The macroscopic studies showed that buffalo small intestine contain two types of peyer’s patches ie. discrete peyer’s patches in the total lenght of Jejunum - ileam (JPP) and a single continuous ileal peyer’s patche in the terminal part of ileum (IPP). The JPP have elliptical shapes and were scattered on mucosal surface of jejunum - ileum. The number of JPP in the cranial part were more than termiani part (P <0.05). The area of JPP in the terminal part of jejunum- ileum in 3-4 years old were more than 6-24 months old (P <0.05). The ileal peyer’s patche was well developed in 6 - 18 months old and after this age, the regression was started, as in macroscopic observations only a few folds were seen in 24 months old and there was no evidence of ileal peyer’s patche in 2.5 years old. The microscopic studies showed that the JPP consisted of pear shape follicles which mainly had placed in submucosa and some of them
had extended to the lamina propria. The interfollicular space were occupy by diffuse lymphoid tissue, There were not seen any regression in the follicles of the JPP in different ages. The IPP consisted of long - sac shape follicles which mainly had placed in submucosa and they rarely penetrated to the lamina propria. The more intersting finding was the regression of IPP, as the number and size of follicles were decreased by age. This regression was more significant in 3- 4 years old buffalo as only a few dispersed follicles were seen in this age.
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