اثر تنش اکسیداتیو ناشی از سرب بر عملکرد، وضعیت آنتیاکسیدانی و پاسخهای رفتاری جوجه های گوشتی

نویسندگان

1 دانشکده علوم دامی و صنایع غذایی، دانشگاه کشاورزی و منابع طبیعی رامین خوزستان، ملاثانی، خوزستان، ایران

2 بخش علوم دامی، دانشکده کشاورزی دانشگاه شیراز، شیراز، فارس

چکیده

زمینه مطالعه: تا به امروز، نمونه‌های بی‌شماری از اختلالات بیوشیمیایی، فیزیولوژیک و رفتاری ناشی از رویارویی با سرب در حیوانات گزارش شده است. از سوی دیگر، مکانیسمی که طی آن سرب موجب کاهش عملکرد پرندگان می‌گردد، آشکار نشده است. از اینرو، جهت بررسی این فرضیه که سرب با القای تنش اکسیداتیو و تغییر در وضعیت آنتی‌اکسیدانی و رفتار، بر عملکرد پرنده اثر می‌گذارد، این آزمایش طراحی شد. هدف: این آزمایش برای بررسی اثر تنش اکسیداتیو القا شده توسط سرب بر عملکرد، وضعیت آنتی‌اکسیدانی و رفتار جوجه‌های گوشتی انجام شد. روش کار: 80 قطعه جوجه گوشتی به طور تصادفی به دو تیمار آزمایشی با 4 تکرار (هر تکرار شامل 10 قطعه جوجه گوشتی) اختصاص یافت. جیره‌های آزمایشی شامل 1) جیره پایه بدون سرب (شاهد) و 2) جیره پایه حاوی mg‌200 سرب در کیلوگرم جیره. نتایج: نتایج آزمایش حاضر نشان می‌دهد، استفاده از سرب در کل دوره پرورش، افزایش وزن روزانه را نسبت به تیمار شاهد به طور معنی‌داری کاهش داد (01/0>p) و موجب افزایش معنی‌دار ضریب تبدیل خوراک جوجه‌های گوشتی شد (01/0>p). همچنین، پس از مصرف جیره آلوده با محرک تنش‌زا، مقدار مالون‌دی‌آلدهید و نسبت هتروفیل به لنفوسیت به طور معنی‌داری افزایش و فعالیت آنزیم سوپراکسیددسموتاز و گلوتاتیون پراکسیداز به طور معنی‌داری کاهش یافت (01/0≤p). افزون بر این، استفاده از mg‌200 سرب در جیره موجب افزایش معنی‌دار وضعیت نشسته و رفتار تهاجمی و کاهش معنی‌دار رفتار تغذیه پرندگان شد (01/0>p). نتیجه‌گیری نهایی: نتایج این آزمایش نشان می‌دهد تنش اکسیداتیو ناشی از سرب موجب افزایش ضریب تبدیل خوراک و کاهش عملکرد پرنده می‌شود. از اینرو، مکانیسم پایه پیشنهادی برای بروز چنین پدیده‌ای این است که رویارویی با سرب از طریق اختلال در وضعیت آنتی‌اکسیدانی و رفتار تغذیه‌ای پرنده موجب کاهش عملکرد می‌شود.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of Pb-induced oxidative stress on performance, antioxidant status and behavioral responses in broiler chicken

نویسندگان [English]

  • Rohollah Ebrahimi 1
  • Tahereh mohammad abadi 1
  • Mohsen Sari 1
  • Somayeh Salari 1
  • Mohammad Javad Zamiri 2
  • Mohammad Taghi Beygi Nasiri 1
1 Department of Animal Science, Faculty of Agriculture Science, Ramin Agriculture and Natural Resources University of Khuzestan, Khuzestan, Iran
2 Department of Animal Science, Shiraz University, Fars, Iran
چکیده [English]

BACKGROUND: Lead (Pb) induced oxidative stress is known to suppress growth performance in broiler chickens. The current study was carried out in an attempt to describe the specific underlying mechanisms of such phenomenon. OBJECTIVES: The objective of this study was to investigate the effect of Pb-induced oxidative stress on performance, antioxidant status and behavioral responses of broiler chicken. METHODS: Eighty day-old broiler chicks were randomly assigned to 2 dietary treatment groups of 4 pen replicates, namely i) basal diet containing no lead supplement (control) and ii) basal diet containing 200 mg Pb/kg of diet. RESULTS: The results showed that addition of lead decreased body weight gain (p<0.01) and feed conversion ratio (p<0.01). Also, consumption of contaminated diet significantly increased MDA and H/L Ratio and significantly decreased SOD and GPx activity (p<0.01). Moreover, addition of 200 mg/kg diet significantly increased sitting pasture and aggression behavior and decreased feeding behavior (p<0.01).               CONCLUSIONS: Our data conclude that Pb-induced oxidative stress adversely suppressed feed conversion ratio and growth performance. The proposed underlying mechanism for such phenomenon is Pb-induced oxidative stress by impaired antioxidant status and feeding behavior decreased the growth performance.

کلیدواژه‌ها [English]

  • oxidative stress
  • lead
  • antioxidant status
  • Behavior
  • broiler

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