Laparoscopic and open ovariohysterectomy were compared for the following parameters: surgical time, incision length, suture number, saturation of oxyhemoglobin (SpO2), heart rate, complications,CBC findings, Melbourne pain scale (MPS) and gross pathology. MPS pain scores were evaluated using Mann-whitney U Test. Other parameters were evaluated using Studen's t test. There were no significant differences in SPo2, heart rate, surgical complications, MPS pain scores and CBC, where as the blood loss was significantly (P<0.05) lower and the total incision length and suture number was less in laparoscopic group. There were less surgical time and more extensive adhesion formation in the open technique. The laparoscopic ovariohysterectomy is a potentially safe surgical technique in dogs and leads to less adhesion formation that may result in pain of adhesion sites and movment limitation.