divided into two groups. All of the 8 dogs of group I recived an intraperitoneal challenge of 2.5 106 infective promastigotes of L. infantum (MCAN/1R194/Moheb 1). All dogs of Group I and group 2 were tested from 1 to 5 month after challenge by ELISA and IFA techniques for detecting anti-leishmania antibodies. Necropsy was performed on all dogs to investigate for parasites. A complete correlation was observed between ELISA and parasitological procedures. https://jvr.ut.ac.ir/article_16492_76398fe1687b7a339ac8db6138e11609.pdf