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Objective: This study was carried out for identification of Eimeria spp isolated from poultry breeder farms in Iran by PCR. Design: Pop-Gene Analysis.
Animals: Poultry breeder farms.
Procedures: A total of 114 litter samples from poultry breeder farms without previous exposure to anticoccidial vaccine were collected randomly from relatively five different climate regions
of Iran. DNA was extracted from oocysts of samples, using phenol-
chloroform and proteinase-K. Four pairs of specific primers, designated from Internal Transcribed Spacer-l(ITSI) regions of ribosomal DNA of E. acervulina, E. brunetti, E. necatrix, and E. tend/a and one pairs of universal primer BSEF-BSER which
amplify ITS 1 of different Eimeria were used in PCR assay. In tests
on purified genomic DNA from all species of Eimeria isolated from infected samples, each of four primer pairs amplified the ITS!
region of their respective target species only. The PCR products
were analyzed by agarose gel electrophoresis
Results: DNA fragments in sizes of 320 pairs (E. acervulina), 311 pairs (E. brunette), 384 pairs (E. necatrix) and 287 pairs (E. tenella)
were detected on agarose gel electrophoresis. Universal primer
pairs also amplified ITS1 of five Eimeria which isolated from
infected samples. Laboratory implications: The results of this study were showed that PCR technique is a conventional method, faster, technically
easier and very cheaper than other methods to identify the Eimeria
spp. Finally, this technique can be recommended to be a routine
work in well equipped veterinary diagnostic labs in IRAN.