Crimean- Congo hemorrhagic fever (CCHF) is a zoonotic arbovirus disease, which is caused by a virus of Nairovius genus and Bunyaviridae family. The present study was conducted during spring and summer 1384, and blood samples in this study were taken from dairy cow population of Khorasan provinces, including Razavi and Southern Khorasan. All together 248 blood samples belong to four cities of mentioned provinces (Mashad, Torbate heydariyeh, Sarakhs and Birjand) were studied. Prevalence of CCHF virus infection was assessed by enzyme- linked immunosorbent assay (ELISA) for immunoglobulin G (IgG) antibodies to virus. Serologic analysis of serum samples in this study showed that specific IgG against CCHF virus is present in serum samples of 9.6% of all examined samples. Among four sampled cities, anti viral IgG prevalence was highest in Sarakhs (20.7%) and Birjand had the lowest prevalence (3.3%). According to different age groups high prevalence was observed in cows over 5 years old (21.4%) and no specific antibodies found within 2- 3 years old. The presence of serological results for CCHF in dairy cattle from Khorasan province is significant and should be considered in controlling and preventive programs.