University of Tehran Press Journal of Veterinary Research 2008-2525 66 3 2011 09 23 EFFECT OF GARLIC ESSENTIAL OIL AS AN IMMUNOSTIMULANT ON HEMATOLOGICAL INDICES OF JUVENIL BELUGA (HUSO HUSO EFFECT OF GARLIC ESSENTIAL OIL AS AN IMMUNOSTIMULANT ON HEMATOLOGICAL INDICES OF JUVENIL BELUGA (HUSO HUSO 209 216 23371 FA Ebrahim Alizadeh Doughikollaee Ebrahim Alizadeh Doghikollaee Eisa Ebrahimi Parviz Zare Journal Article 1970 01 01 Beluga (Huso huso) is one of the most valuable species of sturgeons and many efforts have been made to artificially reproduce, culture and restore this precious species. The objective of this study was to find the effects of garlic essential oil as an immunostimulant on hematological indices and on the cellular immunity system of juvenile belugas. An experiment was organized in which six different diets were fed to belugas including: diet without garlic essential oil and antibiotic, diet with oxytetracycline antibiotic (30 mg/kg), and four diets with various amounts of garlic essential oil (0.05, 0.10, 0.15 and 0.20 g/kg). Beluga juveniles with an average weight of 24 ± 2 g were fed these diets for 56 days. At the end of the experiment, hematological indices were determined. The blood coagulation time and mean corpuscular hemoglobin (MCH) indices increased in the groups treated with garlic essential oil. Significant increases in lymphocyte counts were observed in groups fed 0.05, 0.10 and 0.15 g/kg garlic essential oil as compared with the antibiotic and control groups. A significant decrease in the blood neutrophil count was observed in the belugas fed 0.15 and 0.20 g/kg garlic essential oil compared with the other treatments. In conclusion, these results showed that adding garlic essential oil increases the blood coagulation time, the hemoglobin content of red blood cells, lymphocyte numbers, and decreases eosinophils. Significant improvements of the immune system and of the physiological condition were observed in juvenile belugas receiving garlic essential oil compared with the control and the antibiotic treatments. It can therefore be used as a suitable replacement for oxytetracycline in the diet of juvenile belugas. Beluga (Huso huso) is one of the most valuable species of sturgeons and many efforts have been made to artificially reproduce, culture and restore this precious species. The objective of this study was to find the effects of garlic essential oil as an immunostimulant on hematological indices and on the cellular immunity system of juvenile belugas. An experiment was organized in which six different diets were fed to belugas including: diet without garlic essential oil and antibiotic, diet with oxytetracycline antibiotic (30 mg/kg), and four diets with various amounts of garlic essential oil (0.05, 0.10, 0.15 and 0.20 g/kg). Beluga juveniles with an average weight of 24 ± 2 g were fed these diets for 56 days. At the end of the experiment, hematological indices were determined. The blood coagulation time and mean corpuscular hemoglobin (MCH) indices increased in the groups treated with garlic essential oil. Significant increases in lymphocyte counts were observed in groups fed 0.05, 0.10 and 0.15 g/kg garlic essential oil as compared with the antibiotic and control groups. A significant decrease in the blood neutrophil count was observed in the belugas fed 0.15 and 0.20 g/kg garlic essential oil compared with the other treatments. In conclusion, these results showed that adding garlic essential oil increases the blood coagulation time, the hemoglobin content of red blood cells, lymphocyte numbers, and decreases eosinophils. Significant improvements of the immune system and of the physiological condition were observed in juvenile belugas receiving garlic essential oil compared with the control and the antibiotic treatments. It can therefore be used as a suitable replacement for oxytetracycline in the diet of juvenile belugas. https://jvr.ut.ac.ir/article_23371_ee6f53e84f1b4f825aa11965c8a93492.pdf