This study was designed to induce nutritional hypophosphatemia to investigate the possibility of intervascular hemolysis in the control (7 lambs) and test (8 lambs) groups. Phosphorus of the ration for the test and control groups was formulated as 0.063 % and 0.34% of dry matter, respectively. The jugular vein blood samples were weekly taken for inorganic phosphorus, alkaline phosphatase, billirubin, hematocrit and Heinz body examination. The indepentent t-test and repeated measures )analysis of variance) methods applied for the statistical analyses. Significant hypophosphatemia (p<0.01) and increase in the alkaline phosphatase activity (p<0.001) observed in the test lambs. Hienz bodies in the red blood cells and higly significant decrease of the hematocrit (p< 0/0005) appeared in both groups. Although, visible hemoglobinuria was not observed. The results cleares up potential sensitivity of sheep to nutritional hemolysis.