Endocannabinoid system play a critical role in the regulation of appetite in mammals. In the present study, the effect of avian brain CB1 receptor on food intake of broilers was studied. ACEA, a potent CB1 agonist, and AM281, a potent CB1 antagonist, were injected into the chicken right lateral cerebral ventricle and food intake was measured 15, 30, 60, 120, and 180 minutes post injection. The results indicate that CB1 agonist and antagonist increase and decrease food intake, respectively. Also, pretreatment with CB1 antagonist fully inhibits the CB1-agonist-induced food-intake. The results of the study is consistent with the experiments carried out in mammals.