In this study a nested-PCR assay was optimized for detection of two BVDV biotype of NADL strain. A part of 5' non-coding region of virus, 249 bp in size, was amplified in RT-PCR. PCR product was cloned in a pTZ57R/T vector and sequencing results confirmed the specificity of the test. Internal primers were designed and a 155 bp DNA fragment was amplified in nested-PCR. The sensitivity of RT-PCR and nested-PCR for detection of virus in cell culture were found to be 104 TCID50 and 102 TCID50, respectively. Seven cell cultures were tested for BVDV contamination using ELISA, RT-PCR and nested-PCR. Results indicate that sensitivity of molecular tests for detection of virus in cell culture samples is higher than ELISA.
Ghorashi, S. A., Daliri Joupari, M., Morshedi, D., Hajian, T. & Lotfi, M. (2008). DETECTION OF PESTIVIRUS CONTAMINATION IN CELL CULTURES BY NESTED-PCR. Journal of Veterinary Research, 63(1), 11–16.
MLA
Ghorashi, S. A., Daliri Joupari, M., Morshedi, D., Hajian, T., & Lotfi, M. "DETECTION OF PESTIVIRUS CONTAMINATION IN CELL CULTURES BY NESTED-PCR", Journal of Veterinary Research, 63, 1, 2008, 11–16.
HARVARD
Ghorashi, S. A., Daliri Joupari, M., Morshedi, D., Hajian, T., & Lotfi, M. (2008). 'DETECTION OF PESTIVIRUS CONTAMINATION IN CELL CULTURES BY NESTED-PCR', Journal of Veterinary Research, 63(1), pp. 11–16.
CHICAGO
Ghorashi, S. A., Daliri Joupari, M., Morshedi, D., Hajian, T. & Lotfi, M., "DETECTION OF PESTIVIRUS CONTAMINATION IN CELL CULTURES BY NESTED-PCR." Journal of Veterinary Research, 63 1 (2008): 11–16,
VANCOUVER
Ghorashi S. A., Daliri Joupari M., Morshedi D., Hajian T., Lotfi M. DETECTION OF PESTIVIRUS CONTAMINATION IN CELL CULTURES BY NESTED-PCR. Journal of Veterinary Research. 2008;63(1):11–16 (In Persian).
