نویسندگان
چکیده
کلیدواژهها
عنوان مقاله [English]
In ordre to compare the proteins of Trichophytonverrucosum, five isolates of this fungus, which obtained from human and animals, were chosen. The fungi were cultured on the specicfic media and incubated at 30 and 37 For preparation of protein extracts, the fungi were cultured in sabouraud’s liquid medium and Bradford method was used to estimate the amount of protein, as well. protein analysis was carried out by using SDS-PAGE with 13% polyacrylamide resolving gel and a discontinious buffer system. Gel staining was done by Coomassie Blue G250 and different bands were observed. Protein Patterns were scanned with a Helena densitometer. Fourteen protein bands with different molecular weights (Dalton), including 92250, 81280, 74990, 72440, 70790, 63100, 60000, 56890, 51880, 48420, 44150, 41680, 29150, 12730, were Detected from the supematant of samples. In the pellet of samples, 16 protein bands were illustrated which were the same as above and the molecular weights of the two remainders were 38020 and 34670 Da. There were no differences between proteins of supemataant and pellet of five Trichophyton verrucosum isolates. It is concluded that the different varieties of Trichophyton verrucosum, in respect to their proteins patterns, are similar.
کلیدواژهها [English]
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