دانشگاه تهران مجله تحقیقات دامپزشکی (Journal of Veterinary Research) 2008-2525 62 1 2007 03 21 STUDY OF EFFECTOR ANTIGENS OF IBR VIRUS ON HUMORALIMMUNITY مطالعه پادگن های دخیل در ایمنی همورال ویروس IBR 57 61 24027 FA فرهید همت زاده گروه میکروبیولوژی و ایمونولوژی، دانشکده دامپزشکی دانشگاه تهران هادی کیوانفر گروه میکروبیولوژی و ایمونولوژی، دانشکده دامپزشکی دانشگاه تهران مهدی احمدی دانش آموخته دکتری دامپزشکی دانشکده دامپزشکی دانشگاه تهران Journal Article 1970 01 01 The objective of this study was to determine the protein pattern and antigenic structure of BHV-1. Ten viral isolates from cows with clinical signs of respiratory infections were selected for this study and confirmed by SN test. All samples were cultured on cell cultures and purified by ultracentrifugation. Purified isolates were electerophorsed using SDS-PAGE method and immunoblotted. Viral antigens were detected using anti BHV-1 bovine antiserum and molecular weight of the bands were determined. All samples showed identical band number and molecular weight except for one sample which revealed an additional 120-125 KDa band. Also immunoblotting data resulted in identified of twelve bands (150, 130, 115, 97, 77, 70, 55,45, 40, 38, 32 and 25 KDa) suggesting involvement of these antigens in evoking humoral immune response in affected cattle. The objective of this study was to determine the protein pattern and antigenic structure of BHV-1. Ten viral isolates from cows with clinical signs of respiratory infections were selected for this study and confirmed by SN test. All samples were cultured on cell cultures and purified by ultracentrifugation. Purified isolates were electerophorsed using SDS-PAGE method and immunoblotted. Viral antigens were detected using anti BHV-1 bovine antiserum and molecular weight of the bands were determined. All samples showed identical band number and molecular weight except for one sample which revealed an additional 120-125 KDa band. Also immunoblotting data resulted in identified of twelve bands (150, 130, 115, 97, 77, 70, 55, 45, 40, 38, 32 and 25 KDa) suggesting involvement of these antigens in evoking humoral immune response in affected cattle. https://jvr.ut.ac.ir/article_24027_04c1f9df23fd759ef4b121ddc8ef1d66.pdf