Indirect immunofluorescent antibody technique (IDFAT) as a rapid tool has been evaluated to detect bacterial infections in farmed fish and prawns. Using this method 107 oxidase positive bacterial isolated obtained from different fish and prawn farmings, were studied from which 20 isolates were identified as Vibrio anguillarum and Aeromonas hydrophila using polyclonal antibodies against these bacterial species. The verification of these results was also demonstrated by studding biochemical/phonotypical characteristics of the bacterial isolates resulting in a high accuracy of IDFAT method (> 70%).
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