In the present work, 200 Fasciola hepatica and Fasciola gigantica were collected from sheep livers. After washing and preparing cell suspention from their genital organs, kayotyping study was carried out using air-drying and squashing methods. The results indicate, eight primary spermatocytes in rossette form of 20 diploid chromosomes as well as individual primary oocyte. The twenty chromosomes consist of one pair of large, three pairs of medium and six pairs of small size. No significant differences between spermatogonial and oogonial as well as length and shape of chromosomes of two species of Fasciola were observed.
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