An Experimental Study of Histopathological Lesions Caused by H9N2 Subtype of Avian Influenza Virus and Ornithobacterium Rhinotracheale Co-Infection

Document Type : Avian (Poultry) Health Management

Authors

1 Meshginshahr Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran

2 Department of Avian Diseases Research and Diagnosis, Razi Vaccine and Serum Research Institute, Karaj, Iran

3 Department of Poultry Diseases, Sciences and Research Branch, Islamic Azad University, Tehran, Iran

4 4Department of Pathology, Razi Vaccine and Serum Research Institute, Karaj, Iran

5 Department of Clinical Sciences, Islamic Azad University, Tabriz Branch, Tabriz, Iran

Abstract

BACKGROUND: Avian influenza virus H9N2 subtype and Ornithobacterium
rhinotracheale (ORT) causes histopathological lesions in birds. Yet, there
is not any study on tissue lesions caused by these pathogens co-infection in
specific pathogen free (SPF) chicks. OBJECTIVES: The aim of this study
was to evaluate the type, severity and extent of histopathological lesions
induced by co-infection of H9N2 subtype avian influenza virus and Ornithobacterium
rhinotracheale in SPF chickens. METHODS: Sixty, one-day-old SPF
chicks were divided randomly into three groups of twenty. At 21 days, the
chicks in the first group were infected with H9N2 subtype AI virus and the
second group was inoculated with H9N2 subtype AI virus and ORT simultaneously.
The third group was inoculated with PBS as control. Then, three birds from each
group were randomly selected and euthanized and autopsied at 2  till 16 days post-inoculation (DPI). The
tissue samples were collected from trachea, lungs, liver, spleen, thymus, kidneys,
cecal tonsil and bursa of fabricius. They were cut into 5 to 6 μm thickness
sections using paraffin embedding method and were stained by Hematoxylin and
Eosin (H&E). RESULTS: The severity of histopathological lesions in
the second group was higher than first group. Tissue changes were not observed
in control group. CONCLUSIONS: The results of this study showed that
infected chickens with H9N2 subtype AI virus and ORT simultaneously cause
exacerbated histopathological lesions compared to H9N2 subtype of AI virus

Keywords

References

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Journal of Veterinary Research
Volume 73, Issue 2
June 2018
Pages 199-207

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