During sperm storage, Oxidative damage is one of the major causes for the reduction of sperm motility and fertility. This experiment was conducted to compare the effects of different levels of vitamin E and C, as antioxidants on diluted sperm and their protective effects were compared on traits related to the quality of sperm. A two-variable factorial experiment in a completely randomized design(CRD) including seven levels of vitamins C, E and control group along with two levels of storage time was used in this study. The levels of vitamin E, were included E2 (2 mg), E4 (4 mg) and E6 (6 mg) per 10 ml diluted sperm and the levels of vitamin C, were included C0.3(0.3mg),C0.6 (0.6mg)and C0.9(0.9 mg)per 1ml diluted sperm. At initiation of storage there was no significant difference between levels of vitamins E , C and control group (p>0.5). After 72 hours of storage there was a significant difference between percentage of motile spermatozoa in different levels of vitamin E, with other treatments (p<0.05). The viability levels of E4 and E6 were significantly higher (p<0.05) however no significant difference was seen between viability of E2 and E4 (p>0.05). The levels of membrane integrity in E2 and E6 were higher significantly than other groups (p<0.05). According to the results of the present study, supplemented of vitamin E with 6 mg in 10 ml of the diluted sperm based on Tris has the best effect in the quality of sperm of Taleshi ram at 5oC.