BACKGROUND: Braxy vaccine, a culture of a suitable strain or strains of Clostridium septicum in a fluid medium, its filtrate or derivatives, will be inactivated so that it produces its immunogenic activity without toxicity. OBJECTIVES: Production of C. septicum vaccine (Braxy vaccine) using enriched culture media in fermenter. METHODS: Conventional and enriched culture media were used for growing C. septicum (CN913) vaccinal strain in both 10 liter glass bottle and fermenter, in a triplicate manner. All twelve experiments were inspected to ensure compliance with the requirements of the vaccine international standards for minimum lethal dose (MLD), sterility, freedom of abnormal toxicity, safety and potency tests. RESULTS: Results showed that the culture of C. septicum in fermenter using enriched culture media is the best. Based on international standards, 2.5IU/mL was determined for C. septicum alpha toxin potency test, but in this study 4IU/mL was obtained. CONCLUSIONS: Using enriched culture media in fermenter is suitable for braxy vaccine production.
Pilehchian Langroudi, R., & Jabari, A. R. (2013). Production of Braxy vaccine by fermenter and enriched culture media. Journal of Veterinary Research, 68(3), 297-304. doi: 10.22059/jvr.2013.35446
MLA
Reza Pilehchian Langroudi; Ahmad Reza Jabari. "Production of Braxy vaccine by fermenter and enriched culture media", Journal of Veterinary Research, 68, 3, 2013, 297-304. doi: 10.22059/jvr.2013.35446
HARVARD
Pilehchian Langroudi, R., Jabari, A. R. (2013). 'Production of Braxy vaccine by fermenter and enriched culture media', Journal of Veterinary Research, 68(3), pp. 297-304. doi: 10.22059/jvr.2013.35446
VANCOUVER
Pilehchian Langroudi, R., Jabari, A. R. Production of Braxy vaccine by fermenter and enriched culture media. Journal of Veterinary Research, 2013; 68(3): 297-304. doi: 10.22059/jvr.2013.35446
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