University of Tehran Press Journal of Veterinary Research 2008-2525 64 2 2009 06 22 EFFECTS OF ZATARIA MULTIFLORA ESSENTIAL OIL ON RAINBOW TROUT (ONCORHYNCHUS MYKISS) EGG HATCHABILITY AND SURVIVAL OF LARVAE COMPARED WITH HYDROGEN PEROXIDE AND MALACHITE GREEN EFFECTS OF ZATARIA MULTIFLORA ESSENTIAL OIL ON RAINBOW TROUT (ONCORHYNCHUS MYKISS) EGG HATCHABILITY AND SURVIVAL OF LARVAE COMPARED WITH HYDROGEN PEROXIDE AND MALACHITE GREEN 19560 FA Mehdi Soltani Mehdi Esfandiary Suheyla Khazraeenia Mir Masood Sajadi Journal Article 1970 01 01 Zataria multiflora essential oil, hydrogen peroxide(H2o2) and malachite green (MG) were used to assess their effects on rainbow trout egg hatchability and survival of larvae up to 1-1.2 g body weight using spring water at 12.5 °C, dissovled oxygen 8-9 mg/L, carbon dioxide 7±2 mg/L, ammunia <0.01 and total hardness about 170 mg/L . Zataria was used at 70 mg/L for 30 minutes per day , at 500 mg/L for 35 minutes per day with a reducing in the time treatment to 5 minutes during 70-140 degree-day and MG as the positive control at 2mg/L for 20 minutes per day. Normal control without any treatment was also included. Each treatment trial was provided in three replicates. The treatment trials started 36 hours post-incubation and were continued until the eyed-egg stage. The obtained results showed that the mortality level in normal control was significantly higher than other groups (p<0.05). Also, the mortality rate in eggs treated with Zataria was significantly higher than both MG and H2o2 groups (p<0.05). No significant different was seen between MG and H2o2 groups until the eyed-egg stage (p>0.05). Level of mortality in egg treated with Zataria was significantly higher than MG from eyed-egg stage until the hatching stage (p<0.05). In addition, the mortality rate of the produced larvae kept to 1-1.2 g body weight was significantly higher in both Zataria group and normal control groups than other two groups (p<0.05), while no significant difference was seen in weight of the produced larvae among all examined groups (p>0.05).The results showed that use of Zataria at 70 mg/L is incomparable with H2o2 and MG , although it is able to significantly improve the survival rate of rainbow trout eggs and larvae during incubation period. Zataria multiflora essential oil, hydrogen peroxide(H2o2) and malachite green (MG) were used to assess their effects on rainbow trout egg hatchability and survival of larvae up to 1-1.2 g body weight using spring water at 12.5 °C, dissovled oxygen 8-9 mg/L, carbon dioxide 7±2 mg/L, ammunia <0.01 and total hardness about 170 mg/L . Zataria was used at 70 mg/L for 30 minutes per day , at 500 mg/L for 35 minutes per day with a reducing in the time treatment to 5 minutes during 70-140 degree-day and MG as the positive control at 2mg/L for 20 minutes per day. Normal control without any treatment was also included. Each treatment trial was provided in three replicates. The treatment trials started 36 hours post-incubation and were continued until the eyed-egg stage. The obtained results showed that the mortality level in normal control was significantly higher than other groups (p<0.05). Also, the mortality rate in eggs treated with Zataria was significantly higher than both MG and H2o2 groups (p<0.05). No significant different was seen between MG and H2o2 groups until the eyed-egg stage (p>0.05). Level of mortality in egg treated with Zataria was significantly higher than MG from eyed-egg stage until the hatching stage (p<0.05). In addition, the mortality rate of the produced larvae kept to 1-1.2 g body weight was significantly higher in both Zataria group and normal control groups than other two groups (p<0.05), while no significant difference was seen in weight of the produced larvae among all examined groups (p>0.05).The results showed that use of Zataria at 70 mg/L is incomparable with H2o2 and MG , although it is able to significantly improve the survival rate of rainbow trout eggs and larvae during incubation period. https://jvr.ut.ac.ir/article_19560_747ce14874f1c2bd6a0e612eb2d67fbd.pdf