BACKGROUND: Metabolic bone disease is a common disease in the pet reptiles. Early diagnosis of this disease has an important role in the treatment and prevention from complications. Since metabolic bone diseases causes bone density to decrease, it is assumed the CT scan can be used as a suitable method to diagnosis early changes in the bone density. OBJECTIVES: The aim of this study was providing a range of cortical and trabecular Hounsfield Numbers of thoracic and lumbar vertebrae in the healthy green iguana by the aid of computed tomography which can be used as a reference. METHODS: Twelve healthy green iguanas were included in this study and based on the body length were divided into three groups. Complete information about maintenance including maintaining environment and nutrition conditions were recorded in questionnaires designed for this study. Lateral and dorsoventral radiographs were obtained using digital radiology to confirm the health of the skeletal system. To achieve CT plans, the animals were restricted by warping method. After obtaining topogram, with the aid of Osteo Protocol, cross sectional images were acquired from random thoracic and lumbar vertebrae. CT images were subsequently evaluated in Syngo MMWP VE40A Medical Software. The results were analyzed using SPSS statistic software. RESULTS: According to statistic results, the mean Hounsfield Number of thoracic and lumbar vertebrae were presented due to the body length of the green iguana. Since the cortical bone density of lumbar vertebrae in all three groups  varied meaningfully and  only variable “body length” was considered influential on cortical mineral bone density of lumbar vertebrae. Cortical bone density of lumbar vertebrae can be used as a reference for detecting the early bone density changes in green iguana.             CONCLUSIONS: Computed tomography can be an excellent alternative for early detection of the MBD in pet green iguanas.