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Abstract

The effects of pH (5 to 7.4) , sodium chloride (NaCl, 0.5 to 3%), potassium sorbate (KS, 0.0 to 3%) and temperature (T, 15 to 35°C) on Lag time (Lag) of growth of Salmonella typhimurium in a model broth (Brain Heart Infusion, BHI) were evaluated in a factorial
design study. The lag time of growth of S. typhimurium was affected
significantly (P < 0.002) by the values of KS, pH , T but not by NaC1,
concentration used in this study (p = 0.5). Regression equation was
derived relating Lag to KS , NaC1, T and pH. From this model the
‘alues of predicted Lag time of S. typhimurium can be calculated
from any combination of KS, NaC1, pH and T within the limits
studied (R2 = 0.82).

Keywords