-

Objective: To study the effect of zataria multi flora Boiss essential oil, acetic acid, temperature and storage time on
probable growth of salmonella typhimurium in brain heart
infusion broth.
Design: Multiple factorial analysis of bacterial growth.
Organism: Salmonella ryphimurium.
Procedure: Log probability percentage (Log P%) for
growth of salmonella typhimurium was investigated in Brain Heart Infusion (BHI)broth in response to different
concentration of Zataria multifiora Boiss. essential oil (0.0,
0.03 and 0.06%) and acetic acid (pH,7.4 and 6) during 43
days storage at three temperature (35, 25 and 15°C). Results: The Log P% of S. typhimurium was affected
significantly (Pcz0.05)by different concentrations of
essential oil, pH levels, storage temperature and their
interaction. The Log P% of S. typhimurium in BHI broth
(pH, 7.4) with 0% essential oil at 35, 25 and 15°C were
1.07, 1.07 and 0.41, respectively. This log P% in response
to 0.03 and 0.06% essential oils were -2.93, -3.24 and -
4.23, and -4.23, -4.23 and -4.23, respectively. The Log P%
of S. typhimurium in BHI broth (pH, 6) with 0% essential
oil at 35, 25 and 15°C were 2, 0.76 and 1.41,
respectively. This Logp% in response to 0.03 and 0.06%
essential oils were -1.93, -2.59 and -4.23, and -4.93, -4.93 and -4.93,respectively. At recent conditions, growth has
been completely suppressed.
Food safety implication: Based on the results of this
study, Zataria multifiora Boiss. essential oil can be used as
a growth inhibitor for salmonella in food products.
J.Fac. Vet. Med. Univ. Tehran. 61,2:135-141,2006.