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Objective: In this research, protein patterns of 30 isolates of Newcastle disease virus (isolated in Iran) along with two vaccinal
strains (B1 and Lasota) were determined.
Design: Descriptive study.
Samples: These viruses were isolated from Jun.2000 to Oct.2001 out of 507 brains of chicken suspected to infection with virulent NDV. Procedure: Following preparation and inoculation of 507 brain of infected chicken in allantoic cavity of 8 days embryonated eggs, 30 isolates of virus that identified by HI test, were isolated. These samples along with 2 vaccinal strains, B1 and Lasota were purified by high-speed centrifugation and then electrophoresis by SDSPAGE method.
Results: The results of SDS-PAGE test of samples, showed that the isolated viruses posses 80,72,64,43,27 and 23 kDa bands in their protein patterns. No difference in virulent and avirulent vaccinal (B1 and Lasota) strains in protein patterns was observed. Only in three cases of virulent strains of NDV, 48-49 kDa bands were observed instead of 64 kDa band. This protein has not been previously identified. J. Fac. Vet. Med. Univ. Tehran. 58, 1: 61-65, 2003.