EFFECTS OF SHORT-TERM STARVATION ON HEMATOLOGICAL PARAMETERS  IN CULTURED JUVENILE BELUGA

Morshedi, Vahid; Ashouri, Ghasem; Khochanian, Parita; Yavari, Vahid; Bahmani, Mahmoud; Pourdehghani, Mohammad; Yazdani, Mohammad Ali; Porali Fashtami, Hamid Reza; Azodi, Maryam

EFFECTS OF SHORT-TERM STARVATION ON HEMATOLOGICAL PARAMETERS IN CULTURED JUVENILE BELUGA

Authors

Abstract

This study examined the effect of different starvation periods on hematological parameters
including hemoglobin(MCV), hematocrit (MCH), red and white blood cells count and corpuscle
indices (MCHC), of cultured juvenile Beluga (Huso huso), with an initial body weight of
45±0.91g. After adaptation to a dry diet for 10 days group s, with 15 fish in each group, were used
for this experiment. Each group was randomly distributed to twelve 500L fiberglass tanks. Each
tank had a flow-through system. The fish were exposed to 4 different feeding regimes; Control
Group (fed four times daily); Group 1 (given 2 days of starvation); Group 2 (given 4 days of
starvation); and , Group 3 (given 8 days of starvation). Analysis was done at the end of each
starvation period by collecting blood from the caudal vein and immediately transporting the
samples to the laboratory. The results of the analysis indicated that the different starvation periods
did not affect the red blood cell count. The hematocrit value in the Control Group was significantly
lower (p<0.05) than the fish group starved for 8 days. MCHC value in the control group was
significantly lower (p<0.05) than the fish group starved for 2 days. The hemoglobin
concentration, MCV and MCH, did not show any significant difference between the treated
groups and the Control Group. Those groups with longer starvation periods showed that the white
blood cell count decreased significantly (p<0.05). It could be concluded that short-term starvation
periods weaken the immunological system of cultured juvenile Beluga.

Keywords