دانشگاه تهران مجله تحقیقات دامپزشکی (Journal of Veterinary Research) 2008-2525 63 2 2008 06 21 STUDY ON DISTRIBUTION OF INFECTIOUS HEMATOPOIETIC NECROSIS (IHN) IN FIVE MAJOR PROVINCES PRODUCINGRAINBOWTROUT (ONCORHYNCHUS MYKISS) FRYIN IRAN BYINDIRECT FLUORESCENCE ANTIBODY (IFAT) AND NESTED-RTPCR TECHNIQUES مطالعه پراکنش بیماری نکروز عفونی بافت های خونساز(IHN) در پنج استان عمده تولید کننده بچه ماهی قزل آلای رنگین کمان کشور با استفاده از تکنیک های آنتی بادی درخشان به روش غیر مستقیم (IFAT) و واکنش زنجیره ای پلی مراز (nested-RT-PCR) 99 105 23933 FA مهدی سلطانی گروه بهداشت و بیماریهای آبزیان دانشکده دامپزشکی دانشگاه تهران مهدی سلطانی گروه بهداشت و بیماریهای آبزیان دانشکده دامپزشکی دانشگاه تهران فرهید همت زاده گروه میکروبیولوژی دانشکده دامپزشکی دانشگاه تهران بهرام کاظمی مرکز بیوتکنولوژی زیستی دانشگاه علوم پزشکی دانشگاه شهید بهشتی حسینعلی ابراهیم زاده موسوی گروه بهداشت و بیماریهای آبزیان دانشکده دامپزشکی دانشگاه تهران Journal Article 1970 01 01 Distribution of infectious hematopoietic necrosis (IHN) was studied in five major provinces producing rainbow trout(Oncorhynchus mykiss)fry in Iran by indirect fluorescence antibody IFATand nested-RT-PCR techniques. Also the effect of time duration was examined on some positive samples after 8 months post sampling. Samples of kidney, liver and spleen of rainbow trout fries collected from 27 trout farms were processed and examinated. Fourteen trout hatcheris located in all provinces were identified to be contaminated with IHNV tested by both techniques. The obtained results, also showed that nested-RT-PCR is more sensitive than IFATtest particularly if samples are stored for a longer time. شناسایی مراکزتکثیر آلوده به ویروس بیماری نکروز عفونی بافت های خونساز(IHN) در پنج استان عمده تولید کننده تخم چشم زده و بچه ماهی قزل آلای رنگین کمان کشور شامل مازندران، چهارمحال و بختیاری ، کهگیلویه و بویر احمد ، لرستان و فارس با استفاده از تکنیک های IFATو nested-RT-PCR و مقایسه این دو تکنیک با یکدیگر مورد مطالعه قرار گرفته است. بافت های کلیه، کبد و طحال بچه ماهی قزل آلای رنگین کمان طی شرایط استریل در محل مزارع جداسازی و نهایتاً در فریزر 70- درجه سانتیگراد نگهداری شد. به منظور افزایش اختصاصیت واکنش RT-PCR ، یک واکنش ثانویه nested-PCR روی محصول اولیه انجام گرفت. پس از هشت ماه آزمایش های فوق الذکر مجدداً روی نمونه های مثبت این مزارع تکرار شد تا حساسیت این دو روش در طی زمان برای نمونه های قدیمی مشخص گردد. نتایج حاصله نشان داد که تمامی پنج استان یاد شده آلوده به ویروس IHN هستند ( چهار مزرعه از بیست و هفت مزرعه نمونه برداری شده). نتایج بدست امده نشان داد که هر چند IFAT حساسیت قابل قبولی برای شناسایی IHNV در مراحل اول آلودگی از خود نشان می دهد با این وجود آزمایش nested-RT-PCR واجد توانایی بالاتر از RT-PCR و حساسیت بالاتری نسبت به HFAT بخصوص برای نمونه های نگهداری شده در فریزر به مدت چندین ماه می باشد. بیماری نکروز عفونی بافت های خونساز قزل آلای رنگین کمان https://jvr.ut.ac.ir/article_23933_400ec1803e0c5f2a587e4406a4becb28.pdf
دانشگاه تهران مجله تحقیقات دامپزشکی (Journal of Veterinary Research) 2008-2525 63 2 2008 06 21 EFFICACY OF USING GnRH ANTAGONIST AS PRETREATMENT ON SUPEROVULATION IN GOATS بررسی کارآیی درمان مقدماتی با آنتاگونیست GnRH در سوپراوولاسیون بز 107 111 23934 FA فرید حیدری دزفولی پژوهشکده ملی مهندسی ژنتیک و بیوتکنولوژی فرامرز قراگوزلو گروه علوم درمانگاهی دانشکده دامپزشکی دانشگاه تهران مهدی وجگانی گروه علوم درمانگاهی دانشکده دامپزشکی دانشگاه تهران سید مرتضی میرترابی بخش انتقال جنین موسسه اصلاح نژاد دام کرج مرتضی دلیری پژوهشکده ملی مهندسی ژنتیک و بیوتکنولوژی Journal Article 1970 01 01 The effect of short term usage of GnRH antagonist as pretreatment on superovulation in goats was evaluated. Statistical analysis was performed using t-test and showed that usage of GnRH antagonist as pretreatment can improve number of recovered embryos (p<0.05), but there were no statistically difference between treatment group versus control group in term of number of corpura lutea and cystic follicles(p>0.05). This study showed that usage of GnRH antagonist can improve superovulatory response in term of embryo recovery in goats .This study showed that usage of GnRH antagonist can improve superovulatory response in term of embryo recovery in goats . تاثیر استفاده کوتاه مدت از آنتاگونیست GnRH به عنوان تیمار مقدماتی در برنامه سوپراوولاسیون بز مورد ارزیابی قرار گرفت. آنالیز یافته ها با ازمون t-test نشان داد که تیمار مقدماتی با آنتاگونیست GnRH باعث بهبود تعداد جنین های استحصالی می شود( 05/0>P) ولی اختلاف معنی داری از نظر تعداد اجسام زرد و فولیکول های کیستی بین دو گروه درمان و کنترل مشاهده نشد ( 05/0<P). این تحقیق نشان داد که استفاده از آنتاگونیست GnRH به عنوان تیمار مقدماتی می تواند باعث بهبود پاسخ سوپراوولاسیون و افزایش تعداد جنین های استحصالی در برنامه سوپراوولاسیون بز شود. این تحقیق نشان داد که استفاده از آنتاگونیست GnRH به عنوان تیمار مقدماتی می تواند باعث بهبود پاسخ سوپراوولاسیون و افزایش تعداد جنین های استحصالی در برنامه سوپراوولاسیون بز شود. آنتاگونیست GnRH اجسام زرد بز جنین استحصالی سوپراوولاسیون https://jvr.ut.ac.ir/article_23934_5b1384db2be7d02d7235a5b4aa06b4a3.pdf
دانشگاه تهران مجله تحقیقات دامپزشکی (Journal of Veterinary Research) 2008-2525 63 2 2008 06 21 EFFECTS OF GAMMAIRRADIATION AND FROZEN STORAGE ON MICROBIAL, CHEMICALAND SENSORY QUALITYOFFISH FILLET تاثیر پرتودهی با اشعه گاما و نگهداری در انجماد بر روی خواص حسی، شیمیایی و باکتریایی گوشت ماهی 113 115 23935 FA علیرضا صفاریان گروه بهداشت و کنترل مواد غذایی دانشکده دامپزشکی دانشگاه تهران نوردهر رکنی گروه بهداشت و کنترل مواد غذایی دانشکده دامپزشکی دانشگاه تهران افشین آخوند زاده بستی گروه بهداشت و کنترل مواد غذایی دانشکده دامپزشکی دانشگاه تهران علیرضا باهنر گروه بهداشت و کنترل مواد غذایی دانشکده دامپزشکی دانشگاه تهران حسینعلی ابراهیم زاده موسوی گروه بهداشت و بیماری های آبزیان دانشکده دامپزشکی دانشگاه تهران نگین نوری گروه بهداشت و کنترل مواد غذایی دانشکده دامپزشکی دانشگاه تهران Journal Article 1970 01 01 In this study the effects of gamma irradiation and frozen storage on sensory, chemical and microbial quality of fish fillets was investigated. Hypophtalmyctis molitrix were equally divided to 12 fillests, then these 12 fillets were equally arranged in 4 groups (1-4). One group considered as control with no treatment and the other 3 groups was treated by 0.75, 3 and 5 KGy of gamma irradiation, respectively. All of the groups were kept in -18?c for 3 month. All of the samples were examined for microbial, chemical and sensory examinations at day zero and 3 months later. The data were analysed by ANOVA (Kruskal-Wallis) using SPSS 10. The results showed treatment with 0.75 KGy of gamma irradiation plus frozen storage in extension of shelflife of fish fillets without any unacceptable effects on sensory and chemical characteristics of them. In this study the effects of gamma irradiation and frozen storage on sensory, chemical and microbial quality of fish fillets was investigated. Hypophtalmyctis molitrix were equally divided to 12 fillests, then these 12 fillets were equally arranged in 4 groups (1-4). One group considered as control with no treatment and the other 3 groups was treated by 0.75, 3 and 5 KGy of gamma irradiation, respectively. All of the groups were kept in -18?c for 3 month. All of the samples were examined for microbial, chemical and sensory examinations at day zero and 3 months later. The data were analysed by ANOVA (Kruskal-Wallis) using SPSS 10. The results showed treatment with 0.75 KGy of gamma irradiation plus frozen storage in extension of shelflife of fish fillets without any unacceptable effects on sensory and chemical characteristics of them. اشعه گاما انجماد گوشت ماهی پرتو دهی مدت زمان نگهداری https://jvr.ut.ac.ir/article_23935_4b48eb243bdf0e82f474c7cbcd13bc8b.pdf
دانشگاه تهران مجله تحقیقات دامپزشکی (Journal of Veterinary Research) 2008-2525 63 2 2008 06 21 HISTOZOIC DEVELOPMENTAL STAGES OF MYXOBOLUS KARUNI AND MYXOBOLUS PERSICUS AND INTRODUCE THREE NEWHOSTS مطالعه مراحل رشد درون بافتی انگل های میکسوبولوس کارونی و میکسوبولوس پرسیکوس و معرفی سه میزبان جدید 117 122 23936 FA محمود معصومیان بخش بهداشت و بیماری های آبزیان، موسسه تحقیقات شیلات ایران مهدی چوبچیان دانشکده دامپزشکی ، واحد علوم و تحقیقات دانشگاه آزاد اسلامی تهران جمیله پازوکی دانشکده علوم زیستی، دانشگاه شهید بهشتی تهران عیسی شریف پور بخش بهداشت و بیماری های آبزیان، موسسه تحقیقات شیلات ایران بهیار جلالی دانشکده دامپزشکی ، واحد علوم و تحقیقات دانشگاه آزاد اسلامی تهران Journal Article 1970 01 01 Survey on developmental stages of Myxobolus karuni and M. persicus in the gills of Barboid fishes from Mezopotamian part of Iran was done. A total 296 fish specimences were examined. Random sampling was carried out during 2002- 2004 from Karoun, Karkheh Rivers and Shadgan Lagoon. The fishes transported alive to the laboratory, their gills were fixed and stained. Myxobolus karuni developed histozoic, the plasmodia were found inside the blood vessel, it developed in the epithelial cells of primery filaments, the spores are large and ellipsoidal in shape. M.persicus starts and finished the plasmodia in epithelial and endothelial of the secondary filaments, the spores are oval in shape. Up to now, the location of infection of few species of gills Myxobolus Spp. are known. According the results of this study Barbus pectoralis, B. barbulus and B. esocinus are new hosts for Myxobolus karuni and M persicus. Survey on developmental stages of Myxobolus karuni and M. persicus in the gills of Barboid fishes from Mezopotamian part of Iran was done. A total 296 fish specimences were examined. Random sampling was carried out during 2002- 2004 from Karoun, Karkheh Rivers and Shadgan Lagoon. The fishes transported alive to the laboratory, their gills were fixed and stained. Myxobolus karuni developed histozoic, the plasmodia were found inside the blood vessel, it developed in the epithelial cells of primery filaments, the spores are large and ellipsoidal in shape. M.persicus starts and finished the plasmodia in epithelial and endothelial of the secondary filaments, the spores are oval in shape. Up to now, the location of infection of few species of gills Myxobolus Spp. are known. According the results of this study Barbus pectoralis, B. barbulus and B. esocinus are new hosts for Myxobolus karuni and M persicus. آبشش ایران میزبان های جدید میکسوبولوس پرسیکوس میکسوبولوس کارونی https://jvr.ut.ac.ir/article_23936_59784aa19a528af06b13b0038aca02bd.pdf
دانشگاه تهران مجله تحقیقات دامپزشکی (Journal of Veterinary Research) 2008-2525 63 2 2008 06 21 IDENTIFICATION OF TICK SPECIES OF DERMACENTOR IN SOME LOCALITIES OF IRAN تعیین گونه های کنه در ماسنتور در برخی از مناطق ایران 123 126 23937 FA صدیقه نبیان گروه انگل شناسی دانشکده دامپزشکی دانشگاه تهران 0000-0002-2350-4151 صادق رهبری گروه انگل شناسی دانشکده دامپزشکی دانشگاه تهران پرویز شایان گروه انگل شناسی دانشکده دامپزشکی دانشگاه تهران حمید رضا حداد زاده گروه انگل شناسی دانشکده دامپزشکی دانشگاه تهران Journal Article 1970 01 01 This study conducted during 3 years period (2002-2004). Tick sampling was randomly carried out from domestic animals during seasonal activity of ticks from six provinces of Iran. 2170 ticks from 151 cattle , 629 sheep, 336 goats and 32 camels were collected proin 30 prosseces with a mean number of 1.9 tick per animal. 209 Dermacentor ticks collected from six provinces( Kordestan, Ardebil, East Azarbaiejan, Zanjan, Khorasan and Semnan) that included 23% of collected tick population in those provinces. The diversity of Dermacentor is restricted to three species; D.niveus (50%), D.marginatus (27%) and D.raskemensis (23%). The maximum occurance of D.raskemensis , D.niveus, D.marginatus, were occurred in provinces of Semnan, Khorasan and Kordestan, respectively. It can be concluded that livestock had almost different pattern of tick species in any localities thus distribution of D.raskemensis , D.niveus, D. marginatus, has been confirmed this matter. This study conducted during 3 years period (2002-2004). Tick sampling was randomly carried out from domestic animals during seasonal activity of ticks from six provinces of Iran. 2170 ticks from 151 cattle , 629 sheep, 336 goats and 32 camels were collected proin 30 prosseces with a mean number of 1.9 tick per animal. 209 Dermacentor ticks collected from six provinces( Kordestan, Ardebil, East Azarbaiejan, Zanjan, Khorasan and Semnan) that included 23% of collected tick population in those provinces. The diversity of Dermacentor is restricted to three species; D.niveus (50%), D.marginatus (27%) and D.raskemensis (23%). The maximum occurance of D.raskemensis , D.niveus, D. marginatus, were occurred in provinces of Semnan, Khorasan and Kordestan, respectively. It can be concluded that livestock had almost different pattern of tick species in any localities thus distribution of D.raskemensis , D.niveus, D. marginatus, has been confirmed this matter. انتشار جغرافیایی و ایران درماسنتورراسکمنسیس درماسنتور مارژیناتوس درماسنتورنی وئوس https://jvr.ut.ac.ir/article_23937_571cf2f01532717432da4cbd1986b688.pdf
دانشگاه تهران مجله تحقیقات دامپزشکی (Journal of Veterinary Research) 2008-2525 63 2 2008 06 21 EXPERIMENTAL INDUCTION OF NECROTIC ENTERITIS IN BROILER CHICKENS BY CLOSTRIDIUM PERFRINGENS ISOLATES FROM THE OUTBREAKS IN IRAN ایجاد تجربی بیماری آنتریت در جوجه های گوشتی با استفاده از جدایه های PERFRINGENS CLOSTRIDIUM از موارد حاد بیماری در ایران 128 132 23938 FA بهرام شجاعدوست گروه علوم درمانگاهی دانشکده دامپزشکی دانشگاه تهران سید مصطفی پیغمبری گروه علوم درمانگاهی دانشکده دامپزشکی دانشگاه تهران Journal Article 1970 01 01 To establish an stable challenge system for induction of necrotic enteritis (NE) in broiler chickens, different potential factors and predisposing conditions were imposed in 6 separate different experiments using 525 day-old chicks in total. Despite the presence of some predisposing factors and using 4 isolates of Clostridium perfringens ( CP ) from acute and severe NE outbreaks as challenge bacteria, the disease was not successfully reproduced in the first 4 experiments. In the 5th and 6th experiments, the predisposing conditions were changed and each bird was challenged with 3 ml (3×108 CFU/ml) of CP live culture via oral route and also the feed mixed with CP suspension 2 times per day and for 5 consecutive days. Clinical signs and mortalities, and lesions associated with NE were observed in the later two experiments. This study showed that by stressful nutritional and management procedures such as increased stocking density and high levels of wheat and fish meal; induction of relative immunosuppression; using sufficient CP concentration and appropriate ways of its multiplication; and applying challenge bacteria isolated from acute outbreaks, NE may be experimentally induced in broiler chickens. To establish an stable challenge system for induction of necrotic enteritis (NE) in broiler chickens, different potential factors and predisposing conditions were imposed in 6 separate different experiments using 525 day-old chicks in total. Despite the presence of some predisposing factors and using 4 isolates of Clostridium perfringens ( CP ) from acute and severe NE outbreaks as challenge bacteria, the disease was not successfully reproduced in the first 4 experiments. In the 5th and 6th experiments, the predisposing conditions were changed and each bird was challenged with 3 ml (3×108 CFU/ml) of CP live culture via oral route and also the feed mixed with CP suspension 2 times per day and for 5 consecutive days. Clinical signs and mortalities, and lesions associated with NE were observed in the later two experiments. This study showed that by stressful nutritional and management procedures such as increased stocking density and high levels of wheat and fish meal; induction of relative immunosuppression; using sufficient CP concentration and appropriate ways of its multiplication; and applying challenge bacteria isolated from acute outbreaks, NE may be experimentally induced in broiler chickens. آنتریت نکروتیک بیماری تجربی جوجه گوشتی کلستریدیوم پرفرینجنس https://jvr.ut.ac.ir/article_23938_e69a23e09cce75e5738d626e6e6efbb6.pdf
دانشگاه تهران مجله تحقیقات دامپزشکی (Journal of Veterinary Research) 2008-2525 63 2 2008 06 21 THE EFFECT OF EGG DERIVED SPECIFIC ANTIBODY AND PROBIOTIC ON PREVENTION OF SALMONELLA ENTERITIDIS INFECTION IN BROILER CHICKENS تاثیر آنتی بادی استخراج شده از تخم مرغ و پروبیوتیک در پیشگیری ازعفونت سالمونلا انتریتیدس در جوجه های گوشتی 133 139 23939 FA شعبان رحیمی گروه پرورش و تولید طیور دانشکده کشاورزی دانشگاه تربیت مدرس تهران شعبان رحیمی گروه پرورش و تولید طیور دانشکده کشاورزی دانشگاه تربیت مدرس تهران تقی زهرایی صالحی گروه میکروبیولوژی دانشکده دامپزشکی دانشگاه تهران 0000-0002-5665-5757 Journal Article 1970 01 01 To evaluate the effect of probiotic (Primalac) and Salmonella enteritidis-specific IgY on prevention of Salmonella enteritidis infection in broiler chickens, four 33 week-old Single Comb White Leghorn hens were hyperimmunized with Salmonella enteritidis (SE) whole cell antigens obtained by ultrasonication and administrated at a protein concentration of 500 ?g/ml after centrifugation. Primary immunization was performed with 250?g of the antigen prepared in equal volume of Freunds complete adjuvant and saline. Booster injections were done each 14 days for twice,using incomplete Freunds adjuvant. Bleedings were performed 20 days after first injection and eggs were collected. The presence of anti-Salmonella antibody IgY and IgG in egg yolk and serum respectively, was monitored by ELISA, during the immunization period. Body weight, feed intake and feed conversation were determined. Then two hundred forty male “Ross “day-old chicks were randomly assigned to 8 groups and 3 replications of 10 birds were grown for 56 days of experiment. Eight experimental groups identified with, S, P, A, SP, SA, AP, SPA, C. Four birds from four challenged groups(S), were orally inoculated with 0.5 Ml of S. enteritids that contained 1×106 cfu / ml on day 7.The groups that supplemented with antibody (A), received 15 ml of yolk contained antibody(1.5 ml/bird/day), from day 1 to end of the experiment. The probiotic treated groups(P) were received probiotic, 0.1% of feed and 0.5% of feed, during 1-21 and 22-56 days of experimental period respectively. One group as control© did not receive any treatment of probiotic and antibody. To evaluate the effect of probiotic (Primalac) and Salmonella enteritidis-specific IgY on prevention of Salmonella enteritidis infection in broiler chickens, four 33 week-old Single Comb White Leghorn hens were hyperimmunized with Salmonella enteritidis (SE) whole cell antigens obtained by ultrasonication and administrated at a protein concentration of 500 ?g/ml after centrifugation. Primary immunization was performed with 250?g of the antigen prepared in equal volume of Freunds complete adjuvant and saline. Booster injections were done each 14 days for twice, using incomplete Freunds adjuvant. Bleedings were performed 20 days after first injection and eggs were collected. The presence of anti-Salmonella antibody IgY and IgG in egg yolk and serum respectively, was monitored by ELISA, during the immunization period. Body weight, feed intake and feed conversation were determined. Then two hundred forty male “Ross “day-old chicks were randomly assigned to 8 groups and 3 replications of 10 birds were grown for 56 days of experiment. Eight experimental groups identified with, S, P, A, SP, SA, AP, SPA, C. Four birds from four challenged groups(S), were orally inoculated with 0.5 Ml of S. enteritids that contained 1×106 cfu / ml on day 7. The groups that supplemented with antibody (A), received 15 ml of yolk contained antibody(1.5 ml/bird/day), from day 1 to end of the experiment. The probiotic treated groups(P) were received probiotic, 0.1% of feed and 0.5% of feed, during 1-21 and 22-56 days of experimental period respectively. One group as control© did not receive any treatment of probiotic and antibody. الایزا ایمونوگلوبین G ایمونوگلوبین Y پروبیوتیک سالمونلا انتریتیدیس https://jvr.ut.ac.ir/article_23939_70780d0eed698c5169138850f780199e.pdf
دانشگاه تهران مجله تحقیقات دامپزشکی (Journal of Veterinary Research) 2008-2525 63 2 2008 06 21 DETECTION OF TUMOR ANTIGENS IN BOVINE VIRAL LYMPHOSARCOMA BY IMMUNOPRECIPITATION METHOD تشخیص آنتی ژن های توموری در عقده لمفاوی گاو مبتلا به لنفوسارکوم ویروسی با روش رسوب ایمنی 141 146 23940 FA غلامرضا نیکبخت بروجنی گروه میکروبیولوژی دانشکده دامپزشکی دانشگاه تهران سید مهدی امام گروه میکروبیولوژی دانشکده دامپزشکی دانشگاه تهران ندا برجسته گروه میکروبیولوژی دانشکده دامپزشکی دانشگاه تهران Journal Article 1970 01 01 In this study One lymph node from cow with viral lymphosarcoma and one lymph node from healthy cow were analyzed by three different methods for protein extraction, include simple homogenizing in PBS, sonication and phenol extraction. Tumor antigen detection relies on immunoprecipitation followed by SDS-PAGE. In this experiment Streptococcus pyogenes was used for purification of immune complexes. Eletrophoretic patterns were obtained using reduced and non reduced buffers. In PBS homogenization 3 distinct antigens (39, 32 and 30 kDa) were observed. When sonication or phenol extraction were used, 5 (72, 48, 42, 32 and 30 kDa) and 6 (104, 77, 54, 32, 30 and 26, 5 kDa) distinct antigens were observed, respectively. This study showed that the ability of antigen detection mainly depends on protein extraction procedure and antigen-antibody equilibrium achieved by quantification of precipitins. Immunoprecipitation could be used successfully for study of tumor antigens in bovine leukosis. In this study One lymph node from cow with viral lymphosarcoma and one lymph node from healthy cow were analyzed by three different methods for protein extraction, include simple homogenizing in PBS, sonication and phenol extraction. Tumor antigen detection relies on immunoprecipitation followed by SDS-PAGE. In this experiment Streptococcus pyogenes was used for purification of immune complexes. Eletrophoretic patterns were obtained using reduced and non reduced buffers. In PBS homogenization 3 distinct antigens (39, 32 and 30 kDa) were observed. When sonication or phenol extraction were used, 5 (72, 48, 42, 32 and 30 kDa) and 6 (104, 77, 54, 32, 30 and 26, 5 kDa) distinct antigens were observed, respectively. This study showed that the ability of antigen detection mainly depends on protein extraction procedure and antigen-antibody equilibrium achieved by quantification of precipitins. Immunoprecipitation could be used successfully for study of tumor antigens in bovine leukosis. آنتی ژن های توموری رسوب ایمنی لکوزگاوی لمفوسارکوم https://jvr.ut.ac.ir/article_23940_7164b59215bbed7c527dafc1f1e4015d.pdf
دانشگاه تهران مجله تحقیقات دامپزشکی (Journal of Veterinary Research) 2008-2525 63 2 2008 06 21 COMPARATIVE EVALUATION OF LAPAROSCOPIC AND OPEN ELECTIVE OVARIOHYSTERECTOMY IN DOGS ارزیابی مقایسه ای برداشت رحم و تخمدان به روش جراحی لاپاروسکوپیک و باز در سگ 147 151 23941 FA جلال بختیاری گروه علوم درمانگاهی دانشکده دامپزشکی دانشگاه تهران سارا مکرم گروه علوم درمانگاهی دانشکده دامپزشکی دانشگاه تهران علیرضا خلج گروه جراحی دانشکده پزشکی دانشگاه شاهد تهران داوود شریفی گروه علوم درمانگاهی دانشکده دامپزشکی دانشگاه تهران جواد اشرفی هلان گروه پاتوبیولوژی آموزشکده دامپزشکی دانشگاه تبریز 0000-0001-9496-2064 آذین توکلی گروه علوم درمانگاهی دانشکده دامپزشکی دانشگاه تهران Journal Article 1970 01 01 Laparoscopic and open ovariohysterectomy were compared for the following parameters: surgical time, incision length, suture number, saturation of oxyhemoglobin (SpO2), heart rate, complications,CBC findings, Melbourne pain scale (MPS) and gross pathology. MPS pain scores were evaluated using Mann-whitney U Test. Other parameters were evaluated using Studen's t test. There were no significant differences in SPo2, heart rate, surgical complications, MPS pain scores and CBC, where as the blood loss was significantly (P<0.05) lower and the total incision length and suture number was less in laparoscopic group. There were less surgical time and more extensive adhesion formation in the open technique. The laparoscopic ovariohysterectomy is a potentially safe surgical technique in dogs and leads to less adhesion formation that may result in pain of adhesion sites and movment limitation. Laparoscopic and open ovariohysterectomy were compared for the following parameters: surgical time, incision length, suture number, saturation of oxyhemoglobin (SpO2), heart rate, complications, CBC findings, Melbourne pain scale (MPS) and gross pathology. MPS pain scores were evaluated using Mann-whitney U Test. Other parameters were evaluated using Studen's t test. There were no significant differences in SPo2, heart rate, surgical complications, MPS pain scores and CBC, where as the blood loss was significantly (P<0.05) lower and the total incision length and suture number was less in laparoscopic group. There were less surgical time and more extensive adhesion formation in the open technique. The laparoscopic ovariohysterectomy is a potentially safe surgical technique in dogs and leads to less adhesion formation that may result in pain of adhesion sites and movment limitation. ارزیابی درد برداشت رحم و تخمدان سگ لاپاروسکوپی https://jvr.ut.ac.ir/article_23941_a4fa409460351e5ab7bc0b872d69fe54.pdf
دانشگاه تهران مجله تحقیقات دامپزشکی (Journal of Veterinary Research) 2008-2525 63 2 2008 06 21 STUDY ON THE HISTOPATHOLOGICALAND ENZYMATIC RELATIONSHIP IN CHICKENS AFFECTED BYMAREK'S DISEASE بررسی رابطه ضایعات هیستوپاتولوژیک با تغییرات آنزیم های بافتی در ماکیان مبتلا به بیماری مارک 153 158 23942 FA سعید نظیفی گروه علوم درمانگاهی، دانشکده دامپزشکی دانشگاه شیراز عزیزاله خداکرم تفتی گروه پاتوبیولوژی، دانشکده دامپزشکی دانشگاه شیراز مریم انصاری لاری گروه بهداشت مواد غذایی، دانشکده دامپزشکی دانشگاه شیراز مریم کارگر دانش آموخته دانشکده دامپزشکی دانشگاه شیراز Journal Article 1970 01 01 The aim of the present study was to determine the relationship between histopathological lesions and tissue enzymes in chickens affected by Marek's disease.Five apparently healthy chickens(as control group)and 25 chickens affected by Marek's disease were selected. After consideration of history and gross lesions, tissue samples were collected from kidney, liver, heart, ovary, pectoral muscle, spleen, crop, proventriculus and bursa of fabricius for histopathological and tissue enzyme studies. In tissue samples, the activity of aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), creatine kinase (CK), superoxide dismutase (SOD)and glutathione peroxidase (GPX)was measured. Histopathologically, lymphomatous lesions in visceral organs were included in local or diffuse ple omorphic small to medium lymphocytes, lymphoblasts,marek cells and rarely plasma cells. The results indicated that the activities of AST, LDH, CK, SOD and GPX were decreased in the crop of affected chickens. The activities of CK, LDH and AST in the liver tumors and the activities of LDH, AST, ALP, SOD, CK and GPX in pectoral muscles were increased. In proventriculus, the activities of AST, ALP, LDH and SOD were shown to be increased,but the activity of CK was decreased. The activities of AST and SOD in the ovary lesions and the activities of SOD, AST, CK and LDH in the heart lesions showed an increase. Fluctuations in the activity of enzymes in different tissues showed that measurement of these enzymes can not be used as a tumor marker in the diagnosis of Marek's disease. The aim of the present study was to determine the relationship between histopathological lesions and tissue enzymes in chickens affected by Marek's disease.Five apparently healthy chickens(as control group)and 25 chickens affected by Marek's disease were selected. After consideration of history and gross lesions, tissue samples were collected from kidney, liver, heart, ovary, pectoral muscle, spleen, crop, proventriculus and bursa of fabricius for histopathological and tissue enzyme studies. In tissue samples, the activity of aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), creatine kinase (CK), superoxide dismutase (SOD)and glutathione peroxidase (GPX)was measured. Histopathologically, lymphomatous lesions in visceral organs were included in local or diffuse ple omorphic small to medium lymphocytes, lymphoblasts, marek cells and rarely plasma cells. The results indicated that the activities of AST, LDH, CK, SOD and GPX were decreased in the crop of affected chickens. The activities of CK, LDH and AST in the liver tumors and the activities of LDH, AST, ALP, SOD, CK and GPX in pectoral muscles were increased. In proventriculus, the activities of AST, ALP, LDH and SOD were shown to be increased, but the activity of CK was decreased. The activities of AST and SOD in the ovary lesions and the activities of SOD, AST, CK and LDH in the heart lesions showed an increase. Fluctuations in the activity of enzymes in different tissues showed that measurement of these enzymes can not be used as a tumor marker in the diagnosis of Marek's disease. آنزیم بافت بیماری مارک جوجه هیستوپاتولوژی https://jvr.ut.ac.ir/article_23942_d776dc3bfd5cd2e4aa7b65e70fe3bc20.pdf
دانشگاه تهران مجله تحقیقات دامپزشکی (Journal of Veterinary Research) 2008-2525 63 2 2008 06 21 THE EFFECTOFINTRAPERITONEALINJECTION OF HISTIDINE ON DEFECATION RATE IN RABBITS اثر تزریق داخل صفاقی هیستیدین بر میزان دفع مدفوع در خرگوش 159 162 23943 FA غلامرضا وفایی سیاح گروه دامپزشکی دانشگاه آزاد اسلامی واحد ابهر اسماعیل تمدنفرد گروه علوم پایه دانشکده دامپزشکی دانشگاه ارومیه Journal Article 1970 01 01 Histidine at the doses 30 and 60ml /kg had no effect when injected at 7 :OOh, or at 19:00h. Histidine at the doses of 120, 240 and 480 mg / kg decreased defecation at the first 8 and 24 hours post injection, respectively, when injected at 07:00h. The amino acid at the doses of 120, 240 and 480 mg / kg decreased the number of fecal pellets at 2, 8 and 24 h post injection, respectively, when injected at 19:00h. Suppressive effect of histidine in the light period was more intense and lasted longer than that in the dark period. The mean number of pellets for each individual prior to the interventional study was recorded as interval negative control. Histidine at the doses 30 and 60ml /kg had no effect when injected at 7 :OOh, or at 19:00h. Histidine at the doses of 120, 240 and 480 mg / kg decreased defecation at the first 8 and 24 hours post injection, respectively, when injected at 07:00h. The amino acid at the doses of 120, 240 and 480 mg / kg decreased the number of fecal pellets at 2, 8 and 24 h post injection, respectively, when injected at 19:00h. Suppressive effect of histidine in the light period was more intense and lasted longer than that in the dark period. The mean number of pellets for each individual prior to the interventional study was recorded as interval negative control. پلت های مدفوع خرگوش هیستیدین https://jvr.ut.ac.ir/article_23943_38a66a6fa01cd3635f3c2eb80ef2b275.pdf
دانشگاه تهران مجله تحقیقات دامپزشکی (Journal of Veterinary Research) 2008-2525 63 2 2008 06 21 STUDY ON GASTROINTESTINAL HELMINTHES OF DOMESTIC DOGS IN SHIRAZ بررسی آلودگی های کرمی دستگاه گوارش سگ های خانگی شهر شیراز 163 164 23944 FA سعید بکایی گروه بهداشت و کنترل مواد غذایی دانشکده دامپزشکی دانشگاه تهران محمد موذنی گروه پاتوبیولوژی، دانشکده دامپزشکی دانشگاه شیراز مرتضی رودگر دانش آموخته دانشکده دامپزشکی دانشگاه شیراز Journal Article 1970 01 01 Gastrointestinal helminthes in domestic dogs in Shiraz and the effect of age, sex, anthihelminth drugs, use of dog (pet or guard) and food on the infection rate were studied in 105 fecal samples.Samples were collected directly from the rectum and examined by floatation method. Data was analyzed by Chi square test and Fisher's exact test. No significant differences were found in male and female dogs and also different age groups. Higher infection rate was observed in guard dogs in comparison with pet dogs. The infection rate was higher in dogs feeding on viscera or uncooked food. The awareness of owner's about the zoonotic helminthes infection was unsatisfactory.However according to risk of communicable helminthes educational programs for owners are strictly recommended. Gastrointestinal helminthes in domestic dogs in Shiraz and the effect of age, sex, anthihelminth drugs, use of dog (pet or guard) and food on the infection rate were studied in 105 fecal samples. Samples were collected directly from the rectum and examined by floatation method. Data was analyzed by Chi square test and Fisher's exact test. No significant differences were found in male and female dogs and also different age groups. Higher infection rate was observed in guard dogs in comparison with pet dogs. The infection rate was higher in dogs feeding on viscera or uncooked food. The awareness of owner's about the zoonotic helminthes infection was unsatisfactory. However according to risk of communicable helminthes educational programs for owners are strictly recommended. انگل کرمی دستگاه گوارش سگ خانگی شیراز https://jvr.ut.ac.ir/article_23944_5779b0ec15a2179dbe34c8b6f5d51dfe.pdf