Sixteen seronegative dogs were randomly divided into two groups. All of the 8 dogs of group I recived an intraperitoneal challenge of 2.5 106 infective promastigotes of L. infantum (MCAN/1R194/Moheb 1). All dogs of Group I and group 2 were tested from 1 to 5 month after challenge by ELISA and IFA techniques for detecting anti-leishmania antibodies. Necropsy was performed on all dogs to investigate for parasites. A complete correlation was observed between ELISA and parasitological procedures.