THE ROLE OF COELOMIC FLUID IN FERTILIZATION OF RAINBOW TROUT (ONCORHYNCHUS MYKISS) OOCYTES

Iran is one of the most important Rainbow trout (Oncorhynchus mykiss) producing countries in the world and fingering production is the key factor for this industry. The present experiment was conducted to determine the role of the coelomic fluid in artificial fertilization and to study the interactive effect of different brood fish coelomic fluid on fertilization process during 60 min storage at 4°C.The egg quality was evaluated through the rate of eyed and hatched eggs where these indices applied with and without coelomic fluid. In the first two trials, the rat of egg fertilization with and without coelomic fluid did not exhibit significant difference (p>0.05). Eyeing rates were 97.1% and 97.6% for trial I and 99.1% and 97.7 % for trial II, respectively. In next trial the eyeing and hatching rate were 88.7 % and 86.8 % for the eggs fertilised immediately after stripping wheas for those fertilised 60 min. after integrated storage these values were 86.3% and 84.6 % respectively(p>0.05). we concluded that the coelomic fluid has no interative effect on artificial fertilization and the quality of egg fecundity is not influenced by the fertilisation process during 60 min storage at 4°C.