Proteomic Profiling Survey and Identification of Some Immunogenic Proteins From Larvae of Rhipicephalus (Boophilus) annulatus Tick (Mazandaran Strain)

Document Type : Parasitology & Parasitic Diseases

Authors

1 1Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran,Tehran, Iran

2 Rastegar Reference Laboratory, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

3 3Razi Vaccine and Serum Research Institute, Karaj, Iran

Abstract

BACKGROUND: Rhipicephalus (Boophilus) annulatus is an important tick, which can transmit parasites and bacteria to cattle. It can also cause hides damage, growth reduction and sometimes paralysis. So, the tick infestation can be regarded as a major problem in the livestock industry. Different control methods including the chemical ones are used to fight ticks, but because of developing resistance to chemical treatments, researchers try to find some immunogenic proteins for vaccine production. Investigating these tick proteins could be an important step in the identification of biological molecules for the purpose of developing control strategies. OBJECTIVES: The aims of present study were to evaluate and analyze Boophilus annulatus larval extract proteins by 2- dimensional gel electrophoresis patterns (proteomic profiling) and identification of some its immunogenic proteins by two dimensional immunoblotting and  MALDI TOF/TOF mass spectrometry  referred to as Mazandaran strains. METHODS: The steps followed here were: tick preparation and culture, 1-D electrophoresis, and then 2-D electrophoresis, Western blotting and Mass spectrometry and then Mass data were analyzed by Mascot software. RESULTS: Analysis of the produced 2D image identified approximately 80 protein spots with different Molecular weight and PI by Coomassi blue staining. Based on immunogenicity (through Western blotting) and high concentration, 10 protein spots (between 14 and 97 kDa) candidates for MALDI TOF and MALDI TOF - TOF MS.  Among the 10 proteins spots, Vitellogenin, Vitellogenin-2 precursor, tropomyosin, hypotethical protein ISCW001652 were identified proteins with immunogenic properties. Also, quantification analysis showed some proteins had more quantity in soluble larvae protein extract and some such as vitellogenin had some isoforms. CONCLUSIONS: The results of this study could be a preliminary step towards selecting proteins candidated to develop vaccines against ticks.

Keywords

References

Ayuso, R., Reese, G., Leong-Kee, S., Plante, M., Lehrer, S.B. (2002) Molecular basis of arthropod cross-reactivity: IgE-binding cross-reactive epitopes of shrimp, house dust mite and cockroach tropomyosins. Int Arch Allergy Immunol. 129: 38-48.##
Andreotti, R., Pedroso, M.S., Caetano, A.R., Martins, N.F. (2008) Comparison of predicted binders in Rhipicephalus (Boophilus) microplus intestine protein variants Bm86 Campo Grande strain, Bm86 and Bm95. Rev Bras Parasitol Vet. 17(2): 93-98.##
Brossard, M., Wikel, S.K. (2004). Tick immunobiology. Parasitology. 129: 161- 176.##
Brown, S.J., Shapiro, S.Z., Askenase, P.W. (1984) Characterization of tick antigens inducing host immune resistance. J Immunol.132:3319-3325. (PubMed).##
Chen, T.T., Hillen, L. (1983). Expression of the vitellogenin genes in insects. Gamete Research. 7: 179-196.##
Das, S., Banerjee, G., DePonte, K., Marcantonio, N., Kantor, F.S., Fikrig, E. (2001) Salp25D, an Ixodes scapularis antioxidant, is 1 of 14 immunodominant antigens in engorged tick salivary glands. J Infect Dis. 184: 1056-1084. ##
De la Fuente, J., Estrada-Peña A. (2012) Ticks and tick-borne pathogens on the rise. Ticks Tick Borne Dis. 3(3): 115-6.##
Ferreira, C.A., Barbosa, M.C., Silveira, T.C., Valenzuela, J.G., Vaz Ida, S. J., Masuda, A. (2002). cDNA cloning, expression and characterization of a Boophilus microplus paramyosin. Parasitology. 125(3): 265-74.##
Gindin, G., Samish, M., Zangi, G., Mishoutchenko, A., Glazer, L. (2002) The susceptibility of different species and stages of ticks to entomopathogenic fungi. Exp Appl Acarol. 28: 283-288. (PubMed)##
Helfman, D.M., Flynn, P., Khan, P., Saeed, A. (2008) Tropomyosin as a regulator of cancer cell transformation. Adv Exp Med Biol. 644:124-31.##
Hooper, S.L., Thuma, J.B. (2005) Invertebrate muscles: muscle specific genes and proteins. Physiology. 85(3): 1001-60.##
Huntley, J.F., Machell, J., Nisbet, A.J., Vanden, B. A., Chua, K.Y., Cheong, N., Hales, B.J., Thomas, W.R. (2004) Identification of tropomyosin, paramyosin and apolipophorin/ vitellogenin as three major allergens of the sheep scab mite, Psoroptes ovis. Parasite Immunol. 26(8-9): 335-42.##
Jongejan, F., Uilenberg, G. (2004). The global importance of ticks. Parasitol. 129: 3-14.##
Krasky,  A., Rohwer, A., Schroeder,  J., Selzer,  P.M. (2007) A combined bioinformatics and chemoinformatics approach for the development of new antiparasitic drugs. Genomics 89(1): 36-43.##
Lamelli, U.K. (1970) Cleavage of structural proteins during the assembly of head bacteriophage T4.Nature. 227: 680-685.##
Lehrer, S.B., Ayuso, R., Reese, G. (2003) Seafood allergy and allergens: a review. Biotechnol. 5: 339-348.##
Mahadev, K., Raval, G., Bharadwaj, S., Willingham, M.C., Lange, E.M., Vonderhaar, B., Salomon, D., Prasad, G.L. (2002) Suppression of the transformed phenotype of breast cancer by tropomyosin-1. Exp Cell Res. 279(1): 40-51.##
Miller , R., Yeater,  K., Allen,  A., Almazan,  C., Allen, A. , Jory, L., Yeater, K. , Messenger, M. , Ellis, D. (2012) Exploring the use of an anti-tick vaccine as a tool for the integrated eradication of the cattle fever tick, Rhipicephalus (Boophilus) annulatus. Vaccine. 30(38): 5682-5682.##
Mulenga, A., Sugimoto, C., Onuma, M. (2000) Issues in tick vaccine development: identification and characterization of potential candidate vaccine antigens. Microbes Infect. 2: 1351-1361.##
Nabian, S., Taheri, M., Fard, R.M., Aramoon, M. (2013) Identification of tropomyosin and its immunological properties from larvae of cattle tick, boophilus annulatus. Iran J Parasitol. 8(2):242-8.##
Nisbet, A.J., Huntley, J.F., Mackellar, A., Sparks, N., Mc, D.R. (2006) A house dust mite allergen homologue from poultry red mite Dermanyssus gallinae (De Geer). Parasite Immunol. 28: 401-5.##
Nuttall, P.A., Labuda, M. (2004). Tick-host interactions: saliva-activated transmission. Parasitology. 129: 177-189.##
O’Farrell, P.H. (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 250: 4007-21.##
Patrick, C.D. and Hair, J.A. (1975). Laboratory rearing procedures and equipment for multi-host ticks (Acarina: Ixodidae) J Med Entomol. 12: 389-390.##
Seng, P., Drancourt, M., Gouriet, F., La Scola, B., Fournier, P. E., Rolain, J.M., Raoult, D. (2009) Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin Infect Dis. 49(4): 552-3.doi:10.1086/600885. PMID 19583519.##
Tellama, R.L., Kempa, D., Ridinga, G., Briscoea, S., Smithb, D., Sharpb, P., Irvingb, D., Willadsena, P. (2002). Reduced oviposition of Boophilus microplus feeding on sheep vaccinated with vitellin. Vet Parasitol. 103 (1-23): 141-156.##
Untalana, P.M., Guerrero, F.D., Haines, L.R., Pearson, T.W. (2005) Proteome analysis of abundantly expressed proteins from unfed larvae of the cattle tick, Boophilus microplus. Insect Biochem Mol Biol. 35:141-151.##
Valenzuela, J.G. (2002). High-throughput approaches to study salivary proteins and genes from vectors of disease. Insect Biochem Mol Biol. 32: 1199-1209.##
Valenzuela, J.G. (2004) Exploring tick saliva: from biochemistry to “sialomes’ and functional genomics. Parasitology. 129: 83-94. ##
Wang, H., Nuttal, P.A. (1994) Comparison of the proteins in salivary glands, saliva and haemolymph of Ripicephalus appendiculatus female ticks during feeding. Parasitol. 109: 517-523.##
Willadsen, P., Smith, D., Cobon, G., Mckenna, R.V. (1996) Comparative vaccination of cattle against Boophilus micropolus with recombinant antigen Bm86 alone or in combination with recombinant Bm91. Parasite Immunol. 18:241-246.##
Wiladsen, P. (2004) Anti-tick vaccines. J Parasitol. 129: 367-387.##
Xiang, F.Y., Zhang, J.W., Zhou, Y.Z., Li, Z., Gong, H.Y., Zhou, J.L. (2009) Proteomic analysis of proteins in the salivary glands of the fed and unfed female tick Rhipicephalus haemaphysaloides. Agricultural Sciences in China. 8(1): 121-127.##
You, M.J. (2004) Immunization effect of recombinant P27/30 protein expressed in Escherichia coli against the hard tick Haemaphysalis longicornis in rabbits. Korean J Parasitol. 42(4): 195-200.##
Journal of Veterinary Research
Volume 73, Issue 3
September 2018
Pages 265-271

Files

Share

How to cite

Statistics