University of Tehran Press Journal of Veterinary Research 2008-2525 76 1 2021 03 21 A Serological Study of Neospora caninum Infection in Dairy Cattle in Semnan Province, Iran A Serological Study of Neospora caninum Infection in Dairy Cattle in Semnan Province, Iran 1 7 81629 10.22059/jvr.2020.291705.2985 FA Marziyeh Binaei Graduated from the Faculty of Veterinary Medicine, Semnan University, Semnan, Iran Emad Changizi Department of Pathobiology, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran Hamid Staji Department of Pathobiology, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran Journal Article 2020 10 03 BACKGROUND: Over the recent years, <em>Neospora caninum</em> has been one of the most important causes of abortion in dairy cattle. <br />OBJECTIVES: We conducted the present study in order to investigate the seroprevalence of <em>N. caninum</em> in dairy cattle in Semnan province and its effect on abortion. <br />METHODS: 237 blood samples were obtained from various Semnan dairy farms and 104 bulk dairy samples from four milk collection centers in Semnan, Garmsar, Damghan, and Shahrood were tested for sera and milk utilizing ELISA (Svanova Biotech AB) test kits. <br />RESULTS: The results revealed that 87.27 % of bovine serum was positive. The percentage of opacity density (OD) of positive sample (PP) ranged from 72.17 to 137.3 (114.21±24.65). In addition, the average rate of milk seroprevalence to the parasite was 95.23 % in Semnan province. <br />CONCLUSIONS: The frequency of <em>Neospora caninum</em> infection in blood and milk was high in Semnan, yet no significant relationships were observed with abortion (<em>p < /em>>0.05).</em> BACKGROUND: Over the recent years, <em>Neospora caninum</em> has been one of the most important causes of abortion in dairy cattle. <br />OBJECTIVES: We conducted the present study in order to investigate the seroprevalence of <em>N. caninum</em> in dairy cattle in Semnan province and its effect on abortion. <br />METHODS: 237 blood samples were obtained from various Semnan dairy farms and 104 bulk dairy samples from four milk collection centers in Semnan, Garmsar, Damghan, and Shahrood were tested for sera and milk utilizing ELISA (Svanova Biotech AB) test kits. <br />RESULTS: The results revealed that 87.27 % of bovine serum was positive. The percentage of opacity density (OD) of positive sample (PP) ranged from 72.17 to 137.3 (114.21±24.65). In addition, the average rate of milk seroprevalence to the parasite was 95.23 % in Semnan province. <br />CONCLUSIONS: The frequency of <em>Neospora caninum</em> infection in blood and milk was high in Semnan, yet no significant relationships were observed with abortion (<em>p < /em>>0.05).</em> https://jvr.ut.ac.ir/article_81629_bafd9b3af0a12f618587a84c0dc53686.pdf