Objective: To determine of a proper time to de-epithelialization of ileal segment by using collagenase and trypsin cocktail for ileocystoplasty.
Design: Descriptive experimental study. Animals: Fifteen adult female mixed Persian dogs.
Procedure: Ileocystoplasty was performed on 15 female Persian mixed breed dogs ranging from 2 to 3 years old and 15 to 24 kg body weight. Twenty centimeters of ileal segment with its adequate mesentery was selected. Five millimeters of ileal segment was taken for normal histological study of the intestine. Enzymatic
treatment of ileal segment using 0.125% collagenase and trypsin cocktail in 5, 10, 15, 20 and 25 minutes was performed on dogs. After each enzymatic exposure, tissue samples (5 mm) were taken for histopathologic evaluation.
Results: Microscopical study of 180 histologic sections showed
that 5 minutes treatment (MT), focally denuded 20% of villi epithelium; while 10 MT has caused 33% of villi epithelium focally. In 15 MT approximately 60% of villi were de-epithelialized while 20 MT caused 75% villi de-nudation with more cryptlysis. Complete de-nudatson of villi epithelium occurred with 25 MT.
Clinical implications: The results of this preliminary study state
reveal that approximately 25 minutes enzymatic treatment of ileal segment can completely de-epithelialize villi, and is recommended for ileocystoplasty. J. Fac. Vet. Med. Univ. Tehran. 59, 3: 207-
214, 2004.