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This study was conducted to evaluate the effect of three different doses of FSH (Fdltropin-V, Vetrepharm Co., Canada) on superovulatcry responses in river buffaloes. Eigtheen buffaloes were selected after rectal examination of reproductive tract and randomly alloated into three groups of six. All the buffaloes received two injections of prostaglandin F2 x (25 mg Lutalyse, Upjhon Co.) 12 days apart. Super ovulation in all groups initiated between days 9 to 12 the estrus cycle. Buffaloes in group 1, 2 and 3 super ovulated with total doses of 300 mg, 400 mg and 500 mg NIH of Folltropin respectively at 12 houres interval in 8 equal doses. All super ovulated buffaloes were inseminated 3 times at the beginning of observed estrus. Embryos were collected non- surgically 6 days after the first insemination. Before embryo recovery, the number of corpora lute a (CLs) and unovulated follicles (UFs) were estimated by rectal palpation of the ovaries. The average number of CLs plus UFs was the highest in group 3 (5.83 ± 0.65). However the highest ovulation rate (number of CLs) was observed in group 2 (4.5 ± 0.81). Maximum embryo plus unfertilized ova (UO) was recovered from group 2 (3 ± 1.52) and lowest from group 3 (1 ± 0.71). From the total number of 27 embryos and UOs recovered from the doners, 10 ova were unfertilized, 13 embryos were in morula stage, 1 embryo in early blast cyst stage and 2 embryos in blast cyst stage. On the basis of our results, the best superovulatory responses were observed in group 2 (400 mg). However, the differences were not statistically significant.