بررسی سمیت حاد و تحتکشنده علفکش آترازین بر شاخصهای خونی ماهی شیربت (Tor grypus)

نویسندگان

1 دانش آموخته محیطزیست دریا دانشگاه علوم و فنون دریایی خرمشهر، خوزستان- ایران

2 گروه محیط زیست، دانشکده منابع طبیعی دریا دانشگاه علوم و فنون دریایی خرمشهر، خوزستان- ایران

3 گروه علوم درمانگاهی، دانشکده دامپزشکی دانشگاه شهید چمران اهواز، اهواز- ایران

چکیده

زمینه مطالعه: علف‌کش آترازین یکی از مهمترین آلاینده‌های اکوسیستم‌های آبی است که متاسفانه به فور در مزراع نیشکر استان خوزستان مورد استفاده قرار گرفته و موجب آلودگی منابع آبی می‌گردد. هدف: محاسبه سمیت حاد آترازین در ماهی شیربت (Tor grypus) و سپس اثر مسمومیت مزمن این سم بر برخی شاخص‌های خونی می‌باشد. روش کار:  LC50 96 ساعته آترازین در ماهی شیربت با وزن g‌12/1 ±10 با استفاده از نرم‌ افزار Probit تعیین شد. سپس180 قطعه ماهی شیربت با وزن g‌15±102 در 4 گروه (5، 10 و 20% LC50 96 و گروه شاهد) در سه تکرار تقسیم گردیدند. 7، 14 و 21 روز پس از شروع آزمایش خون‌گیری انجام شده و شاخص‌های خون‎شناسی شامل: هموگلوبین، هماتوکریت، تعداد گلبول‌های قرمز و سفید خونی و اندیس‌های گلبولی، در مراحل مختلف مقایسه گردید. نتایج: LC50 96 ساعته آترازین 1- mg l  65 محاسبه شد و تقریباً تمام فاکتورهای خونی تحت تأثیر مسمومیت با این سم قرار گرفت (05/0>p). در مورد اندیس‌های گلبولی، تفاوت معنی‎داری بین تیمارها مشاهده نشد (05/0<p). نتیجه‌گیری‌نهایی: در مجموع با توجه به نتایج، آترازین تأثیر عمیقی بر روی فاکتورهای خونی ماهی شیربت‌ دارد، و قرار گرفتن طولانی مدت در معرض غلظت بالای این سم منجر به ایجاد تغییرات فیزیولوژیک در ماهی شیربت می‌گردد.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of acute and sub-lethal toxicity of herbicide, Atrazine, on hematological parameters of Tor grypus

نویسندگان [English]

  • Ali Khabazian Zadeh 1
  • Ali Dadolahi Sohrab 2
  • Mojtaba Alishahi 3
  • Seyed Hosein Khazaei 2
  • Hosein Mohammad Asgari 2
1 Graduated from the Marine Environment Khorramshahr University of Marine Science and Technology, Khozestan- Iran
2 Department of Environmental Sciences, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khozestan- Iran
3 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
چکیده [English]

BACKGROUND: Atrazine is one of the most important and effective pollutants in aquatic ecosystems. The widest sugar cane farms of the Middle East are located in Khouzestan province, Iran. Large amounts of Atrezine are being used in farming on these farms.                  Objectives: The aims of this study were to investigate acute toxicity (LC50 96 h) of atrazine on Barbus grypus and the effects of chronic toxicity with sub-lethal concentration of atrazine on hematological parameters as well. Methods: 180 B grypus were divided into 4 equal groups (5, 10 and 20% of LC50 96 h and control). Blood samples were taken on days 0, 7, 14 and 21 of experiment. Hematological parameters including hemoglobin level, hematocrit, and red blood cells (RBC), white blood cells (WBC), and globular indices were measured and compared among the groups. Results: LC50 96 h was measured 65 mg l-1. Hematological parameters were  significantly affected by chronic toxicity of atrazine. Hemoglobin, hematocrit and RBC were decreased at all sampling periods in fish exposed to different levels of atrazine (p<0.05). There were no significant changes in globular indice values among the groups in sampling periods (p>0.05). For all samples, WBC value was decreased significantly on day 21 (p<0.05). Conclusions: Based on the results, it can be concluded that chronic atrazine toxicity adversely affects hematological parameters of B. grypus.

کلیدواژه‌ها [English]

  • atrazine
  • hematological parameters
  • sub-lethal toxicity
  • Barbus grypus

مراجع

Abdali, S., Yousefi Jourdehi, A., Kazemi, R., Yazdani, M.A. (2011) Effects of Atrazine (Herbicide) on Blood Biochemical Indices of Grass Carp (Ctenopharhyngoden idella). J Persian Gulf. 2: 51-56.
Atamanalp, M., Yanik, T. (2003) Alterations in hematological parameters of rainbow trout Oncorhynchus mykiss exposed to mancozeb. Turk J Vet Anim Sci. 27: 1213-1217.
Ayoola, S. (2008) Histopathological effects of glyphosate on juvenile African catfish (Clarias gariepinus). Am Eur J Agric Environ Sci. 4: 362-367.
Banaee, M., Sureda, A., Mirvaghefi, A.R., Ahmadi, K. (2011) Effects of diazinon on biochemical parameters of blood in rainbow trout (Oncorhynchus mykiss). Pestic Biochem Physiol. 99: 1-6.
Bekeh Ada, F., Ayotunde, E.O., Bayim, B.P.-R. (2012) Some biological and hematological responses of Oreochromis niloticus juveniles exposed to Atrazine herbicide. Int J Bioflux Society (AACL). 5: 369-379.
Cerdeira, A., Santos, N., Ueta, J., Shuhama, I., Pessoa, M., Smith, S., Lanchote, V. (2004) Atrazine in water and biodegradation in a recharge area of Guarany Aquifer in Brazil. Bull Environ Contamin Toxicol. 73: 117-124.
Correll, D.L., Wu, T.L. (1982) Atrazine toxicity to submersed vascular plants in simulated estuarine microcosms. Aquatic Botany. 14: 151-158.
Devos, S., Bosscher, K.D., Staels, B., Bauer, E., Roels, F., Berghe, W., Haegeman, G., Hooghe, R., Hooghe-Peters, E.L. (2003) Inhibition of cytokine production by the herbicide atrazine: Search for nuclear receptor targets. Biochem Pharmacol. 65: 303-308.
Drastichova, J., Svobodova, Z., Luskova, V., Machova, J.( 2004) Effect of cadmium on hematological indices of common carp (Cyprinus carpio L.). Bull Environ Contam Toxicol. 72: 725-732.
Fischer-Scherl, T., Veeser, A., Hoffmann, R.W., Kühnhauser, C., Negele, R.-D., Ewringmann, T. (1991) Morphological effects of acute and chronic atrazine exposure in rainbow trout (Oncorhynchus mykiss). Arch Environ Contam Toxicol. 20: 454-461.
Forouzangohar, M., Haghnia, G.H., Koocheki, A. (2005) Organic amendments to enhance atrazine and metamitron degradation in two contaminated soils with contrasting textures. Soil & Sedim Contam. 14: 345-355.
Ghosh, P.K., Philip, L. (2004) Atrazine degradation in anaerobic environment by a mixed microbial consortium. Water Res. 38: 2277-2284.
Hussein, S., El-Nasser, M., Ahmed, S. (1996) Comparative studies on the effects of herbicide atrazine on freshwater fish Oreochromis niloticus and Chrysichthyes auratus at Assiut, Egypt. Bull Environ Contam Toxicol. 57: 503-510.
Joshi, P., Bose, M., Harish, D. (2002) Haematological changes in the blood of Clarias batrachus exposed to mercuric chloride. J Ecotoxicol & Environ Monit. 12: 119-122.
Köprücü, S.Ş., Köprücü, K., Ural, M.Ş., İspir, Ü., Pala, M. (2006) Acute toxicity of organophosphorous pesticide diazinon and its effects on behavior and some hematological parameters of fingerling European catfish Silurus glanis. Pestic Biochem Physiol. 86: 99-105.
Kori-Siakpere, O., Ubogu, E.O. (2008) Sublethal haematological effects of zinc on the freshwater fish, Heteroclarias sp. (Osteichthyes: Clariidae). Afr J Biotech. 7: 2068-2073.
Moore, A., Waring, C.P. (2001) The effects of a synthetic pyrethroid pesticide on some aspects of reproduction in Atlantic salmon. Aquatic Toxicol. 52: 1-12.
Nieves-Puigdoller, K., Björnsson, B.T., McCormick, S.D. (2007) Effects of hexazinone and atrazine on the physiology and endocrinology of smolt development in Atlantic salmon. Aquatic Toxicol. 84: 27-37.
Prasad, T., Srinivas, T., Rafi, G.M., Reddy, D. (1991) Effect in vivo of atrazine on haematology and O2 consumption in fish, Tilapia mossambica. Biochem Int. 23: 157-161.
Prasad, T., Srinivas, T., Rafi, M., Reddy, D. (1990) Chronic effect of atrazine on hydromineral balance in the crab. Biochem Int. 22: 435-440.
Ramesh, M., Srinivasan, R., Saravanan, M. (2009) Effect of atrazine (Herbicide) on blood parameters of common carp Cyprinus carpio (Actinopterygii: Cypriniformes). Afr J Environ Sci Technol. 3: 453-458.
Rehwoldt, R. (1978) Investigations into the acute toxicity and some chronic effects of selected herbicides on several freshwater fishes. Appl Ecol J. 4: 316-327.
Rusia, V., Sood, S.K. (1992) Routine haematological tests. In: Mukerjee, Kanai, Medical laboratory technology. (1st ed.) Tata McGraw Hill Publishing Company Limited. USA, New York, chapter 1. p. 252-258.
Rymuszka, A., Siwicki, A.K., Sieroslawska, A. (2007) Determination of modulatory potential of atrazine on selected functions of immune cells isolated from rainbow trout (Oncorhynchus mykiss). Cent Eur J Immunol. 32: 97-100.
Solomon, K.R., Baker, D.B., Richards, R.P., Dixon, K.R., Klaine, S.J., La Point, T.W., Kendall, R.J., Weisskopf, C.P., Giddings, J.M., Giesy, J.P. (1996) Ecological risk assessment of atrazine in North American surface waters. Environ Toxicol Chem. 15: 31-76.
Stoker, T.E., Robinette, C.L., Cooper, R.L.  (1999) Maternal exposure to atrazine during lactation suppresses sucklinginduced prolactin release and results in prostatitis in the adult offspring. Toxicol Sci. 52: 68-79.
Tavares, M.D., Martins, M.L., Nascimento, K.S. (1999) Evaluation of haematological parameters in praractus mesopotamicos. Holmberg (osteichthyes, characidae) with Argulus sp. Crustacea, branchiura) infestation and treatment with organophosphat. Rev Bras. 16: 553-555.
Thrall, M.A., Baker, D.C., Lassen, E.D. (2004) Veterinary Hematology and  Clinical Chemistry. (1st ed.) John Wiley publication. UK, London.

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