University of Tehran Press Journal of Veterinary Research 2008-2525 75 4 2020 12 21 Evaluation of the Effects of PRCR on Differentiation of Autologous Mesenchymal Stem Cells into Tenocytes in Rabbit Evaluation of the Effects of PRCR on Differentiation of Autologous Mesenchymal Stem Cells into Tenocytes in Rabbit 498 508 79858 10.22059/jvr.2020.250664.2754 FA MersedehSadat Hossein Boroujerdi Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Mohammad Mehdi Dehghan Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Fereydoun Saberi Afshar Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Keyvan Majidzadeh-A Motamed Institute, Jahade Daneshgahi, Tehran, Iran Seyed Hossein Mardjanmehr Department of Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Artimes Yahyaei Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Masoumeh Jabarifakhr Institute of Biomedical Research, University of Tehran, Tehran, Iran Journal Article 2020 05 16 BACKGROUND: Tendon repair requires a sequence of medical procedures such as physical modalities, tissue engineering, growth factors, and mechanical stimulation. Previous studies have shown that using of PRP is effective in tendon healing. <br />OBJECTIVES: The purpose of this study was to investigate the differentiation effect of PRCR on mesenchymal stem cells extracted from rabbits’ bone marrow. <br />METHODS: Mesenchymal stem cells were extracted from bone marrow (BM-MSCs) of 10 New Zealand white rabbits. The cells were then examined both flow cytometrically and morphologically. Afterwards, the cells received treatment using PRCR. Finally, the markers indicating tenocytes were analyzed through the method of Real-Time PCR. <br />RESULTS: Investigating the differences between differentiated cells and undifferentiated stem cells (BM-MSCs) regarding the expression of four genes including Scx, Tnmd, Col I, and Tenascin C revealed that the level of expression in all the four genes was significantly enhanced after receiving PRCR- mediated treatment. <br />CONCLUSIONS: BM-MSCs treated with PRCR express high levels of tendon-related genes and undergo differentiation into tenocyte-like cells. BACKGROUND: Tendon repair requires a sequence of medical procedures such as physical modalities, tissue engineering, growth factors, and mechanical stimulation. Previous studies have shown that using of PRP is effective in tendon healing. <br />OBJECTIVES: The purpose of this study was to investigate the differentiation effect of PRCR on mesenchymal stem cells extracted from rabbits’ bone marrow. <br />METHODS: Mesenchymal stem cells were extracted from bone marrow (BM-MSCs) of 10 New Zealand white rabbits. The cells were then examined both flow cytometrically and morphologically. Afterwards, the cells received treatment using PRCR. Finally, the markers indicating tenocytes were analyzed through the method of Real-Time PCR. <br />RESULTS: Investigating the differences between differentiated cells and undifferentiated stem cells (BM-MSCs) regarding the expression of four genes including Scx, Tnmd, Col I, and Tenascin C revealed that the level of expression in all the four genes was significantly enhanced after receiving PRCR- mediated treatment. <br />CONCLUSIONS: BM-MSCs treated with PRCR express high levels of tendon-related genes and undergo differentiation into tenocyte-like cells. https://jvr.ut.ac.ir/article_79858_bb8c7f24b0ea386db282cfe1a16462e5.pdf