University of Tehran PressJournal of Veterinary Research2008-252561220060723--16530FAJournal Article19700101Objective: To determine the effects of adding of different supplemental fats to flushing diet on reproductive parameters in Iranian Zandi fat-tailed ewes.
Design: Randomized complete block.
Animals: Fifty two Six -year-old Zandi ewes.
Procedure: Four experimental diets: without supplemental fat(1), containing 4.5% calcium salts of fatty acids from tallow(2), containing 4.5% calcium salts of fatty acids from soybean oil(3) and containing 2.25% calcium salts of fatty acids from tallow plus 2.25% calcium salts of fatty acids from soybean oil(4) were tested. After laparoscopy, follicles (3 mm diameter) and corpus luteums (CL) on both ovaries were counted. Number of CLs were designated as ovulation rate (OR) index. At lambing number, weight and sex of lambs and lambing date of ewes were recorded.
Statistical analysis: Analysis of variance was done by general linear model procedure of the SAS.
Results: OR in group 3 was higher than the other ones (p<O.OS). Number of follicles in group 2 were lower than the other groups (p<O.O5). Pregnancy rate from first, total of two and three first service periods in group 3 were higher than the other groups (p<O.O5). Pregnancy rate from first and total of two first service periods in groups 2 and 4 were higher than group l(p<O.O5). Lambing rate and lamb crop from each of the three service periods were highest in group 3 and lowest in group 1 (p<O.O5). Twining rate from the first service period in group 3 was higher than, the other groups and in groups 2 and 4 was higher than group l(p<O.O5). Twining rate from total of two first service periods in groups 3 and 4 was higher than group l(p<O.O5). Clinical implications: Fat supplementation especially from rich sources of unsaturated fatty acids to flushing diet had positive effect on the OR and reproduction performance of ewes. J.Fac. Vet. Med. Univ. Tehran. 61,2:101-106,2006.Objective: To determine the effects of adding of different supplemental fats to flushing diet on reproductive parameters in Iranian Zandi fat-tailed ewes.
Design: Randomized complete block.
Animals: Fifty two Six -year-old Zandi ewes.
Procedure: Four experimental diets: without supplemental fat(1), containing 4.5% calcium salts of fatty acids from tallow(2), containing 4.5% calcium salts of fatty acids from soybean oil(3) and containing 2.25% calcium salts of fatty acids from tallow plus 2.25% calcium salts of fatty acids from soybean oil(4) were tested. After laparoscopy, follicles (3 mm diameter) and corpus luteums (CL) on both ovaries were counted. Number of CLs were designated as ovulation rate (OR) index. At lambing number, weight and sex of lambs and lambing date of ewes were recorded.
Statistical analysis: Analysis of variance was done by general linear model procedure of the SAS.
Results: OR in group 3 was higher than the other ones (p<O.OS). Number of follicles in group 2 were lower than the other groups (p<O.O5). Pregnancy rate from first, total of two and three first service periods in group 3 were higher than the other groups (p<O.O5). Pregnancy rate from first and total of two first service periods in groups 2 and 4 were higher than group l(p<O.O5). Lambing rate and lamb crop from each of the three service periods were highest in group 3 and lowest in group 1 (p<O.O5). Twining rate from the first service period in group 3 was higher than, the other groups and in groups 2 and 4 was higher than group l(p<O.O5). Twining rate from total of two first service periods in groups 3 and 4 was higher than group l(p<O.O5). Clinical implications: Fat supplementation especially from rich sources of unsaturated fatty acids to flushing diet had positive effect on the OR and reproduction performance of ewes. J.Fac. Vet. Med. Univ. Tehran. 61,2:101-106,2006.https://jvr.ut.ac.ir/article_16530_156e33003510c779d3311361b5c944e7.pdfUniversity of Tehran PressJournal of Veterinary Research2008-252561220060723--16531FAJournal Article19700101Objective: Study on some enzymes and protein
electrophoretic patterns in order to find an indicator for
adequacy of heat treatment in meat products. Design: Experimental study. Procedure: The activities of some enzymes, including:
lactate dehydrogenase (LDH), aspartate amino .transferase
(AST) and alanine amino transferase (ALT), were assayed in meat and heat treated meat products at different time-
temperatures combinations. Extracts of samples were used
for electrophoresis by SDS-PAGE method.
Statistical analysis: Analysis of variance and Duncan’s
test.
Results: LDH was active and demonstrated a good
stability in samples which were heated up to 65°C for 55
minute. However, its activity started to decline thereafter
so that at 70°C no significant activity was observed. ALT
and AST were more heat stable than LDH and their
activities were still present in heat treated products at 70CC
for 30 minutes and vanished at 75°C. Many protein bands
disappeared in SDS-PAGE pattern of meat products which
heated at 65°C or above.
Conclusion: LDH can be considered as a suitable indicator
for meat products that have been heated at 70°C or above.
J.Fac.Vet.Med. Univ. Tehran. 61,2:107-113,2006.Objective: Study on some enzymes and protein
electrophoretic patterns in order to find an indicator for
adequacy of heat treatment in meat products. Design: Experimental study. Procedure: The activities of some enzymes, including:
lactate dehydrogenase (LDH), aspartate amino .transferase
(AST) and alanine amino transferase (ALT), were assayed in meat and heat treated meat products at different time-
temperatures combinations. Extracts of samples were used
for electrophoresis by SDS-PAGE method.
Statistical analysis: Analysis of variance and Duncan’s
test.
Results: LDH was active and demonstrated a good
stability in samples which were heated up to 65°C for 55
minute. However, its activity started to decline thereafter
so that at 70°C no significant activity was observed. ALT
and AST were more heat stable than LDH and their
activities were still present in heat treated products at 70CC
for 30 minutes and vanished at 75°C. Many protein bands
disappeared in SDS-PAGE pattern of meat products which
heated at 65°C or above.
Conclusion: LDH can be considered as a suitable indicator
for meat products that have been heated at 70°C or above.
J.Fac.Vet.Med. Univ. Tehran. 61,2:107-113,2006.https://jvr.ut.ac.ir/article_16531_a168bf6137a41d5308d50d846539d85d.pdfUniversity of Tehran PressJournal of Veterinary Research2008-252561220060723--16532FAJournal Article19700101Objective: To detect and isolate the Gram-positive aerobic and facultative -anaerobic bacteria in hatching eggs.
Design: Prospective longitudinal study.
Animals: One hundred and twenty eggs from the broiler breeder stock and hatchery and 30 new-hatched chicks.
Procedure: Bacteriological studies were done in five different developing stages: immediately after laying, before and after disinfecting, before moving from setter to hatcher and from new hatched chicks. Furthermore, two different samples were taken from the shell and the yolk of each egg. Eggs were washed with Nutrient-Broth Media, stored in 37CC for 24 hours and finally transferred to blood agar containing potassium telorite. Yolk samples were directly transferred to blood agar containing potassium telorite. This process was repeated similarly for those samples from yolk sac of 18-day-old embryos and newborn chicks.
Statistical analysis: Exact fisher test.
Results: The contamination rate of eggshells in different stages were 96.66%, 100%, 100% and 90% ,respectively. Bacillus cereus, B. subtilis, B. polymixa and B. coagulans were isolated in 44.15%, 64.16%, 7.4% and 2.5% of cases, respectively. Staphylococcus aureus and S. saprophytic us were isolated from one yolk (3.3%) and tow yolk sacs (6.6%). Any bacteria was isolated from yolk sac of 18 - day- old embryos.
Clinical implications: The source of such contamination is mostly related to eggshells. In spite of routine managing programs in breeder stocks (such as selection, cleaning, and disinfection of eggs) it dose not effect to reduce Gram positive bacteria on egg shells and contamination of eggs in setter.Hence, yolk sac infection occurs and causes mortality in chicks. J.Fac.Vet.Med. Univ. Tehran. 61,2:115-118,2006.Objective: To detect and isolate the Gram-positive aerobic and facultative -anaerobic bacteria in hatching eggs.
Design: Prospective longitudinal study.
Animals: One hundred and twenty eggs from the broiler breeder stock and hatchery and 30 new-hatched chicks.
Procedure: Bacteriological studies were done in five different developing stages: immediately after laying, before and after disinfecting, before moving from setter to hatcher and from new hatched chicks. Furthermore, two different samples were taken from the shell and the yolk of each egg. Eggs were washed with Nutrient-Broth Media, stored in 37CC for 24 hours and finally transferred to blood agar containing potassium telorite. Yolk samples were directly transferred to blood agar containing potassium telorite. This process was repeated similarly for those samples from yolk sac of 18-day-old embryos and newborn chicks.
Statistical analysis: Exact fisher test.
Results: The contamination rate of eggshells in different stages were 96.66%, 100%, 100% and 90% ,respectively. Bacillus cereus, B. subtilis, B. polymixa and B. coagulans were isolated in 44.15%, 64.16%, 7.4% and 2.5% of cases, respectively. Staphylococcus aureus and S. saprophytic us were isolated from one yolk (3.3%) and tow yolk sacs (6.6%). Any bacteria was isolated from yolk sac of 18 - day- old embryos.
Clinical implications: The source of such contamination is mostly related to eggshells. In spite of routine managing programs in breeder stocks (such as selection, cleaning, and disinfection of eggs) it dose not effect to reduce Gram positive bacteria on egg shells and contamination of eggs in setter.Hence, yolk sac infection occurs and causes mortality in chicks. J.Fac.Vet.Med. Univ. Tehran. 61,2:115-118,2006.https://jvr.ut.ac.ir/article_16532_51204980cd9d54c8c3610ba285a96bcc.pdfUniversity of Tehran PressJournal of Veterinary Research2008-252561220060723--16533FAJournal Article19700101Objective: A case report of multicentric lymphoma in a dog.
Animal: An eleven-year-old German Shepherd dog.
Procedure: Diagnosis was confirmed on the basis of clinical signs, laboratory and radiographic findings and histopathologic studies.
Results: An 11-year-old female German Shepherd dog was referred to Veterinary Clinic of University of Shiraz with signs of weakness, inappetance and weight loss. Clinical examinations revealed fever and generalized lymphoadenopathy. Hematologic findings showed lymphocytosis.Multicenlric lymphoma was diagnosed using histopathological studies of lymph nodes, bone marrow and internal organs.
J.Fac. Vet.Med. Univ. Tehran. 61,2:119-121,2006.Objective: A case report of multicentric lymphoma in a dog.
Animal: An eleven-year-old German Shepherd dog.
Procedure: Diagnosis was confirmed on the basis of clinical signs, laboratory and radiographic findings and histopathologic studies.
Results: An 11-year-old female German Shepherd dog was referred to Veterinary Clinic of University of Shiraz with signs of weakness, inappetance and weight loss. Clinical examinations revealed fever and generalized lymphoadenopathy. Hematologic findings showed lymphocytosis.Multicenlric lymphoma was diagnosed using histopathological studies of lymph nodes, bone marrow and internal organs.
J.Fac. Vet.Med. Univ. Tehran. 61,2:119-121,2006.https://jvr.ut.ac.ir/article_16533_96b2520fdbe4c4231a7bca7bcb2acd9d.pdfUniversity of Tehran PressJournal of Veterinary Research2008-252561220060723--16534FAJournal Article19700101Objective: Comparison of dimensions and cortical zones
of left and right adrenal glands in Lori-Bakhtiari sheep. Design: Descriptive study. Animals: One hundered pairs of adrenal glands of Lori -
Bakhtiari sheep.
Procedure: One hundered pairs of normal adrenal glands
were collected in two age groups (6 month to 1 year and 1 - 1.5 years old) from Shahrekord abattoir. After collecting
the glands and weighing them, some parameters such as:
length, wide and thickness of them were measured by
caliper device. For histological studies, after tissue
preparation and H&E staining, cortical zones and
medullae of glands were recognized. The size of zones
were determined by micrometry.Values of left and right
adrenal glands and two ages were analyzed. Statistical analysis: t- student test.
Results: There was a significant difference between
dimensions of left and right adrenal glands. The mean
weight and length of left adrenal gland was greater than the
right. In 6 month to 1 year group the mean wide of right
adrenal was greater than left and there was a significant
difference between them. These Findings were in contrast
to 1-1.5 years old group. All of measured parameters in 1-
1.5 years group were larger than 6 month to 1 year group
(P<0.001). Histological studies revealed that, there Was a
significant difference in size of zona fasciculate between ‘left (2766.66±144.33j.tm) and right (3450.00 ± 43.3Opm)
adrenal glands in 1 - 1.5 years group.
Clinical implication: In Lori-Bakhtiari sheep, dimensions
of left adrenal gland was greater than the right one. The size of zona fasciculate in right adrenal gland was greater than the left one in 1-1.5 years group and the difference was
significant (P<0.05). J.Fac. Vet.Med. Univ. Tehran.
61,2:123-127,2006.Objective: Comparison of dimensions and cortical zones
of left and right adrenal glands in Lori-Bakhtiari sheep. Design: Descriptive study. Animals: One hundered pairs of adrenal glands of Lori -
Bakhtiari sheep.
Procedure: One hundered pairs of normal adrenal glands
were collected in two age groups (6 month to 1 year and 1 - 1.5 years old) from Shahrekord abattoir. After collecting
the glands and weighing them, some parameters such as:
length, wide and thickness of them were measured by
caliper device. For histological studies, after tissue
preparation and H&E staining, cortical zones and
medullae of glands were recognized. The size of zones
were determined by micrometry.Values of left and right
adrenal glands and two ages were analyzed. Statistical analysis: t- student test.
Results: There was a significant difference between
dimensions of left and right adrenal glands. The mean
weight and length of left adrenal gland was greater than the
right. In 6 month to 1 year group the mean wide of right
adrenal was greater than left and there was a significant
difference between them. These Findings were in contrast
to 1-1.5 years old group. All of measured parameters in 1-
1.5 years group were larger than 6 month to 1 year group
(P<0.001). Histological studies revealed that, there Was a
significant difference in size of zona fasciculate between ‘left (2766.66±144.33j.tm) and right (3450.00 ± 43.3Opm)
adrenal glands in 1 - 1.5 years group.
Clinical implication: In Lori-Bakhtiari sheep, dimensions
of left adrenal gland was greater than the right one. The size of zona fasciculate in right adrenal gland was greater than the left one in 1-1.5 years group and the difference was
significant (P<0.05). J.Fac. Vet.Med. Univ. Tehran.
61,2:123-127,2006.https://jvr.ut.ac.ir/article_16534_4eb4ea56cffa759caa7a23eda21f1d04.pdfUniversity of Tehran PressJournal of Veterinary Research2008-252561220060723--16535FAJournal Article19700101Objective: This study was carried out to investigste the
seroprevalence of Avian Pneumovirus infection in broiler
breeder farms which were located in Urmia city by using
the enzyme-linked immunosorbent assay (ELISA).
Design: A field survey.
Animal: Brioler breeder chickens. Procedure: In this study, 552 blood samples were taken
from 39 broiler breeder flocks. The broiler breeder flocks
were categorized in three age groups: 0-20, 2 1-35 and 36-
55 weeks old. Serum samples were tested for Avian
Pneumovirus antibodies by using a commercial blocking
ELISA kit.
Statistical analysis: Descriptive statistics.
Results: The results of this study showed,207(37%) of
serum samples were positive and 192 (35%) ones were
negative. Furthermore, 153 (28%) of samples were
suspected. Clinical implications: Based on these findings, it can be
concluded that broiler breeder farms which are located in
Uremia city, are serologically positive in regard to Avian
Pneumovirus infections. Since AVP has economical
importance in poultry industry by inducing respiratory
disease, the prevalence of AVP in prevention and control
programs of respiratory diseases has to be highly
considered. J.Fac. Vet. Med. Univ. Tehran. 61,2:129-
133,2006.Objective: This study was carried out to investigste the
seroprevalence of Avian Pneumovirus infection in broiler
breeder farms which were located in Urmia city by using
the enzyme-linked immunosorbent assay (ELISA).
Design: A field survey.
Animal: Brioler breeder chickens. Procedure: In this study, 552 blood samples were taken
from 39 broiler breeder flocks. The broiler breeder flocks
were categorized in three age groups: 0-20, 2 1-35 and 36-
55 weeks old. Serum samples were tested for Avian
Pneumovirus antibodies by using a commercial blocking
ELISA kit.
Statistical analysis: Descriptive statistics.
Results: The results of this study showed,207(37%) of
serum samples were positive and 192 (35%) ones were
negative. Furthermore, 153 (28%) of samples were
suspected. Clinical implications: Based on these findings, it can be
concluded that broiler breeder farms which are located in
Uremia city, are serologically positive in regard to Avian
Pneumovirus infections. Since AVP has economical
importance in poultry industry by inducing respiratory
disease, the prevalence of AVP in prevention and control
programs of respiratory diseases has to be highly
considered. J.Fac. Vet. Med. Univ. Tehran. 61,2:129-
133,2006.https://jvr.ut.ac.ir/article_16535_f8e26523437a086150954e2b65bd0e55.pdfUniversity of Tehran PressJournal of Veterinary Research2008-252561220060723--16536FAJournal Article19700101Objective: To study the effect of zataria multi flora Boiss essential oil, acetic acid, temperature and storage time on
probable growth of salmonella typhimurium in brain heart
infusion broth.
Design: Multiple factorial analysis of bacterial growth.
Organism: Salmonella ryphimurium.
Procedure: Log probability percentage (Log P%) for
growth of salmonella typhimurium was investigated in Brain Heart Infusion (BHI)broth in response to different
concentration of Zataria multifiora Boiss. essential oil (0.0,
0.03 and 0.06%) and acetic acid (pH,7.4 and 6) during 43
days storage at three temperature (35, 25 and 15°C). Results: The Log P% of S. typhimurium was affected
significantly (Pcz0.05)by different concentrations of
essential oil, pH levels, storage temperature and their
interaction. The Log P% of S. typhimurium in BHI broth
(pH, 7.4) with 0% essential oil at 35, 25 and 15°C were
1.07, 1.07 and 0.41, respectively. This log P% in response
to 0.03 and 0.06% essential oils were -2.93, -3.24 and -
4.23, and -4.23, -4.23 and -4.23, respectively. The Log P%
of S. typhimurium in BHI broth (pH, 6) with 0% essential
oil at 35, 25 and 15°C were 2, 0.76 and 1.41,
respectively. This Logp% in response to 0.03 and 0.06%
essential oils were -1.93, -2.59 and -4.23, and -4.93, -4.93 and -4.93,respectively. At recent conditions, growth has
been completely suppressed.
Food safety implication: Based on the results of this
study, Zataria multifiora Boiss. essential oil can be used as
a growth inhibitor for salmonella in food products.
J.Fac. Vet. Med. Univ. Tehran. 61,2:135-141,2006.Objective: To study the effect of zataria multi flora Boiss essential oil, acetic acid, temperature and storage time on
probable growth of salmonella typhimurium in brain heart
infusion broth.
Design: Multiple factorial analysis of bacterial growth.
Organism: Salmonella ryphimurium.
Procedure: Log probability percentage (Log P%) for
growth of salmonella typhimurium was investigated in Brain Heart Infusion (BHI)broth in response to different
concentration of Zataria multifiora Boiss. essential oil (0.0,
0.03 and 0.06%) and acetic acid (pH,7.4 and 6) during 43
days storage at three temperature (35, 25 and 15°C). Results: The Log P% of S. typhimurium was affected
significantly (Pcz0.05)by different concentrations of
essential oil, pH levels, storage temperature and their
interaction. The Log P% of S. typhimurium in BHI broth
(pH, 7.4) with 0% essential oil at 35, 25 and 15°C were
1.07, 1.07 and 0.41, respectively. This log P% in response
to 0.03 and 0.06% essential oils were -2.93, -3.24 and -
4.23, and -4.23, -4.23 and -4.23, respectively. The Log P%
of S. typhimurium in BHI broth (pH, 6) with 0% essential
oil at 35, 25 and 15°C were 2, 0.76 and 1.41,
respectively. This Logp% in response to 0.03 and 0.06%
essential oils were -1.93, -2.59 and -4.23, and -4.93, -4.93 and -4.93,respectively. At recent conditions, growth has
been completely suppressed.
Food safety implication: Based on the results of this
study, Zataria multifiora Boiss. essential oil can be used as
a growth inhibitor for salmonella in food products.
J.Fac. Vet. Med. Univ. Tehran. 61,2:135-141,2006.https://jvr.ut.ac.ir/article_16536_449f0cbeec0f7add11bdc10467c7116d.pdfUniversity of Tehran PressJournal of Veterinary Research2008-252561220060723--16537FAJournal Article19700101https://jvr.ut.ac.ir/article_16537_d764251b83b47ca87007e48b1415c2ae.pdfUniversity of Tehran PressJournal of Veterinary Research2008-252561220060723--16538FAJournal Article19700101Objective: To compare Pasteur Ella Spp. isolates of the
nasopharyngeal cultures in healthy and unhealthy Holstein
(dairy calf pneumonia) calves.
Design: Case control study.
Animals: Sixty Holstein calves from two weeks up to six- month-old in dairy farms of Mashhad Suburb were
selected.
Procedure: In the affected calves, the clinical findings
were recorded and nasopharyngeal swabs were also taken.
Apparently healthy calves were chosen from the same
farm as control.
Statistical analysis: t-test, Mann-Whitney and Chi-square. Results: Clinical findings including respiratory rate, heart rate, rectal temperature, and illness index scores were
significantly different between healthy and unhealthy
calves (P<O.05). Differences between the isolation rates of
various pathogens from healthy and unhealthy calves were evaluated by contingency table Chi-square analysis.
Pasteurella multicida was isolated more frequently from
nasopharynx in affected group than in control one
(P<O.05). Mannheimia hemolytica was isolated more
frequently in affected ones than controls. However, its
difference was not significant (P>O.05).
Conclusion: Results indicate that Pasteurella multicida
was isolated more frequently from nasopharynx in affected
calves than in control group. However isolation of
Mannheimia hemolytica was not strongly associated with
respiratory disease. J.Fac. Vet. Med. Univ. Tehran.
61,2:147-153,2006.Objective: To compare Pasteur Ella Spp. isolates of the
nasopharyngeal cultures in healthy and unhealthy Holstein
(dairy calf pneumonia) calves.
Design: Case control study.
Animals: Sixty Holstein calves from two weeks up to six- month-old in dairy farms of Mashhad Suburb were
selected.
Procedure: In the affected calves, the clinical findings
were recorded and nasopharyngeal swabs were also taken.
Apparently healthy calves were chosen from the same
farm as control.
Statistical analysis: t-test, Mann-Whitney and Chi-square. Results: Clinical findings including respiratory rate, heart rate, rectal temperature, and illness index scores were
significantly different between healthy and unhealthy
calves (P<O.05). Differences between the isolation rates of
various pathogens from healthy and unhealthy calves were evaluated by contingency table Chi-square analysis.
Pasteurella multicida was isolated more frequently from
nasopharynx in affected group than in control one
(P<O.05). Mannheimia hemolytica was isolated more
frequently in affected ones than controls. However, its
difference was not significant (P>O.05).
Conclusion: Results indicate that Pasteurella multicida
was isolated more frequently from nasopharynx in affected
calves than in control group. However isolation of
Mannheimia hemolytica was not strongly associated with
respiratory disease. J.Fac. Vet. Med. Univ. Tehran.
61,2:147-153,2006.https://jvr.ut.ac.ir/article_16538_70a35178a0428c18271ba90b7ff1639a.pdfUniversity of Tehran PressJournal of Veterinary Research2008-252561220060723--16539FAJournal Article19700101Objective: Evaluation of serum vitamin E status and its Correlation with erythrocyte osmotic fragility in sheep.
Design: Observational study.
Animals: sheep.
Procedure: Blood samples were collected from 223 clinically healthy sheep from different ages of Gezel and Makoei breeds. The animals were assigned into 3 age groups: less than lyear, 1 to 2 years and >2 years old. Serum vitamin E concentrations were determined using HPLC method . Osmotic fragility test (OFT) was carried out by preparing hypotonic saline solution. Mean corpuscular fragility (MCF) was assessed by OFT.
Statistical Analysis: Data was analyzed by SPSS, using one-way analysis of variance (Duncan’s test). Regression correlation analysis was used to estimate an appropriate equation.
Results: Mean and standard deviation of serum vitamin E in sheep less than 1 year, between 1-2 years and >2 years old were 20.78±1.46, 23.31±0.82 and 23.31±0.82 µg/ml and for MCF were 0.52±0.018,0.47±0.018 and 0.49±0.014 g/dl, respectively. Comparison of the means among three groups showed significant differences (P<0.001) for both vitamin E and MCF values. Regression correlation analysis showed that rising the age of the animals increased vitamin E Levels (r=0.64, P<0.001) and decreased MCF values (r=-0.70, P<0.001). There was a negative correlation between vitamin E and MCF values (r=-0.91, P<0.001). According to this finding, estimated regression equation for vitamin could be as Y = -48.24X + 46.20 (r2 = 0.83, P<0.001); Y = Vit.E (µg/ml), X = MCF (g/dl)
Clinical implications: According to the results of this study, it can be concluded that OFT (MCF) is a cheap and easy method for evaluation of vitamin E status in sheep. J.Fac. Vet. Med. Univ. Tehran. 61,2:155-159,2006.Objective: Evaluation of serum vitamin E status and its Correlation with erythrocyte osmotic fragility in sheep.
Design: Observational study.
Animals: sheep.
Procedure: Blood samples were collected from 223 clinically healthy sheep from different ages of Gezel and Makoei breeds. The animals were assigned into 3 age groups: less than lyear, 1 to 2 years and >2 years old. Serum vitamin E concentrations were determined using HPLC method . Osmotic fragility test (OFT) was carried out by preparing hypotonic saline solution. Mean corpuscular fragility (MCF) was assessed by OFT.
Statistical Analysis: Data was analyzed by SPSS, using one-way analysis of variance (Duncan’s test). Regression correlation analysis was used to estimate an appropriate equation.
Results: Mean and standard deviation of serum vitamin E in sheep less than 1 year, between 1-2 years and >2 years old were 20.78±1.46, 23.31±0.82 and 23.31±0.82 µg/ml and for MCF were 0.52±0.018,0.47±0.018 and 0.49±0.014 g/dl, respectively. Comparison of the means among three groups showed significant differences (P<0.001) for both vitamin E and MCF values. Regression correlation analysis showed that rising the age of the animals increased vitamin E Levels (r=0.64, P<0.001) and decreased MCF values (r=-0.70, P<0.001). There was a negative correlation between vitamin E and MCF values (r=-0.91, P<0.001). According to this finding, estimated regression equation for vitamin could be as Y = -48.24X + 46.20 (r2 = 0.83, P<0.001); Y = Vit.E (µg/ml), X = MCF (g/dl)
Clinical implications: According to the results of this study, it can be concluded that OFT (MCF) is a cheap and easy method for evaluation of vitamin E status in sheep. J.Fac. Vet. Med. Univ. Tehran. 61,2:155-159,2006.https://jvr.ut.ac.ir/article_16539_fa34f72f8ec2c39fabd76a671aa35768.pdfUniversity of Tehran PressJournal of Veterinary Research2008-252561220060723--16540FAJournal Article19700101Objective: Study on Giardia spp, among the pet dogs of Isfahan.
Design: Cross-sectional study.
Animals: One hundered and twenty dogs.
Procedure: Faecal specimens of 120 pet dogs which were referrd to the small animal clinic of Isfahan were tested for Giardia spp. The diagnosis of Giardia spp was based on indentification of cycts or trophozoietes in faece. Direct and formalin - ether methods as well as trichorme staining were apllied for identification of Giardia spp.
Statistical analysis: Fisher exact test.
Results: The results indicated that 4 dogs (3.33%) were infected with Giardia spp.
Clinic implication: Because of probable infection of human by this species, our findings recommend killing of stray dogs and treatment of infected ones. J.Fac. Vet. Med. Univ. Tehran. 61,2:161-163,2006.Objective: Study on Giardia spp, among the pet dogs of Isfahan.
Design: Cross-sectional study.
Animals: One hundered and twenty dogs.
Procedure: Faecal specimens of 120 pet dogs which were referrd to the small animal clinic of Isfahan were tested for Giardia spp. The diagnosis of Giardia spp was based on indentification of cycts or trophozoietes in faece. Direct and formalin - ether methods as well as trichorme staining were apllied for identification of Giardia spp.
Statistical analysis: Fisher exact test.
Results: The results indicated that 4 dogs (3.33%) were infected with Giardia spp.
Clinic implication: Because of probable infection of human by this species, our findings recommend killing of stray dogs and treatment of infected ones. J.Fac. Vet. Med. Univ. Tehran. 61,2:161-163,2006.https://jvr.ut.ac.ir/article_16540_674d2c7948a73a437bb6c028d6b97d9d.pdfUniversity of Tehran PressJournal of Veterinary Research2008-252561220060723--16541FAJournal Article19700101Objective: To determine the fauna and prevalence of
parasitic infections of camel (Camelus dromedarius) in
Kerman slaughterhouse.
Design: Sample Survey.
Animal: Sixty slaughtered camel in Kerman slaughterhouse.
Procedure: Examination of different organs (including
alimentary canal, abdominal cavity, liver, lung, kidneys,
heart) and blood smear for parasitic infections were done.
The parasites in the washed contents of alimentary canal,
lung and sliced organ were cleared in lactophenol or
stained with carmine acid collected, counted and indentified under the microscope. Blood smears were stained with Gimsa-stain.
Results: Alimentary tract, liver, lung, nasal cavity and
blood were infected tissues. Eight species of parasites were
detected in abomasum (Homoncus contortus;6.67%),
small intestine (Moniezia expansa,5%; M. benedeni,
6.67%; Stilesia globipunctata, 8.3%), Liver (hydatid cyst,
3.3%), lung (hydatid cyst, 28%; Dictyocaulus fl/aria,
10%), nasal cavity (Cephalopina titillator larvae, 63.3%),
blood: (Trypanosoma evansi, 1.6%).
Conclusion: For the first time we reported these parasites
in camel from Kerman. According to average, minimum
and maximum number of parasites recovered from infected animal no pathogenicity can be noticed for parasite burden
of examined animals. However one camel with 43 larvae of
Cephalopina titilattor in nasal cavity showed clinical
signs, such as sneeze and snort. J.Fac.Vet.Med. Univ.
Tehran. 61,2:165-168,2006.Objective: To determine the fauna and prevalence of
parasitic infections of camel (Camelus dromedarius) in
Kerman slaughterhouse.
Design: Sample Survey.
Animal: Sixty slaughtered camel in Kerman slaughterhouse.
Procedure: Examination of different organs (including
alimentary canal, abdominal cavity, liver, lung, kidneys,
heart) and blood smear for parasitic infections were done.
The parasites in the washed contents of alimentary canal,
lung and sliced organ were cleared in lactophenol or
stained with carmine acid collected, counted and indentified under the microscope. Blood smears were stained with Gimsa-stain.
Results: Alimentary tract, liver, lung, nasal cavity and
blood were infected tissues. Eight species of parasites were
detected in abomasum (Homoncus contortus;6.67%),
small intestine (Moniezia expansa,5%; M. benedeni,
6.67%; Stilesia globipunctata, 8.3%), Liver (hydatid cyst,
3.3%), lung (hydatid cyst, 28%; Dictyocaulus fl/aria,
10%), nasal cavity (Cephalopina titillator larvae, 63.3%),
blood: (Trypanosoma evansi, 1.6%).
Conclusion: For the first time we reported these parasites
in camel from Kerman. According to average, minimum
and maximum number of parasites recovered from infected animal no pathogenicity can be noticed for parasite burden
of examined animals. However one camel with 43 larvae of
Cephalopina titilattor in nasal cavity showed clinical
signs, such as sneeze and snort. J.Fac.Vet.Med. Univ.
Tehran. 61,2:165-168,2006.https://jvr.ut.ac.ir/article_16541_6ccabf88d5afeb53ff2b65aa4771e55e.pdfUniversity of Tehran PressJournal of Veterinary Research2008-252561220060723--16542FAJournal Article19700101https://jvr.ut.ac.ir/article_16542_c5b6af825d84dcb46fe20b208749cd3e.pdfUniversity of Tehran PressJournal of Veterinary Research2008-252561220060723--10119916543FAJournal Article20130928Objectives: Survey on effects of serotonin agonist on the <br />plasma concentrations of growth hormone (GH) and <br />thyroid hormones (T3 and T4TH) <br />Design: Repeated measures (GLM) were used. <br />Animals: Twenty Kurdish lambs 3-4 months old. Procedure: Animals were randomly divided into four <br />groups. The first group (control) received 2 ml normal <br />saline. The second, third and fourth groups received 120, <br />240 and 480 mg/kg body weight L-tryptophan through <br />jugular vein, respectively. Blood samples were collected in <br />three periods including before (days 1, 4, 5 and 6), during <br />(days 7, 8, 9, 10, 11, 12, 13, 14, 15, 18, 22, 26, 30’ and 34) <br />and after (days 36, 37 and 38) injections. Plasma concentrations of these hormones in unextracted samples <br />was analyzed by radioimmunoassay kit (Tabeshyarnoor <br />kits company, Tehran, Iran). <br />Results: Injection of serotonin agonist increased mean <br />plasma concentration (MPC) of GH (P<0.01). There are no significant differences in MPC of GH within the treated groups. Effects of blood sampling times, period (before, <br />during, after injection) and their interaction with treatment <br />on MPC of GH were significant (P<0.01). MPC of TH (T3 <br />and T4) in all of the treated groups was significantly <br />increased compare with control one (P<0.01). There are no <br />significant differences in MPC of TH within the treated <br />groups. Effects of blood sampling times and period <br />(before, during and after injection) and also their <br />interactive effects with treatment were significant. <br />Conclusion: According to statistical analysis of data we can suggest that serotonin agonist stimulates GH and TH <br />secretion simultaneously. J. Fac. Vet.Med. Univ. Tehran. <br />61,2:175-179,2006.Objectives: Survey on effects of serotonin agonist on the <br />plasma concentrations of growth hormone (GH) and <br />thyroid hormones (T3 and T4TH) <br />Design: Repeated measures (GLM) were used. <br />Animals: Twenty Kurdish lambs 3-4 months old. Procedure: Animals were randomly divided into four <br />groups. The first group (control) received 2 ml normal <br />saline. The second, third and fourth groups received 120, <br />240 and 480 mg/kg body weight L-tryptophan through <br />jugular vein, respectively. Blood samples were collected in <br />three periods including before (days 1, 4, 5 and 6), during <br />(days 7, 8, 9, 10, 11, 12, 13, 14, 15, 18, 22, 26, 30’ and 34) <br />and after (days 36, 37 and 38) injections. Plasma concentrations of these hormones in unextracted samples <br />was analyzed by radioimmunoassay kit (Tabeshyarnoor <br />kits company, Tehran, Iran). <br />Results: Injection of serotonin agonist increased mean <br />plasma concentration (MPC) of GH (P<0.01). There are no significant differences in MPC of GH within the treated groups. Effects of blood sampling times, period (before, <br />during, after injection) and their interaction with treatment <br />on MPC of GH were significant (P<0.01). MPC of TH (T3 <br />and T4) in all of the treated groups was significantly <br />increased compare with control one (P<0.01). There are no <br />significant differences in MPC of TH within the treated <br />groups. Effects of blood sampling times and period <br />(before, during and after injection) and also their <br />interactive effects with treatment were significant. <br />Conclusion: According to statistical analysis of data we can suggest that serotonin agonist stimulates GH and TH <br />secretion simultaneously. J. Fac. Vet.Med. Univ. Tehran. <br />61,2:175-179,2006.https://jvr.ut.ac.ir/article_16543_c76640260c8b2423b087a1aba135660d.pdfUniversity of Tehran PressJournal of Veterinary Research2008-252561220060723--16544FAJournal Article19700101Objective: Evaluation of using melatonin implant in rams in
non-breeding season on improvement of reproductive
performance in the ewes.
Design: Clinical trial. Animals: one hundered and seventy seven Atabi ewes and
8 Atabi rams.
Procedure: This study carried out in the following stages:
Stage 1: 177 ewes were complete randomly divided in two
groups. Stage 2: Four rams treated with 12 melatonin
implant (each rams received three implant contain 54 mg
melatonin) and another four rams had not any
treatment. Stage 3: After 35 days, four rams which received
melatonin (treatment group) introduced into the herd of 91
ewes .Four rams that had not any treatment (control group)
introduced into herd of 86 ewes. Then, 60 days later rams
were separated from herds and one month later all ewes
examined for pregnancy through ultrasonography.
Statistical Analysis: Results were analyzed by chi- square test.
Results: Fertility, prolificacy and fecundity values in the
treatment group (ewes wich mated with melatonin treated
rams) and control group (ewes which mated with rams
without melatonin treatment) were 87.1%, 103.8%, 9 [.2%
and 88.4%, 102.6%, 90.7%, respectively. Moreover, there
were not significant differences between them.
Conclusion: we concluded that using melatonin implant in
the Atabi rams had not good results on reproductive index
in the ewes. J.Fac.Vet.Med. Univ. Tehran. 61,2:181-
185,2006.Objective: Evaluation of using melatonin implant in rams in
non-breeding season on improvement of reproductive
performance in the ewes.
Design: Clinical trial. Animals: one hundered and seventy seven Atabi ewes and
8 Atabi rams.
Procedure: This study carried out in the following stages:
Stage 1: 177 ewes were complete randomly divided in two
groups. Stage 2: Four rams treated with 12 melatonin
implant (each rams received three implant contain 54 mg
melatonin) and another four rams had not any
treatment. Stage 3: After 35 days, four rams which received
melatonin (treatment group) introduced into the herd of 91
ewes .Four rams that had not any treatment (control group)
introduced into herd of 86 ewes. Then, 60 days later rams
were separated from herds and one month later all ewes
examined for pregnancy through ultrasonography.
Statistical Analysis: Results were analyzed by chi- square test.
Results: Fertility, prolificacy and fecundity values in the
treatment group (ewes wich mated with melatonin treated
rams) and control group (ewes which mated with rams
without melatonin treatment) were 87.1%, 103.8%, 9 [.2%
and 88.4%, 102.6%, 90.7%, respectively. Moreover, there
were not significant differences between them.
Conclusion: we concluded that using melatonin implant in
the Atabi rams had not good results on reproductive index
in the ewes. J.Fac.Vet.Med. Univ. Tehran. 61,2:181-
185,2006.https://jvr.ut.ac.ir/article_16544_10a3c26d0990dde225c62d0826d97e22.pdfUniversity of Tehran PressJournal of Veterinary Research2008-252561220060723--16545FAJournal Article19700101Objective: Study of histological changes in gonads of fish
received a variety treatment of 1 7-a methyl testosterone.
Animals: Rainbow trout ( Oncorhynchus mykiss).
Design: Descriptive study.
Methods: Application of 17-a methyl testosterone (MT)
using immersion and/or oral administration methods in two
seasons and in 5 and 11 treatments along with a control
group with no hormonal treatment for each season,
respectively. In immersion method eyed eggs and larvae
were immersed in 250 mg/l MT bath for 2 hours twice 4
and 8 days apart. In oral administration method newly
feeding larvae were fed with food containing 0.5, 1, 2, 3
and 30 ppm MT for 60, 70, 90 and 120 days after
commencing active feeding. Histological examination was
carried out on gonads of 20 fish from each treatment as
well as control group at the age of 11 and 24 months for two seasonal groups of fish, respectively.
Results: Masculinization and sterilization were
successfully carried out on rainbow trout by using a variety
treatment of MT. A range of histological changes were
observed in gonads of different treatment groups. This
included histologically normal males, normal females,
intersex, totally atrophic gonads and sterile fish. [n some
testes a number of follicles were seen among the mass of
spermatozoa. In intersex gonads both male and female cell
types were observed alongside each other. Sterile gonads
could be categorized as: sterile males, sterile females as
well as completely sterile containing residual male cells,
residual female cells and no cells at all, respectively. In a
number of specimens spermatozoon cysts were typically
observed.
Conclusion: Application of 17-a methyl testosterone to
rainbow trout at early stages of development could
dramatically change the histological profile of gonads
ranging from functional testes, to interrex, to sterility.
J.Fac.Vet.Med. Univ. Tehran. 61,2:187-193,2006Objective: Study of histological changes in gonads of fish
received a variety treatment of 1 7-a methyl testosterone.
Animals: Rainbow trout ( Oncorhynchus mykiss).
Design: Descriptive study.
Methods: Application of 17-a methyl testosterone (MT)
using immersion and/or oral administration methods in two
seasons and in 5 and 11 treatments along with a control
group with no hormonal treatment for each season,
respectively. In immersion method eyed eggs and larvae
were immersed in 250 mg/l MT bath for 2 hours twice 4
and 8 days apart. In oral administration method newly
feeding larvae were fed with food containing 0.5, 1, 2, 3
and 30 ppm MT for 60, 70, 90 and 120 days after
commencing active feeding. Histological examination was
carried out on gonads of 20 fish from each treatment as
well as control group at the age of 11 and 24 months for two seasonal groups of fish, respectively.
Results: Masculinization and sterilization were
successfully carried out on rainbow trout by using a variety
treatment of MT. A range of histological changes were
observed in gonads of different treatment groups. This
included histologically normal males, normal females,
intersex, totally atrophic gonads and sterile fish. [n some
testes a number of follicles were seen among the mass of
spermatozoa. In intersex gonads both male and female cell
types were observed alongside each other. Sterile gonads
could be categorized as: sterile males, sterile females as
well as completely sterile containing residual male cells,
residual female cells and no cells at all, respectively. In a
number of specimens spermatozoon cysts were typically
observed.
Conclusion: Application of 17-a methyl testosterone to
rainbow trout at early stages of development could
dramatically change the histological profile of gonads
ranging from functional testes, to interrex, to sterility.
J.Fac.Vet.Med. Univ. Tehran. 61,2:187-193,2006https://jvr.ut.ac.ir/article_16545_0e9728e65f0a959996fee2e7a79f872b.pdfUniversity of Tehran PressJournal of Veterinary Research2008-252561220060723--16546FAJournal Article19700101Objective: To Detect invA gene in Salmonella serotypes byPCR.
Samples: Sixty Salmonella strains were isolated from animals and human sources.
Procedure: In this research, 60 isolated Salmonella from animals and human were tested by biochemical tests (such as carbohydrate utilization and urease) and then were serogrouped by Salmonella 0 antisera.The DNA of isolated Salmonella were extracted by Holmes and Quigley method. Two primers (St139 and 5t141) and PCR reagents were used for amplication of invA gene. PCR reaction was carried out in Master cycler. The PCR products were loaded into 1.2% agarose gel and electrophoresed for 60 minutes at 120 V.
Results: All isolates showed biochemical properties of Salmonellae. In PCR assay, target gene (invA gene) with 284bp size were observed in all of strains, which is corresponded with size of positive control and DNA marker. So in this survey all strains had invA gene.
Conclution: Acorrding to the results of this study PCR method based on invA gene is useful for rapid identification of Salmonella serotypes. J.Fac. Vet. Med. Univ. Tehran. 61,2:195-199,2006Objective: To Detect invA gene in Salmonella serotypes byPCR.
Samples: Sixty Salmonella strains were isolated from animals and human sources.
Procedure: In this research, 60 isolated Salmonella from animals and human were tested by biochemical tests (such as carbohydrate utilization and urease) and then were serogrouped by Salmonella 0 antisera.The DNA of isolated Salmonella were extracted by Holmes and Quigley method. Two primers (St139 and 5t141) and PCR reagents were used for amplication of invA gene. PCR reaction was carried out in Master cycler. The PCR products were loaded into 1.2% agarose gel and electrophoresed for 60 minutes at 120 V.
Results: All isolates showed biochemical properties of Salmonellae. In PCR assay, target gene (invA gene) with 284bp size were observed in all of strains, which is corresponded with size of positive control and DNA marker. So in this survey all strains had invA gene.
Conclution: Acorrding to the results of this study PCR method based on invA gene is useful for rapid identification of Salmonella serotypes. J.Fac. Vet. Med. Univ. Tehran. 61,2:195-199,2006https://jvr.ut.ac.ir/article_16546_84aed65627a41b02ddd9e14742693fcb.pdf