Journal of Veterinary Research

Journal of Veterinary Research

A STUDY ON THE MOUTH EPITHELIUM SAMPLES SUBMITTED TO IRAN CENTRAL VETERINARY LABORATORY DURING THE YEAR OF 2000-2001, FOR FMDV INVESTIGATION, USING CFT, ELISA, AND VIRUS ISOLATION AND ITS PERVALENCE IN IRAN

Authors
Masoud Hosseini
Nasrin Mahmoudan
Gholam Ali Kiani
Roghayeh Sedigeh Moghadam
Reza Hassanzadeh
Laleh Moazami
Mohammad Hasan Roostaee
Abstract
The aim pf the present study was to compare three different methods for detection of food and mouth disease virus antigens(A, Asia1, O1)from mouth epithelium samples and also to monitore the distribution of FMDV serotypes 338 cattle and 34 sheep of Iran during 2000-2001. The suspension fluids of processed tongue epithelium samples were clarified by centrifugation and tested for presence of FMDV antigens. using Complement Fixation Test(CFT), Enzyme-linked Immuno-sorbant assay (ELISA) and inoculating onto BHK-21 cell culture. 151 out of 372 (40%) epithelium samples were positive to one of the FMD viruses, using CFT. These samples were collected from most provinces of Iran during one year (200-2001). The virus was isolated from 78 out of 100 mouth epithelium samples in BHK-21 cell culture. The CF and ELISA tests were positive in 25 (30%) and 50(64%), respectively. ELISA was at least two times more sensitive than the CFT for detection of FMDV in epithelial samples. The Northwest region showed the highest rate of infection in the country meanwhile, Tehran province had the highest rate of infection among provinces.
Keywords
CFT
ELISA
foot-and-mouth disease virus
Virus isolation
Journal of Veterinary Research
Volume 62, Issue 3
Autumn 2007
Pages 251-255