Effect of Lactobacillus rhamnosus (PTCC 1637) on ruminal detoxification of aflatoxin B1

Authors

1 member of scientific staff/ ACECR mashhad

2 Department of Poultry Science, Faculty of Agriculture, Ferdowsi University, Mashhad, Mashhad, Iran

3 Molecular Medicine Research Department, ACECR, Mashhad, Mashhad, Iran

Abstract

BACKGROUND: Aflatoxins are secondary metabolites due to the growth of molds in animal feed. Lactic acid bacteria are microorganisms that can absorb aflatoxins. Objectives: the effect of the yeast Lactobacillus rhamnosus (PTCC 1637) on Aflatoxin B1 detoxification and absorption of toxin in in vitro (the cow rumen) was investigated. METHODS: For this purpose, the bacteria used in various treatments (live-treated, autoclave, heat-treated, treated with acid 100ºC) was prepared and added to the rumen of cattle. Aflatoxin B1 in different doses (0, 5, 10, 20) ppb in the rumen were added and at times one and two hours were incubated at 37°C. The amount of toxin residues was measured by ELISA using Europroxima kits. RESULTS: The results showed that microorganisms have been treated in an autoclave have the largest amount toxin removal (90.5 percent) (p<0.05). Also with increases the incubation time, the amount of toxin absorbed significantly (78%) increased (p<0.05) and with increasing concentrations of toxin in vitro the bacteria’s ability to absorb toxin increases. Conclusions: As a solution to the livestock feed industry bacterial cell wall or its compounds can be helpful in reducing Aflatoxin B1 toxin.

Keywords

References

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Journal of Veterinary Research
Volume 72, Issue 3 - Serial Number 3
September 2017
Pages 305-311

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