In this study a nested-PCR assay was optimized for detection of two BVDV biotype of NADL strain. A part of 5' non-coding region of virus, 249 bp in size, was amplified in RT-PCR. PCR product was cloned in a pTZ57R/T vector and sequencing results confirmed the specificity of the test. Internal primers were designed and a 155 bp DNA fragment was amplified in nested-PCR. The sensitivity of RT-PCR and nested-PCR for detection of virus in cell culture were found to be 104 TCID50 and 102 TCID50, respectively. Seven cell cultures were tested for BVDV contamination using ELISA, RT-PCR and nested-PCR. Results indicate that sensitivity of molecular tests for detection of virus in cell culture samples is higher than ELISA.
Ghorashi, S. A. , Daliri Joupari, M. , Morshedi, D. , Hajian, T. and Lotfi, M. (2008). DETECTION OF PESTIVIRUS CONTAMINATION IN CELL CULTURES BY NESTED-PCR. Journal of Veterinary Research, 63(1), 11-16.
MLA
Ghorashi, S. A. , , Daliri Joupari, M. , , Morshedi, D. , , Hajian, T. , and Lotfi, M. . "DETECTION OF PESTIVIRUS CONTAMINATION IN CELL CULTURES BY NESTED-PCR", Journal of Veterinary Research, 63, 1, 2008, 11-16.
HARVARD
Ghorashi, S. A., Daliri Joupari, M., Morshedi, D., Hajian, T., Lotfi, M. (2008). 'DETECTION OF PESTIVIRUS CONTAMINATION IN CELL CULTURES BY NESTED-PCR', Journal of Veterinary Research, 63(1), pp. 11-16.
CHICAGO
S. A. Ghorashi , M. Daliri Joupari , D. Morshedi , T. Hajian and M. Lotfi, "DETECTION OF PESTIVIRUS CONTAMINATION IN CELL CULTURES BY NESTED-PCR," Journal of Veterinary Research, 63 1 (2008): 11-16,
VANCOUVER
Ghorashi, S. A., Daliri Joupari, M., Morshedi, D., Hajian, T., Lotfi, M. DETECTION OF PESTIVIRUS CONTAMINATION IN CELL CULTURES BY NESTED-PCR. Journal of Veterinary Research, 2008; 63(1): 11-16.
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