مقایسه شجره شناسی قطعه ژن ‌1S جدایه‌های ویروس برونشیت عفونی پرندگان در حال چرخش با واکسن‌های مصرفی در ایران

نویسندگان

1 بخش تحقیق و تولید واکسن‌های ویروسی طیور، موسسسه تحقیقات واکسن وسرم سازی رازی، کرج –ایران

2 بخش بیماریهای طیور داشکده دامپزشکی تهران- متخصص بیماریهای طیور

3 گروه میکروبیولوژی و ایمنی شناسی، دانشکده دامپزشکی دانشگاه تهران، تهران–ایران

چکیده

زمینه مطالعه:‌ ‌بیماری برونشیت عفونی پرندگان بسیار مسری، که می‌تواند موجب ضعف در وزن گیری و کاهش کارایی غذا در پرندگان آلوده شود. وجود سروتیپ™ژنوتیپ‌های متعدد گزارش شده، کنترل بیماری را با واکسن مشکل‌تر نموده است. به هر حال تنها معدودی اسیدهای آمینه پروتئین ‌1S مسئـول حفـاظـت در سـویـه‌هـای واکسـن و فیلدی می‌باشند. هدف: ‌مقایسه جدایه‌های ویروس برونشیت در گله‌های طیور صنعتی ایران با واکسن‌های رایج مورد مصرف می‌باشد. روش کار:‌ ‌قطعه‌ای از ژن پروتئین ‌1S که ناحیه پر تغییر و پایدار را پوشش می‌دهد، در جدایه‌های ویروس برونشیت پرندگان ایران با استفاده از روش ‌R‌‌T‌-‌PCR تکثیر، تعیین توالی و مورد مقایسه قرار گرفتند. نتایج:‌ ‌تجزیه و تحلیل فیلوژنی توالی اسیدهای آمینه نشان داد که هشت جدایه از نه جدایه مورد مطالعه با واکسن 52‌H و120H بیش از 8/21% اختلاف و شش جدایه نیز حداقل 7/22%  با سویه واکسن سویه 91/4 و هر نه جدایه با سروتیپ ‌D‌274 حداقل 20% اختلاف داشتند. نتیجه گیری‌نهایی: نتایج بدست آمده برای بررسی مستمر استراتژی‌های کنترل بیماری و نظارت بر سویه‌های در گردش ضروری می‌باشد، بنابراین اهمیت اصلاح برنامه واکسیناسیون را در ایران تأکید می‌نماید.

کلیدواژه‌ها

عنوان مقاله [English]

Phylogenetic analysis comparing partial S1 gene of avian infectious bronchitis virus to commercial vaccine strains in Iran

نویسندگان [English]

  • Masood Hashemzadeh 1
  • Shahin Masoudi 1
  • vahid karimi 2
  • Abdol Hamid Shoshtari 1
  • Arash Ghalyanchi-Langeroudi 3
  • Mohsen Mahmoodzadeh 1
1 Department of Research and Production of Poultry Viral Vaccine, Razi Vaccine and Serum Research Institute, Karaj-Iran
2 Departments of Poulty Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran-Iran
3 Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran-Iran
چکیده [English]

BACKGROUND: Infectious bronchitis is a highly contagious disease which may cause poor weight gain and low feed efficiency in infected chickens. There are a large number of reported serotypes/genotypes, which makes the control of the disease more difficult through vaccination. However, there are only a few amino acid differences in the S1 protein of vaccine and wild type strains which are responsible for protection. OBJECTIVES: The purpose of this study is to compare IBV variants isolated from commercial chicken flocks in Iran with currently used vaccine strains. METHODES: The partial S1 gene of the spike protein, covering a hypervariable and constant regions, was amplified and sequenced using conventional RT-PCR. RESULTS: Phylogenetic analysis of amino acid sequences revealed that eight of total nine isolates were divergence at least 21.8% from vaccinal Massachusetts serotypes, and six of nine isolates were divergence at least 22.7% from 4/91, and none of the nine isolates were similar to Dutch-type, D274,vaccine serotypes. CONCLUSIONS: These findings are essential for continuous surveillance disease control strategies and monitoring of variants, and thus emphasize on the importance of improving the vaccination program in Iran.

کلیدواژه‌ها [English]

  • Infectious bronchitis
  • phylogenetic
  • Vaccine

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