In this study a nested-PCR assay was optimized for detection of two BVDV biotype of NADL strain. A part of 5' non-coding region of virus, 249 bp in size, was amplified in RT-PCR. PCR product was cloned in a pTZ57R/T vector and sequencing results confirmed the specificity of the test. Internal primers were designed and a 155 bp DNA fragment was amplified in nested-PCR. The sensitivity of RT-PCR and nested-PCR for detection of virus in cell culture were found to be 104 TCID50 and 102 TCID50, respectively. Seven cell cultures were tested for BVDV contamination using ELISA, RT-PCR and nested-PCR. Results indicate that sensitivity of molecular tests for detection of virus in cell culture samples is higher than ELISA.
Ghorashi,S A , Daliri Joupari,M , Morshedi,D , Hajian,T and Lotfi,M . (2008). DETECTION OF PESTIVIRUS CONTAMINATION IN CELL CULTURES BY NESTED-PCR. Journal of Veterinary Research, 63(1), 11-16.
MLA
Ghorashi,S A , , Daliri Joupari,M , , Morshedi,D , , Hajian,T , and Lotfi,M . "DETECTION OF PESTIVIRUS CONTAMINATION IN CELL CULTURES BY NESTED-PCR", Journal of Veterinary Research, 63, 1, 2008, 11-16.
HARVARD
Ghorashi S A, Daliri Joupari M, Morshedi D, Hajian T, Lotfi M. (2008). 'DETECTION OF PESTIVIRUS CONTAMINATION IN CELL CULTURES BY NESTED-PCR', Journal of Veterinary Research, 63(1), pp. 11-16.
CHICAGO
S A Ghorashi, M Daliri Joupari, D Morshedi, T Hajian and M Lotfi, "DETECTION OF PESTIVIRUS CONTAMINATION IN CELL CULTURES BY NESTED-PCR," Journal of Veterinary Research, 63 1 (2008): 11-16,
VANCOUVER
Ghorashi S A, Daliri Joupari M, Morshedi D, Hajian T, Lotfi M. DETECTION OF PESTIVIRUS CONTAMINATION IN CELL CULTURES BY NESTED-PCR. Journal of Veterinary Research. 2008;63(1):11-16 (In Persian).
