Objective: To study the presence of spy genes among different
Salmonella serovars that isolated m veterinaiy microbiology departement
Design: Observation study.
Samples : A total of 138 Salmonella strains belonged to 9 different serovars were studied.
Procedure: In this study we applied PCR method using PG44 and
PG48 primers to amplify spvR gene in different serotypes. Results: In PCR amplification, serotyps S.abortusovis, S.dublin, S.typhimurium and S.brandburg developed the 890 bp amplicons.
S.typhi, S.senftenberg and S.bovismorb,ficans have yielded
nonspecific bands of different sizes. S.newport revealed no band
Conclusion: Salmonella serotypes such as typhi, senftenberg and bovismorb/lcans with nonspecific bands in PCR amplification does not share virulence plasmids. Furthermore, spvR loci could
be considered as a good marker for presence or absence of virulence
plasmids in different Salmonella serotypes.