Fungal infection of uterine of dairy cattle have not been received much more attention. The aim of this study was: to investigate the uterine mycoflora of dairy cows with reproductive disorders or healthy. Uterine sampling of 112 Holstein dairy cows included 70 cows with reproductive disorders and 42 healthy cows were collected directly. Samples were cultured in sabouraud glucose agar at 28°C for 2 weeks. Data were analyzed using Chi square test. Different fungal agents and yeast were isolated from 22 (31.42%) dairy cows with reproductive disorders, whereas the infection rate was 28.57% in healthy cows. The most common isolated fungi in treatment and control groups were Penicillum (22.72%)and Candida Albicans (41.66), respectively. It is concluded that the uterine of dairy cows with reproductive disorders can be infected by different fungal agents. The rate of fungal contamination and various mycoflora in healthy cows is lower than in dairy cows with reproductive disorders.