Objective: The purpose of this study was to designing a simple, sensetive and cheep method based on dot ELISA system for detecting of seroconversion to infectious bovine rhinotracheitis virus (IBRV). Procedure: In the first step rabbit antibovine immunoglobins was conjugated with horseradish peroxidas and purifide IBR virus was used as coated antigen on nitrocellulose membrane. In order to standardization of the kit, 50 positive and 50 negative sera that tested by comercial ELISA kit (Svanova) and evaluated the best dilution of the materials that used in the test.
Results: The optimum dilution of different compound of the dot ELISA kit were: 1 :200 for HRPO conjugated antiglobulin, 1:8 for serum and 1 for tetramethylbenzidin as substrate.
In order to evaluation of correlation of dot ELISA method with comercial ELISA kits, 488 serum samples were tested by two mentioned testes.
Statistical analysis: Chi squire test.
Conclusion: Dot ELISA have revealed 88/9% corrlation with plate ELISA and no significant difference between results of this two tests. J. Fac. Vet. Med. Univ. Tehran. 58, 4: 383-387, 2003