Objective: Isolation of some bacteria species capable of degrade wheat straw lignin and polysaccharides.
Design: Completely rondomizied design with factorial experiment. Animals: The collected Termites including bacteria from their guts. Procedure: Presenting reports related to distribution pattern and species of Termites in Markazy province (Saveh area) in Iran. The collected samples(Termites) were transported to the diagnostic laboratory for primary identification. Then, samples were sent to the reference laboratory in Ontario, Canada for further identification approval. From cultured Termites in laboratory bacteria were isolated by selection method. Kinds of lignin was extracted from wheat straw were used as specific media in isolation of bacteria. After isolation of bacteria, they identified by our and then by reference laboratory. Determination of growth curve and optimum temperature and pH for isolated bacteria performed by present methods. Stastical analysis: Growth curve, ANaYA, optimum pH and temperature by CRD design and factorial experiment were analysied. Results: The collected Termites were identified as Anacanthotermes vagans. Three of the isolates, tentatively identified as Bacillus sp., Enterobacter sp., and Ocrobacterium sp., were capable of utilizing all three lignin preparations as well as extracted wheat strawas a sole source of carbon. Between the selected bacteria, Enterobacter had more and faster growth rate than the other two species. The results showed that the isolated bacteria prefer 40°c and nuetral pH for their growth.
Conclusion: The isolated bacteria makes the biodegradation of wheat straw and other similar lignin containing biological waste products commercially feasible. J. Fae. Vet. Met!. Univ. Tehran. 58, 3: 249-256, 2003.