In order to study the development of knee joint in absence of
movement; mouse limb buds were separated from 15 days old
embryos and cultured in Ham's F-IO medium conditioned with
37°C and 5% CO2 for 1 to 6 days. The medium was renewed every
24h during incubation. After 6 days, specimens were fixed by
Buin's fluid, embedded in paraffin wax, serially sectioned and
stained with H&E. The results indicated that, the mesenchymal
cellsof joint interzone were differentiated to fibroblast like cells and
chondrocytes rather than lossing to form joint space. The
intra-articular structures and joint capsules were also failed to
develop in culture condition. In conclusion it can be postulated
that, the absence of movement and in vitro conditions could affect
the joint development and its intra-articular structures.
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