Effect of Phenylhydrazine-induced hemolytic anemia on morphological changes of renal cortex in adult mice: Protective effect of crocin.

Document Type : Basic Sciences

Authors

1 1Department of Basic Science, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 2Department of Basic Science, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

3 3Razi Vaccine & Serum Research Institute, Agricultural Research, Educationa and Extension Organisation, Karaj, Iran

Abstract

BACKGROUND: Hemolytic anemia and resultant hypoxia can cause a dysfunction in the body functions. Iron delivered from hemolysis of erythrocytes can also stimulate oxidative stress. OBJECTIVES: In this study, we aimed to investigate the protective effect of crocin on renal quality in animals that were exposed against hemolytic anemia induced by phenilhydrazine. METHODS: Forty-nine male and adult mice (20 – 25 g) were grouped within 7 groups. First group was control and treated with normal saline, and test groups were nominated as 2, 3, and 4 and were treated with phenyl hydrazine 2, 4, and 6 mg/100g/48 h (i.p.). Animals in groups 5, 6, and 7 were treated with crocin (200 mg/k/day, i.p.) in addition of 2, 4, and 6 mg/100g/48 h phenyl hydrazine. After 35 days, the blood samples were collected directly from the heart and after centrifugation (5 min and 3000 rpm), the serum samples were subjected to evaluate the Malondialehyde (MAD) level and total antioxidant capacity (TAC), and renal were fixed in 10% formal saline for Paraffin sections. After tissue processing sample staining with H&E method. RESULTS: This study showed that hemolytic anemia effects in proximal tubule of renal cortex, elso showed that the phenylhydrazine administration resulted in a dose-dependent declining of TAC and the crocin administration slightly was able to protect from TAC reduction. Moreover, PHN increased MDA level in serum in a dose-dependent manner, while co-administration of crocin with PHN significantly (P<0.05) reduced the rate of lipid peroxidation.while those groups which were treated with crocin showed a remarkable improvement in testis parameters. CONCLUSIONS: These results indicate a protective effect of Crocin against the anemia-related hypoxia on renal parameters. The protective capacity of Crocin might relate to its known antioxidant power.

Keywords

References

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Journal of Veterinary Research
Volume 73, Issue 1
March 2018
Pages 63-70

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