University of Tehran Press Journal of Veterinary Research 2008-2525 81 1 2027 02 20 Molecular Investigation of Bovine Hydatid Cyst Strains from A Slaughterhouse in Zabol, Southeast of Iran Molecular Investigation of Bovine Hydatid Cyst Strains from A Slaughterhouse in Zabol, Southeast of Iran 1 11 105936 10.22059/jvr.2025.385638.3467 FA Nahid Ozbakzaee Graduated from the Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran 0009-0004-0940-0200 Maryam Ganjali Department of Pathobiology, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran Fereshteh Mirshekar Member of Young and Elite Researchers Club, Zabol Branch, Islamic Azad University, Zabol, Zabol, Iran Journal Article 2025 10 22 BACKGROUND: Hydatid cyst, caused by the larval stage of the cestode Echinococcus granulosus (E. granulosus) is one of the most significant zoonotic diseases and a major global public health concern. In endemic regions, this parasite exhibits substantial genetic diversity in its biological characteristics. To date, 10 distinct genotypes of E. granulosus (G1–G10) have been identified. Studies worldwide demonstrate that the genotypic variation and complex nature of E. granulosus influence the parasite’s life cycle, transmission pathways, pathogenicity, antigenicity, immunogenicity, drug response, epidemiology, and disease control strategies. OBJECTIVES: This study aimed to perform a molecular characterization of bovine hydatid cyst strains collected from a slaughterhouse in Zabol, south of Iran. METHODS: This study was conducted in the autumn and winter of 2022 on 50 samples with a definitive diagnosis of hydatid cyst collected from a slaughterhouse in Zabol city. DNA was extracted from protoscolices and germinal layers, followed by PCR amplification of the ITS2 gene. The PCR products were then digested with the restriction enzyme HpaII and the samples were sequenced. RESULTS: The PCR product ITS2 was 750 bp in size and was digested with HpaII restriction enzymes. According to the Restriction fragment length polymorphism analysis, the isolates belonged to a single species named E. granulosus sensu stricto (G1–G3 complex) and sequencing also confirmed the results. CONCLUSIONS: Based on the determination of parasite genotypes in the region, a sheep transmission pattern is proposed, which can provide the appropriate prevention pattern according to the life cycle of the parasite. BACKGROUND: Hydatid cyst, caused by the larval stage of the cestode Echinococcus granulosus (E. granulosus) is one of the most significant zoonotic diseases and a major global public health concern. In endemic regions, this parasite exhibits substantial genetic diversity in its biological characteristics. To date, 10 distinct genotypes of E. granulosus (G1–G10) have been identified. Studies worldwide demonstrate that the genotypic variation and complex nature of E. granulosus influence the parasite’s life cycle, transmission pathways, pathogenicity, antigenicity, immunogenicity, drug response, epidemiology, and disease control strategies. OBJECTIVES: This study aimed to perform a molecular characterization of bovine hydatid cyst strains collected from a slaughterhouse in Zabol, south of Iran. METHODS: This study was conducted in the autumn and winter of 2022 on 50 samples with a definitive diagnosis of hydatid cyst collected from a slaughterhouse in Zabol city. DNA was extracted from protoscolices and germinal layers, followed by PCR amplification of the ITS2 gene. The PCR products were then digested with the restriction enzyme HpaII and the samples were sequenced. RESULTS: The PCR product ITS2 was 750 bp in size and was digested with HpaII restriction enzymes. According to the Restriction fragment length polymorphism analysis, the isolates belonged to a single species named E. granulosus sensu stricto (G1–G3 complex) and sequencing also confirmed the results. CONCLUSIONS: Based on the determination of parasite genotypes in the region, a sheep transmission pattern is proposed, which can provide the appropriate prevention pattern according to the life cycle of the parasite.

https://jvr.ut.ac.ir/article_105936_074a99a061a739c52a4eb51a54b59ac9.pdf

University of Tehran Press Journal of Veterinary Research 2008-2525 81 1 2026 03 21 Isolation, Characterization, and Biocontrol Efficacy of Bacteriophage VMUT_SIR1 Against Salmonella enteritidis in Chicken Meat Isolation, Characterization, and Biocontrol Efficacy of Bacteriophage VMUT_SIR1 Against Salmonella enteritidis in Chicken Meat 13 29 105295 10.22059/jvr.2025.400880.3536 FA Reza Teimouri Fard Graduated from the Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Afshin Akhondzadeh Basti Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Ali Khanjari Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Negin Noori Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Hoseinali Ebrahimizadeh Mousavi Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Iradj Ashrafi Tamai Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Elnaz Kamrani Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Journal Article 2025 10 11 BACKGROUND: Salmonella enteritidis (S. enteritidis) is one of the most prevalent bacterial pathogens responsible for foodborne illnesses transmitted through chicken meat. Given the escalating threats posed by antibiotic resistance in this bacterium, the application of bacteriophages as modern biocontrol agents has gained significant importance. OBJECTIVES: The present study aimed to isolate and characterize indigenous bacteriophages effective against S. enteritidis from wastewater of industrial poultry slaughterhouses and to evaluate their efficacy in controlling Salmonella contamination in chicken meat. METHODS: Twenty 50-mL samples of wastewater were collected from an industrial poultry slaughterhouse in Tehran, Iran, for phage isolation. Purification and propagation of the bacteriophage were performed using the double-layer agar method and plaque assay. The isolated phage was characterized in terms of morphology, host range, lytic activity, multiplicity of infection (MOI), adsorption efficiency, one-step growth curve, stability under various temperature and pH conditions, as well as preventive and corrective treatments on chicken meat. RESULTS: The purified bacteriophage belonged to the Siphoviridae family and exhibited a broad host range of 90% against S. enteritidis serovars. The highest lytic activity and optimal bacteriophage efficacy against the bacterium were observed at an MOI of 0.01. The maximum adsorption percentage occurred within the initial 8 minutes, reaching a peak of 92% at 12 minutes. The phage VMUT_SIR1 demonstrated stability at a temperature range of -18°C to 70°C and pH values of 4 to 12. In treatments applied to chicken meat, the optimal MOI of the bacteriophage yielded the best results in corrective treatment at 25°C, achieving a 2.88 Log CFU/g reduction within the first 6 hours, and a 2.4 Log CFU/g reduction over 24 hours in corrective treatment with the phage at 4°C. CONCLUSIONS: The bacteriophage VMUT_SIR1 exhibited substantial potential as a biological disinfectant for controlling Salmonella in chicken meat production in the conducted study. BACKGROUND: Salmonella enteritidis (S. enteritidis) is one of the most prevalent bacterial pathogens responsible for foodborne illnesses transmitted through chicken meat. Given the escalating threats posed by antibiotic resistance in this bacterium, the application of bacteriophages as modern biocontrol agents has gained significant importance. OBJECTIVES: The present study aimed to isolate and characterize indigenous bacteriophages effective against S. enteritidis from wastewater of industrial poultry slaughterhouses and to evaluate their efficacy in controlling Salmonella contamination in chicken meat. METHODS: Twenty 50-mL samples of wastewater were collected from an industrial poultry slaughterhouse in Tehran, Iran, for phage isolation. Purification and propagation of the bacteriophage were performed using the double-layer agar method and plaque assay. The isolated phage was characterized in terms of morphology, host range, lytic activity, multiplicity of infection (MOI), adsorption efficiency, one-step growth curve, stability under various temperature and pH conditions, as well as preventive and corrective treatments on chicken meat. RESULTS: The purified bacteriophage belonged to the Siphoviridae family and exhibited a broad host range of 90% against S. enteritidis serovars. The highest lytic activity and optimal bacteriophage efficacy against the bacterium were observed at an MOI of 0.01. The maximum adsorption percentage occurred within the initial 8 minutes, reaching a peak of 92% at 12 minutes. The phage VMUT_SIR1 demonstrated stability at a temperature range of -18°C to 70°C and pH values of 4 to 12. In treatments applied to chicken meat, the optimal MOI of the bacteriophage yielded the best results in corrective treatment at 25°C, achieving a 2.88 Log CFU/g reduction within the first 6 hours, and a 2.4 Log CFU/g reduction over 24 hours in corrective treatment with the phage at 4°C. CONCLUSIONS: The bacteriophage VMUT_SIR1 exhibited substantial potential as a biological disinfectant for controlling Salmonella in chicken meat production in the conducted study.

https://jvr.ut.ac.ir/article_105295_2279f82a002454d04b18c1322a68776a.pdf

University of Tehran Press Journal of Veterinary Research 2008-2525 81 1 2026 03 21 Comparing the Effects of Injectable Mineral and Vitamin Supplements on Oxidative Stress Indices During the Transition Period in Holstein Cows Comparing the Effects of Injectable Mineral and Vitamin Supplements on Oxidative Stress Indices During the Transition Period in Holstein Cows 31 41 105935 10.22059/jvr.2025.391841.3496 FA Mohammad Asadi Graduated from the Agricultural and Natural Resources Research and Education Center of Gorgan, Gorgan, Iran Reza Kamali Department of Animal Sciences Research, Agricultural and Natural Resources Research and Education Center of Gorgan, Gorgan, Iran Nader Asadzadeh Scientific Board Member of Animal Science Research Institute of Iran, Agricultural Research Education and Extension Organization (AREEO) Iran Journal Article 2025 10 26 BACKGROUND: One of the most important and stressful periods in a dairy cow's life is the transition period (3 weeks before and 3 weeks after calving). Mineral and vitamin supplements have beneficial effects on controlling the negative effects of oxidative stress during the transition period in dairy cows. OBJECTIVES: This study aimed to compare the effects of mineral and vitamin supplement injections on oxidative stress indices during the transition period of Holstein cows. METHODS: In this study, 32 cows in late pregnancy were randomly divided into four groups: Control group (no vitamin and mineral supplement injection), treatment group 1, receiving injectable vitamin supplements (A, D3, E, B1, B2, B3, B6, B5, B12 and C), treatment group 2, receiving injectable mineral supplement (calcium, magnesium, copper and phosphorus elements), and treatment group 3, receiving both injectable vitamin and mineral supplements. Injection was performed subcutaneously 28 days before calving. Blood sampling was performed to investigate the effect of mineral and vitamin supplements on inflammatory indices and oxidative stress 21 days before delivery, on the day of calving, and 21 days after delivery. RESULTS: The results showed that the treatment groups showed a significant decrease in beta-hydroxybutyrate, non-esterified fatty acids, reactive oxygen species, and oxidative stress index compared to the control group (P<0.05). Furthermore, the three treatment groups demonstrated a significant increase in the serum antioxidant capacity of dairy cows compared to the control group (P< 0.05). The effect of time on the concentration of NEFA, BHB, ROS, SAC, and OSI in different treatment groups was significant throughout the entire period (P<0.05). The interaction effect of group and time on the measured parameters was also significant (P<0.05). CONCLUSIONS: The simultaneous injection of mineral and vitamin supplements is recommended for dairy cows in the prenatal transition period due to the reduction of inflammation and oxidative stress. BACKGROUND: One of the most important and stressful periods in a dairy cow's life is the transition period (3 weeks before and 3 weeks after calving). Mineral and vitamin supplements have beneficial effects on controlling the negative effects of oxidative stress during the transition period in dairy cows. OBJECTIVES: This study aimed to compare the effects of mineral and vitamin supplement injections on oxidative stress indices during the transition period of Holstein cows. METHODS: In this study, 32 cows in late pregnancy were randomly divided into four groups: Control group (no vitamin and mineral supplement injection), treatment group 1, receiving injectable vitamin supplements (A, D3, E, B1, B2, B3, B6, B5, B12 and C), treatment group 2, receiving injectable mineral supplement (calcium, magnesium, copper and phosphorus elements), and treatment group 3, receiving both injectable vitamin and mineral supplements. Injection was performed subcutaneously 28 days before calving. Blood sampling was performed to investigate the effect of mineral and vitamin supplements on inflammatory indices and oxidative stress 21 days before delivery, on the day of calving, and 21 days after delivery. RESULTS: The results showed that the treatment groups showed a significant decrease in beta-hydroxybutyrate, non-esterified fatty acids, reactive oxygen species, and oxidative stress index compared to the control group (P<0.05). Furthermore, the three treatment groups demonstrated a significant increase in the serum antioxidant capacity of dairy cows compared to the control group (P< 0.05). The effect of time on the concentration of NEFA, BHB, ROS, SAC, and OSI in different treatment groups was significant throughout the entire period (P<0.05). The interaction effect of group and time on the measured parameters was also significant (P<0.05). CONCLUSIONS: The simultaneous injection of mineral and vitamin supplements is recommended for dairy cows in the prenatal transition period due to the reduction of inflammation and oxidative stress.

https://jvr.ut.ac.ir/article_105935_cbdbefa421a2c3ada99c5f4dbe8fc140.pdf

University of Tehran Press Journal of Veterinary Research 2008-2525 81 1 2026 03 21 Effects of Guanidinoacetic Acid on Sperm Characteristics of Older Roosters and Hatchability Rate in Broiler Breeder Flocks Effects of Guanidinoacetic Acid on Sperm Characteristics of Older Roosters and Hatchability Rate in Broiler Breeder Flocks 43 53 105937 10.22059/jvr.2025.393823.3503 FA Hoda Javaheri Barfourooshi Department of Animal Production Management, Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran Akbar Yaghobfar Department of Poultry Nutrition Animal Science Research Institute (ASRI), Agricultural Research, Education, and Extension Organization (AREEO), Karaj, Iran Journal Article 2025 10 15 BACKGROUND: Physical decline in older roosters negatively affects their mating behavior and hatchability in broiler breeder flocks. Energy-producing compounds such as guanidinoacetic acid (GAA) may help address this issue. OBJECTIVES: This study aimed to evaluate the effects of different levels of GAA on sperm characteristics and testicular histology in older roosters, and hatchability rate in a broiler breeder flock. METHODS: A total of 144 hens and 18 roosters from a Ross 308 breeder flock were divided into three groups; one control group feeding based on the Ross 308 feeding guides, a treatment group receiving 1200 mg/kg of GAA (CreAmino), and a treatment group receiving 1600 mg/kg of CreAmino. Each group consisted of 48 hens and 8 roosters, with 6 replicates per treatment. Data were recorded done for 20 weeks, starting from week 41. The data included body weight, testicular weight, quantitative and qualitative sperm characteristics, serum concentrations of creatinine, nitric oxide, insulin, and testosterone, as well as histological testicular parameters and egg hatchability rates. RESULTS: No significant differences in sperm characteristics were found among the groups. However, both CreAmino treatment groups showed a significant increase in the number of spermatogonia, spermatocytes, spermatids, and Leydig cells, as well as an increase in the diameter of the seminiferous tubules and the thickness of the germinal epithelium, compared to the control group (P≤0.05). Serum keratin levels were significantly elevated in the CreAmino 1200 group during the second sampling period compared to the CreAmino 1600 and control groups (P≤0.05). Furthermore, serum nitric oxide concentrations were significantly higher in the CreAmino 1600 group during the second sampling period and in the control group during the third sampling period, compared to other two groups (P≤0.05). The hatchability rates were not significantly different among the groups. CONCLUSIONS: The use of 1200 mg CreAmino can improve both quantitative and qualitative sperm characteristics and histological parameters in older roosters. However, the treatment with GAA does not significantly affect hatchability rate in broiler breeder flocks. BACKGROUND: Physical decline in older roosters negatively affects their mating behavior and hatchability in broiler breeder flocks. Energy-producing compounds such as guanidinoacetic acid (GAA) may help address this issue. OBJECTIVES: This study aimed to evaluate the effects of different levels of GAA on sperm characteristics and testicular histology in older roosters, and hatchability rate in a broiler breeder flock. METHODS: A total of 144 hens and 18 roosters from a Ross 308 breeder flock were divided into three groups; one control group feeding based on the Ross 308 feeding guides, a treatment group receiving 1200 mg/kg of GAA (CreAmino), and a treatment group receiving 1600 mg/kg of CreAmino. Each group consisted of 48 hens and 8 roosters, with 6 replicates per treatment. Data were recorded done for 20 weeks, starting from week 41. The data included body weight, testicular weight, quantitative and qualitative sperm characteristics, serum concentrations of creatinine, nitric oxide, insulin, and testosterone, as well as histological testicular parameters and egg hatchability rates. RESULTS: No significant differences in sperm characteristics were found among the groups. However, both CreAmino treatment groups showed a significant increase in the number of spermatogonia, spermatocytes, spermatids, and Leydig cells, as well as an increase in the diameter of the seminiferous tubules and the thickness of the germinal epithelium, compared to the control group (P≤0.05). Serum keratin levels were significantly elevated in the CreAmino 1200 group during the second sampling period compared to the CreAmino 1600 and control groups (P≤0.05). Furthermore, serum nitric oxide concentrations were significantly higher in the CreAmino 1600 group during the second sampling period and in the control group during the third sampling period, compared to other two groups (P≤0.05). The hatchability rates were not significantly different among the groups. CONCLUSIONS: The use of 1200 mg CreAmino can improve both quantitative and qualitative sperm characteristics and histological parameters in older roosters. However, the treatment with GAA does not significantly affect hatchability rate in broiler breeder flocks.

https://jvr.ut.ac.ir/article_105937_4d10c39d155b28bbb6563faf8b73fef4.pdf

University of Tehran Press Journal of Veterinary Research 2008-2525 81 1 2026 03 21 Molecular Isolation of Coagulase-Negative Staphylococci From Milk of Cows With Mastitis in Dairy Farms in Karaj City, Iran, During 2023 Molecular Isolation of Coagulase-Negative Staphylococci From Milk of Cows With Mastitis in Dairy Farms in Karaj City, Iran, During 2023 55 71 105541 10.22059/jvr.2025.394736.3506 FA Fateh Rahimi Department of Cell and Molecular Biology & Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran Sanaz Khashei Graduated from the Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Journal Article 2025 10 08 BACKGROUND: Although coagulase-negative Staphylococci (CoNS) are among the predominant pathogens causing bovine mastitis, limited data are available on CoNS associated with infections in Iran. OBJECTIVES: This study aimed to determine the prevalence, antibiotic resistance patterns, and virulence-associated genes among various CoNS species isolated from bovine milk samples in Karaj City, Iran, during 2023. METHODS: Over 6 months, 318 milk samples were collected from 3 selected dairy in the suburb of Karaj, Iran. CoNS isolates were identified to the species level using biochemical tests and polymerase chain reaction (PCR). Antimicrobial susceptibility to 14 antibiotics was assessed, and the presence of the mecA and mecC genes was investigated in methicillin-resistant isolates. Additionally, the presence of virulence-associated genes, including enterotoxins (sea–seq), hemolysins (hla, hlb, and hld), tsst, and pvl was examined in all confirmed isolates. RESULTS: A total of 125 CoNS isolates were recovered and classified into 7 species: Staphylococcus epidermidis (39%), S. sciuri (16%), S. simulans (11%), S. warneri (10%), S. haemolyticus (9%), S. chromogenes (8%), and S. capitis (7%). Methicillin resistance, associated with the mecA gene, was identified in 55% of the isolates. The highest antibiotic resistance rates were observed against penicillin (84%), erythromycin (79%), and ciprofloxacin (77%). The virulence genes sea, sec, seg, seh, sek, sem, sen, seo, sep, seq, hla, hlb, and pvl were detected in 36%, 10%, 17%, 4%, 34%, 10%, 8%, 6%, 3%, 36%, 10%, 13%, and 3% of the isolates, respectively. CONCLUSIONS: The findings of this study highlight the significant role of CoNS species in bovine mastitis and underscore the need for effective infection control policies and the prudent use of appropriate antibiotics to successfully treat infections caused by CoNS. BACKGROUND: Although coagulase-negative Staphylococci (CoNS) are among the predominant pathogens causing bovine mastitis, limited data are available on CoNS associated with infections in Iran. OBJECTIVES: This study aimed to determine the prevalence, antibiotic resistance patterns, and virulence-associated genes among various CoNS species isolated from bovine milk samples in Karaj City, Iran, during 2023. METHODS: Over 6 months, 318 milk samples were collected from 3 selected dairy in the suburb of Karaj, Iran. CoNS isolates were identified to the species level using biochemical tests and polymerase chain reaction (PCR). Antimicrobial susceptibility to 14 antibiotics was assessed, and the presence of the mecA and mecC genes was investigated in methicillin-resistant isolates. Additionally, the presence of virulence-associated genes, including enterotoxins (sea–seq), hemolysins (hla, hlb, and hld), tsst, and pvl was examined in all confirmed isolates. RESULTS: A total of 125 CoNS isolates were recovered and classified into 7 species: Staphylococcus epidermidis (39%), S. sciuri (16%), S. simulans (11%), S. warneri (10%), S. haemolyticus (9%), S. chromogenes (8%), and S. capitis (7%). Methicillin resistance, associated with the mecA gene, was identified in 55% of the isolates. The highest antibiotic resistance rates were observed against penicillin (84%), erythromycin (79%), and ciprofloxacin (77%). The virulence genes sea, sec, seg, seh, sek, sem, sen, seo, sep, seq, hla, hlb, and pvl were detected in 36%, 10%, 17%, 4%, 34%, 10%, 8%, 6%, 3%, 36%, 10%, 13%, and 3% of the isolates, respectively. CONCLUSIONS: The findings of this study highlight the significant role of CoNS species in bovine mastitis and underscore the need for effective infection control policies and the prudent use of appropriate antibiotics to successfully treat infections caused by CoNS.

https://jvr.ut.ac.ir/article_105541_8897d94e41f80ac5804b3445cbca1d5e.pdf

University of Tehran Press Journal of Veterinary Research 2008-2525 81 1 2026 03 21 Occurrence of Osteopetrosis in an Adult White Leghorn Rooster: A Case Report Occurrence of Osteopetrosis in an Adult White Leghorn Rooster: A Case Report 73 77 105938 10.22059/jvr.2025.395703.3514 FA Mohammad Javad Gharagozlou Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Anahita Gharagozlou Graduated from the Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Fatemeh Moradalian Graduated from the Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Hamid Nafe Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran 0009-0006-1982-7827 Alireza Vajhi Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Farzin Bengar Graduated from the Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Journal Article 2025 09 29 Osteopetrosis, or “marble bone disease”, is a rare condition that causes bones to grow abnormally and become overly dense. Genetic disorders and/or viral infection contribute to the development of this bone disease. Osteopetrosis can result in a defect in the formation or activity of osteoclasts or an overgrowth of osteoblasts and osteocytes. Osteopetrosis has been reported in humans and different animals, including cows, dogs, cats, rabbits, mice, and birds. This study reports a case of osteopetrosis in a white leghorn rooster. The case was an 18-20-week-old white Leghorn rooster with clinical manifestations of relative anemia in unfeathered regions, walking with a stilted gait, and a “boot-like” appearance of tarsal and metatarsal bones. Bone lesions in macroscopic pathology observations and bilateral radiological images were symmetrical, with bone tissue growth inclined towards the center and a spindle-shaped appearance in some long bones. In addition, due to the growth of bone tissue towards the center, the cavity and bone marrow space were small or completely obliterated, which could explain the anemia caused by osteopetrosis. Based on the history of clinical manifestations, macroscopic and radiological findings, advanced osteopetrosis was diagnosed in an adult white leghorn rooster. Osteopetrosis, or “marble bone disease”, is a rare condition that causes bones to grow abnormally and become overly dense. Genetic disorders and/or viral infection contribute to the development of this bone disease. Osteopetrosis can result in a defect in the formation or activity of osteoclasts or an overgrowth of osteoblasts and osteocytes. Osteopetrosis has been reported in humans and different animals, including cows, dogs, cats, rabbits, mice, and birds. This study reports a case of osteopetrosis in a white leghorn rooster. The case was an 18-20-week-old white Leghorn rooster with clinical manifestations of relative anemia in unfeathered regions, walking with a stilted gait, and a “boot-like” appearance of tarsal and metatarsal bones. Bone lesions in macroscopic pathology observations and bilateral radiological images were symmetrical, with bone tissue growth inclined towards the center and a spindle-shaped appearance in some long bones. In addition, due to the growth of bone tissue towards the center, the cavity and bone marrow space were small or completely obliterated, which could explain the anemia caused by osteopetrosis. Based on the history of clinical manifestations, macroscopic and radiological findings, advanced osteopetrosis was diagnosed in an adult white leghorn rooster.

https://jvr.ut.ac.ir/article_105938_12b7af7f33babcb03c9a8bdf80f85ab2.pdf