University of Tehran Press Journal of Veterinary Research 2008-2525 76 1 2021 03 21 Molecular Discrimination of Different Types of Trypanosoma Evansi in One-Humped Camels (Camelus dromedarius) in Sistan-va-Baluchestan Province, Iran Molecular Discrimination of Different Types of Trypanosoma Evansi in One-Humped Camels (Camelus dromedarius) in Sistan-va-Baluchestan Province, Iran 8 13 81630 10.22059/jvr.2020.275071.2901 FA Fereshte Mirshekar Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Mohammad Yakhchali Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Fariborz Shariati-Sharifi Department of Pathobiology, Faculty of Veterinary Medicine, Parasitology Division, ZabolUniversity, Zabol, Iran Journal Article 2020 08 31 BACKGROUND: Trypanosomosis is a blood parasitic disease with veterinary and cosmopolitan importance due to <em>Trypanosoma evansi </em>(Kinetoplastida: Trypanosomatidae) type A in camels, cattle, buffaloes, and equine and type B in camels. <br />OBJECTIVES: We conducted the present study to discriminate <em>Trypanosoma evansi</em> type A and B infection in one-humped camels (<em>Camelus dromedarius</em>) in Sistan-va-Baluchestan Province, south eastern Iran. <br />METHODS: A total number of 369 blood samples were randomly taken from jugular <em>vein</em> of the examined one-humped camels from different parts of the region. Genomic DNA was extracted and polymerase chain reaction (PCR) was performed to amplify 205bp-fragment-length and 436bp-fragment-length of RoTat 1.2 VSG gene (<em>T. evansi</em> type A) and Minicircle gene (<em>T. evansi</em> type B), respectively. <br />RESULTS: Molecular findings revealed that all the infected camels were affected by <em>T. evansi</em> type A. <br />CONCLUSIONS: Based on the results of the current study, we could conclude that the cause of infection in the examined camels of the region, like other parts of the world, was <em>T. evansi</em> type A. BACKGROUND: Trypanosomosis is a blood parasitic disease with veterinary and cosmopolitan importance due to <em>Trypanosoma evansi </em>(Kinetoplastida: Trypanosomatidae) type A in camels, cattle, buffaloes, and equine and type B in camels. <br />OBJECTIVES: We conducted the present study to discriminate <em>Trypanosoma evansi</em> type A and B infection in one-humped camels (<em>Camelus dromedarius</em>) in Sistan-va-Baluchestan Province, south eastern Iran. <br />METHODS: A total number of 369 blood samples were randomly taken from jugular <em>vein</em> of the examined one-humped camels from different parts of the region. Genomic DNA was extracted and polymerase chain reaction (PCR) was performed to amplify 205bp-fragment-length and 436bp-fragment-length of RoTat 1.2 VSG gene (<em>T. evansi</em> type A) and Minicircle gene (<em>T. evansi</em> type B), respectively. <br />RESULTS: Molecular findings revealed that all the infected camels were affected by <em>T. evansi</em> type A. <br />CONCLUSIONS: Based on the results of the current study, we could conclude that the cause of infection in the examined camels of the region, like other parts of the world, was <em>T. evansi</em> type A. https://jvr.ut.ac.ir/article_81630_a986eebac261692bad69d7a74a87e43c.pdf