THE EFFECT OF DIFFERENT LEVELS OF CADMIUM ON HISTOPATHOLOGICALCHANGES AND THE RATE OF LYMPHOID CELLS APOPTOSIS OF BURSA OF FABRICIUS IN BROLER CHICKENS

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Abstract

Cadmium toxicity can cause kidney failure, liver damage and a weakened immune system in experimental and naturally occurring toxicities. This study was designed to investigate the effects of cadmium (Cd) on the histology and the rate of lymphoid apoptosis in the bursa of fabricius of chicken. One-hundred 20-day-old male Ross broilers were purchased and randomly divided into four groups. The control group (C) received no Cd, whereas groups 1, 2, and 3 had rations administered containing 25, 50 and 100 ppm cadmium as CdCl, respectively. At days 14, 28 and 42, seven chicks from each group were randomly selected and sacrificed. The bursa of Fabricius of each chick was removed, weighed, fixed in 10% buffered formalin and processed for histopathology and assessment of the rate of lymphoid cells apoptosis. The apoptotic cells were demonstrated in paraffin embedded tissue sections using the TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) method. The concentration of Cd in the liver samples was measured by atomic absorption. A reverse correlation between the levels of Cd in the rations and the body weight of the chickens (p<0.01) was found. The concentration of Cd in the liver showed a positive correlation with the levels of Cd in the rations (p

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Journal of Veterinary Research
Volume 66, Issue 3 - Serial Number 3
September 2011
Pages 193-201

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